Molecular characterization of the most common dsRNA element from Trichoderma atroviride indicated that it comprised 8,566 bp and encoded two large open reading frames (ORF1 and 2). The two ORFs were found to overlap by 46 bp with a typical (−1) slippery heptanucelotide sequence. The deduced protein sequences of ORF1 and ORF2 showed significant similarities to those of known mycoviral structural proteins and RNA-dependent RNA polymerases, respectively. Phylogenetic analysis indicated that this dsRNA is a member of a distinct species related to a group of unclassified mycoviruses; therefore, it was named Trichoderma atroviride mycovirus 1 (TaMV1).
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This work was supported by the Bio-industry Technology Development Program, Ministry for Food, Agriculture, Forestry and Fisheries, Republic of Korea and in part by the NRF grant by MSIP (2015R1A2A1A10055684). We thank the Institute of Molecular Biology and Genetics at Chonbuk National University for kindly providing the facilities for this research. D-H Kim was supported by “Research Base Construction Fund Support Program” funded by Chonbuk National University in 2016.
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This article does not contain any experiments involving human participants or animals.
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Lee, S.H., Yun, SH., Chun, J. et al. Characterization of a novel dsRNA mycovirus of Trichoderma atroviride NFCF028. Arch Virol 162, 1073–1077 (2017). https://doi.org/10.1007/s00705-016-3214-z
- Trichoderma atroviride
- Lentinula edodes