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Characterization of a novel dsRNA mycovirus of Trichoderma atroviride NFCF028

Abstract

Molecular characterization of the most common dsRNA element from Trichoderma atroviride indicated that it comprised 8,566 bp and encoded two large open reading frames (ORF1 and 2). The two ORFs were found to overlap by 46 bp with a typical (−1) slippery heptanucelotide sequence. The deduced protein sequences of ORF1 and ORF2 showed significant similarities to those of known mycoviral structural proteins and RNA-dependent RNA polymerases, respectively. Phylogenetic analysis indicated that this dsRNA is a member of a distinct species related to a group of unclassified mycoviruses; therefore, it was named Trichoderma atroviride mycovirus 1 (TaMV1).

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References

  1. 1.

    Tokimoto K (1985) Physiological studies on antagonism between Lentinula edodes and Trichoderma spp. in bedlogs of the former (in Japanese). Rep Tottori Mycol Inst 23:1–54

    Google Scholar 

  2. 2.

    Ulhoa CJ, Peberdy JF (1992) Purification and some properties of the extracellular chitinase produced by Trichoderma harzianum. Enzyme Microb Technol 14:236–240

    CAS  Article  Google Scholar 

  3. 3.

    Seaby D (1998) Trichoderma as a weed mould or pathogen in mushroom cultivation. In: Harmann GE, Kubicek CP (eds) Trichoderma and gliocladium, vol 2. Taylor and Francis Ltd, London, pp 267–288

    Google Scholar 

  4. 4.

    Kredics L, Garcia Jimenez L, Naeimi S, Czifra D, Urban P, Manczinger L, Vagvolgyi C, Hatvani L (2010) A challenge to mushroom growers: the green mould disease of cultivated champignons. In: Mendez-Vilas A (ed) Current research, technology and education topics in applied microbiology and microbial biotechnology, vol 1. Formatex Research Center, Badajoz, pp 295–305

    Google Scholar 

  5. 5.

    Van Alfen NK (1986) Hypovirulence of Endothia (Cryphonectria) parasitica and Rhizoctonia solani. In: Buck KW (ed) Fungal virology. CRC Press, Boca Raton, pp 143–162

    Google Scholar 

  6. 6.

    Nuss DL, Koltin Y (1990) Significance of dsRNA genetic elements in plant pathogenic fungi. Annu Rev Phytopathol 28:37–58

    CAS  Article  PubMed  Google Scholar 

  7. 7.

    Wickner RB (1992) Double-stranded and single-stranded RNA viruses of Saccharomyces cerevisiae. Annu Rev Microbiol 46:347–375

    CAS  Article  PubMed  Google Scholar 

  8. 8.

    Kim DH, Yun SH, Lee SH, So KK, Kim JM (2016) Incidence of diverse dsRNA mycoviruses in Trichoderma spp. causing green mold disease of shiitake Lentinula edodes. FEMS Microbiol Lett. doi:10.1093/femsle/fnw220

    Google Scholar 

  9. 9.

    Xie J, Xiao X, Fu Y, Liu H, Cheng J, Ghabrial SA, Li G, Jiang D (2011) A novel mycovirus closely related to hypoviruses that infects the plant pathogenic fungus Sclerotinia sclerotiorum. Virology 418:49–59

    CAS  Article  PubMed  Google Scholar 

  10. 10.

    Wang M, Wang Y, Sun X, Cheng J, Fu Y, Liu H, Jiang D, Ghabrial SA, Xie J (2015) Characterization of a novel megabirnavirus from Sclerotinia sclerotiorum reveals horizontal gene transfer from single-stranded RNA virus to double-stranded RNA virus. J Virol 89:8567–8579

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  11. 11.

    Marvelli RA, Hobbs HA, Li S, McCoppin NK, Domier LL, Hartman GL, Eastburn DM (2014) Identification of novel double-stranded RNA mycoviruses of Fusarium virguliforme and evidence of their effects on virulence. Arch Virol 159:349–352

    CAS  Article  PubMed  Google Scholar 

  12. 12.

    Brierley I, Boursnell ME, Binns MM, Bilimoria B, Blok VC, Brown TD, Inglis SC (1987) An efficient ribosomal frame-shifting signal in the polymerase-encoding region of the coronavirus IBV. EMBO J 6:3779–3785

    CAS  PubMed  PubMed Central  Google Scholar 

  13. 13.

    Brierley I, Pennell S, Gilbert RJ (2007) Viral RNA pseudoknots: versatile motifs in gene expression and replication. Nat Rev Microbiol 5:598–610

    CAS  Article  PubMed  Google Scholar 

  14. 14.

    Cai G, Krychiw JF, Myers K, Fry WE, Hillman BI (2013) A new virus from the plant pathogenic oomycete Phytophthora infestans with an 8 kb dsRNA genome: the sixth member of a proposed new virus genus. Virology 435:341–349

    CAS  Article  PubMed  Google Scholar 

  15. 15.

    Wang L, Zhang J, Zhang H, Qui D, Guo L (2016) Two novel relative double-stranded RNA mycoviruses infecting Fusarium poae strain SX63. Int J Mol Sci 17:641

    Article  PubMed Central  Google Scholar 

  16. 16.

    Salaipeth L, Chiba S, Eusebio-Cope A, Kanematsu S, Suzuki N (2014) Biological properties and expression strategy of Rosellinia necatrix megabirnavirus 1 analyzed in an experimental host, Cryphonectria parasitica. J Gen Virol 95:740–750

    CAS  Article  PubMed  Google Scholar 

  17. 17.

    Kozlakidis Z, Hacker CV, Bradley D, Jamal A, Phoon X, Webber J, Brasier CM, Buck KW, Coutts RHA (2009) Molecular characterization of two novel double-stranded RNA elements from Phlebiopsis gigantean. Virus Genes 39:132–136

    CAS  Article  PubMed  Google Scholar 

  18. 18.

    Prospero S, Conedera M, Heiniger U, Rigling D (2006) Saprophytic activity and sporulation of Cryphonectria parasitica on dead chestnut wood in forests with naturally established hypovirulence. Phytopathology 96:1337–1344

    CAS  Article  PubMed  Google Scholar 

  19. 19.

    Suzuki N, Maruyama K, Moriyama M, Nuss DL (2003) Hypovirus papain-like protease p29 functions in trans to enhance viral double-stranded RNA accumulation and vertical transmission. J Virol 77:11697–11707

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  20. 20.

    Sun L, Nuss DL, Suzuki N (2006) Synergism between a mycoreovirus and a hypovirus mediated by the papain-like protease p29 of the prototypic hypovirus CHV1-EP713. J Gen Virol 87:3704–3714

    Article  Google Scholar 

  21. 21.

    Nei M, Kumar S (2000) Molecular evolution and phylogenetics. Oxford University Press, New York

    Google Scholar 

  22. 22.

    Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425

    CAS  PubMed  Google Scholar 

  23. 23.

    Zuckerkandl E, Pauling L (1965) Evolutionary divergence and convergence in proteins. In: Bryson V, Vogel HJ (eds) Evolving genes and proteins. Academic Press, New York, pp 97–166

    Chapter  Google Scholar 

  24. 24.

    Sneath PHA, Sokal RR (1973) Numerical taxonomy. Freeman, San Francisco

    Google Scholar 

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Acknowledgements

This work was supported by the Bio-industry Technology Development Program, Ministry for Food, Agriculture, Forestry and Fisheries, Republic of Korea and in part by the NRF grant by MSIP (2015R1A2A1A10055684). We thank the Institute of Molecular Biology and Genetics at Chonbuk National University for kindly providing the facilities for this research. D-H Kim was supported by “Research Base Construction Fund Support Program” funded by Chonbuk National University in 2016.

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Correspondence to Dae-Hyuk Kim.

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Lee, S.H., Yun, SH., Chun, J. et al. Characterization of a novel dsRNA mycovirus of Trichoderma atroviride NFCF028. Arch Virol 162, 1073–1077 (2017). https://doi.org/10.1007/s00705-016-3214-z

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Keywords

  • Trichoderma atroviride
  • Mycovirus
  • Lentinula edodes
  • Shiitake