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Characterization of apple stem grooving virus and apple chlorotic leaf spot virus identified in a crab apple tree

Abstract

Apple stem grooving virus (ASGV), apple chlorotic leaf spot virus (ACLSV), and prunus necrotic ringspot virus (PNRSV) were identified in a crab apple tree by small RNA deep sequencing. The complete genome sequence of ACLSV isolate BJ (ACLSV-BJ) was 7554 nucleotides and shared 67.0%-83.0% nucleotide sequence identity with other ACLSV isolates. A phylogenetic tree based on the complete genome sequence of all available ACLSV isolates showed that ACLSV-BJ clustered with the isolates SY01 from hawthorn, MO5 from apple, and JB, KMS and YH from pear. The complete nucleotide sequence of ASGV-BJ was 6509 nucleotides (nt) long and shared 78.2%-80.7% nucleotide sequence identity with other isolates. ASGV-BJ and the isolate ASGV_kfp clustered together in the phylogenetic tree as an independent clade. Recombination analysis showed that isolate ASGV-BJ was a naturally occurring recombinant.

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Correspondence to Yongqiang Li or Qi Zhou.

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This research was financially supported by the Beijing Municipal Commission of Education (KM201610020008) and the support program of General Administration of Quality Supervision, Inspection and Quarantine of the People’s Republic of China (201310068, 2015IK022).

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All the authors declare that we have no conflict of interest.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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Li, Y., Deng, C., Bian, Y. et al. Characterization of apple stem grooving virus and apple chlorotic leaf spot virus identified in a crab apple tree. Arch Virol 162, 1093–1097 (2017). https://doi.org/10.1007/s00705-016-3183-2

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  • DOI: https://doi.org/10.1007/s00705-016-3183-2

Keywords

  • Complete Genome Sequence
  • Movement Protein
  • Nucleotide Sequence Identity
  • Apple Stem Groove Virus
  • Apple Chlorotic Leaf Spot Virus