Archives of Virology

, Volume 162, Issue 3, pp 669–675 | Cite as

Influenza virus infection induces translocation of apoptosis-inducing factor (AIF) in A549 cells: role of AIF in apoptosis and viral propagation

  • Xinyan Qu
  • Xiaoran Ding
  • Ming Duan
  • Jing Yang
  • Ruxian Lin
  • Zhe ZhouEmail author
  • Shengqi WangEmail author
Original Article


It is recognized that influenza virus induces caspase-dependent apoptosis by activating caspase-3. Apoptosis-inducing factor (AIF) is a caspase-independent cell death effector, and its mitochondrial-nuclear translocation plays an important role in apoptosis. It is demonstrated in this study how influenza virus infection can induce caspase-independent apoptosis in the human alveolar epithelial cell line A549. AIF is translocated from the mitochondria to the nucleus in a caspase-independent manner in response to infection with influenza virus. Knockdown of AIF expression by small interfering RNA (siRNA) led to a reduction in virus-infection-induced apoptosis and virus yield. These results indicate that AIF translocation has a role in influenza-virus-induced apoptosis.


A549 Cell Influenza Virus Mitochondrial Membrane Potential Viral Titer Influenza Virus Infection 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



This work was supported by a grant from the National Nature Science Foundation of China (No. 81230089).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.


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Copyright information

© Springer-Verlag Wien 2016

Authors and Affiliations

  1. 1.Beijing Institute of Radiation MedicineBeijingChina
  2. 2.Shandong Analysis and Test CenterShandong Academy of SciencesJinanChina
  3. 3.Institute of ZoonosesJilin UniversityChangchunChina

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