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Development of a fluorescence resonance energy transfer–based intracellular assay to identify novel enterovirus 71 antivirals

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Abstract

Enterovirus 71 (EV71) is considered one of the most virulent pathogens in the family Picornaviridae. However, there have been no effective treatments for the severe complications caused by EV71. Development of new drugs against targets that are essential for viral replication often requires screening large collections of compounds, for which a high-throughput screening platform is needed. In this study, a drug-screening platform was developed based on a genetically engineered cell line that displays fluorescence resonance energy transfer (FRET) and shows a real-time and quantifiable impairment of FRET upon EV71 infection. A library of small molecules consisting of 1280 compounds with defined bioactivities was used for screening drugs with anti-EV71 activity; accurate, rapid, and robust results were obtained from this screening procedure. Ten drugs were identified in the primary screening, and their antiviral activities were indicated by dose-dependent elevation of FRET. Among these, AC-93253, mitoxantrone and N-bromoacetamide had not been reported as enterovirus inhibitors, and it was confirmed that they were able to suppress viral yields in a dose-dependent manner. Taken together, these studies demonstrate the feasibility of this FRET-based platform for efficient screening and identification of novel compounds with activity against EV71 infection.

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Acknowledgements

This study was funded by the Ministry of Education of Taiwan (a grant from Ministry of Education, Aim for the Top University Plan [Grant 104AC-D104]) and the Cheng Hsin General Hospital Foundation (Grant 102-61).

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Authors and Affiliations

  1. Department of Clinical Pathology, Cheng Hsin General Hospital, Taipei, Taiwan, R.O.C.

    Wen-Wen Lu

  2. Department of Biotechnology and Laboratory Science in Medicine, National Yang-Ming University, Taipei, Taiwan, R.O.C.

    Fang-Yen Kung, Po-An Deng, Yu-Chu Lin & Szu-Hao Kung

  3. Department of Medical Laboratory Science and Biotechnology, China Medical University, Taichung, Taiwan, R.O.C.

    Cheng-Wen Lin

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  1. Wen-Wen Lu
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  2. Fang-Yen Kung
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  6. Szu-Hao Kung
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Correspondence to Szu-Hao Kung.

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Lu, WW., Kung, FY., Deng, PA. et al. Development of a fluorescence resonance energy transfer–based intracellular assay to identify novel enterovirus 71 antivirals. Arch Virol 162, 713–720 (2017). https://doi.org/10.1007/s00705-016-3143-x

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  • DOI: https://doi.org/10.1007/s00705-016-3143-x

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