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Molecular characterization of a novel endornavirus from the phytopathogenic fungus Botrytis cinerea

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Abstract

The complete sequence of a novel endornavirus (Botrytis cinerea endornavirus 1, BcEV1) from the phytopathogenic fungus Botrytis cinerea strain HBtom-372 was determined. The BcEV1 coding strand is 11,557 nucleotides long, possessing an open reading frame (ORF) that codes for a polyprotein of 3,787 amino acid residues and lacks a site-specific nick. The polyprotein contains viral methyltransferase (MTR) domain, a cysteine-rich region (CRR), two putative viral helicase (DEXDc-like and Hel-1) domains, and an RNA-dependent RNA polymerase_2 (RdRp_2) domain. In phylogenetic analysis, BcEV1 clustered with several fungal endornaviruses, forming an independent clade, and it was detected in 4.2 % of B. cinerea strains collected from central China.

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Acknowledgments

This research was supported by the fundamental research funds for the central universities (Grant no. 0900206185) and the R & D Special Fund for Public Welfare Industry (Agriculture) of China (Grant number 201303025). We greatly appreciate the assistance of Dr. Weidong Chen of Washington State University for proofreading of the manuscript. We also wish to thank Dr. Sead Sabanadzovic and two reviewers for their valuable comments.

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Correspondence to Mingde Wu.

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Hao, F., Zhou, Z., Wu, M. et al. Molecular characterization of a novel endornavirus from the phytopathogenic fungus Botrytis cinerea . Arch Virol 162, 313–316 (2017). https://doi.org/10.1007/s00705-016-3106-2

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