Complete nucleotide sequence of a new filamentous phage, Xf109, which integrates its genome into the chromosomal DNA of Xanthomonas oryzae
- 320 Downloads
Unlike Ff-like coliphages, certain filamentous Inoviridae phages integrate their genomes into the host chromosome and enter a prophage state in their infectious cycle. This lysogenic life cycle was first reported for Xanthomonas citri Cf phage. However, except for the X. citri phages Cf and XacF1, complete genome sequence information about lysogenic Xanthomonas phages is not available to date. A proviral sequence of Xf109 phage was identified in the genome of Xanthomonas oryzae, the rice bacterial blight pathogen, and revived as infectious virions to lysogenize its host de novo. The genome of Xf109 phage is 7190 nucleotides in size and contains 12 predicted open reading frames in an organization similar to that of the Cf phage genome. Seven of the Xf109 proteins show significant sequence similarity to Cf and XacF1 phage proteins, while its ORF4 shares 92 % identity with the major coat protein of X. phage oryzae Xf. Integration of Xf109 phage DNA into the host genome is site-specific, and the attP/attB sequence contains the dif core sequence 5’-TATACATTATGCGAA-3’, which is identical to that of Cf, XacF1, and Xanthomonas campestris phage ϕLf. To my knowledge, this is the first complete genome sequence of a filamentous bacteriophage that infects X. oryzae.
KeywordsComplete Genome Sequence Yersinia Pestis Filamentous Phage attP Site Replication Initiation Protein
I thank Mr. Gregory Contreras for his help with data analysis and English editing.
Compliance with ethical standards
This study was supported by Career Development and Bridge Research Fund from Auxergen.
Conflict of interest
The author declares no conflict of interest.
This article does not contain any study with human participants performed by the author.
- 17.Kuo TT, Huang TC, Wu RY, Yang CM (1967) Characterization of three bacteriophages of Xanthomonas oryzae (Uyeda Et Ishiyama) Dowson. Bot Bull Acad Sin 8:246–254Google Scholar
- 22.Lee SJ (1991) Sequences that mediate the integration of ϕLf DNA into the chromosome of Xanthomonas campestris pv. campestris. National Chung Hsing University, National Chung Hsing UniversityGoogle Scholar
- 35.Yang MK, Su WC, Kuo TT (1991) Highly efficient transfection of Xanthomonas campestris by electroporation. Bot Bull Acad Sin 32:197–203Google Scholar