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Characterization of a new begomovirus and betasatellite associated with chilli leaf curl disease in India

Archives of Virology volume 162, pages 561–565 (2017)Cite this article

Abstract

Chilli leaf curl disease (ChiLCD) is a serious problem and a major limitation to chilli (Capsicum spp.) cultivation in India. Leaves of a chilli plant showing leaf curl symptoms were collected from the Gonda district in Uttar Pradesh, India, in April, 2013. Full-length genomes of a begomovirus and an associated betasatellite were amplified, cloned and sequenced. The size of the begomovirus genome and the betasatellite were 2760 bp and 1374 bp, respectively. The nucleotide sequence of the begomovirus genome shared maximum identity (89 %) with pepper leaf curl Bangladesh virus-India isolate Chhapra (PepLCBV, JN663853), below the threshold for species demarcation. Sequence analysis showed that the begomovirus is a potential recombinant between viruses related to PepLCBV and chilli leaf curl virus (ChiLCV). The name chilli leaf curl Gonda virus (ChiLCGV) is being proposed. The betasatellite associated with ChiLCGV was identified as tomato leaf curl Bangladesh betasatellite (ToLCBDB). Agroinoculation of the viral genome along with betasatellite induced severe leaf curl symptoms in Nicotiana benthamiana. ChiLCGV and ToLCBDB characterized in this study represent a new begomovirus-betasatellite complex infecting Capsicum.

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Acknowledgments

The authors acknowledge the support of Department of Science and Technology (Govt. of India) under DST-FIST programme to Department of Biosciences, JMI. ZAK is thankful to University Grant Commission (Govt. of India) and Ministry of Minority Affairs (Govt. of India) for Maulana Azad National Fellowship for pursuing his Ph.D.

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  1. Plant Virus Laboratory, Department of Biosciences, Jamia Millia Islamia (Central University), New Delhi, 110025, India

    Zainul A. Khan & Jawaid A. Khan

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  1. Zainul A. Khan
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  2. Jawaid A. Khan
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Correspondence to Zainul A. Khan.

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Khan, Z.A., Khan, J.A. Characterization of a new begomovirus and betasatellite associated with chilli leaf curl disease in India. Arch Virol 162, 561–565 (2017). https://doi.org/10.1007/s00705-016-3096-0

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  • DOI: https://doi.org/10.1007/s00705-016-3096-0

Keywords

  • Infectious Clone
  • Begomovirus Species
  • Monopartite Begomoviruses
  • Recombination Detection Program
  • Begomovirus Genome