We isolated two distinct viruses from mosquitoes collected in Bustos, Bulacan province, Philippines, in 2009. These viruses show rapid replication and strong cytopathic effects in mosquito C6/36 cells. Whole-genome analysis of these viruses demonstrated that both viruses belong to the negevirus group. One of the viruses, from Culex vishunui mosquitoes, is a new strain of Negev virus. The other virus, from a Mansonia sp. mosquito, is a new negevirus designated Bustos virus. Gene expression analysis of the Bustos virus revealed that infected cells contain viral subgenomic RNAs that probably include open reading frame (ORF) 2 or ORF3. Purified Bustos virus particles contained at least three proteins, and the major component (a probable major capsid protein) is encoded by ORF3. Bustos virus did not show infectivity in mammalian BHK-21 cells, suggesting that it is an insect-specific virus, like other known negeviruses.
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This research was supported by JSPS KAKENHI Grant Number JP25305010 and the Research Program on Emerging and Re-emerging Infectious Diseases from Japan Agency for Medical Research and Development, AMED.
All authors declare that they have no conflict of interest. All of the animal procedures were conducted in accordance with the guidelines of the National Institute of Infectious Diseases in Japan. This article does not contain any studies with human participants performed by any of the authors.
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Fujita, R., Kuwata, R., Kobayashi, D. et al. Bustos virus, a new member of the negevirus group isolated from a Mansonia mosquito in the Philippines. Arch Virol 162, 79–88 (2017). https://doi.org/10.1007/s00705-016-3068-4
- Nucleotide Position
- Amino Acid Position
- Subgenomic RNAs
- Virus ORF1
- Mosquito Pool