Archives of Virology

, Volume 161, Issue 12, pp 3549–3553 | Cite as

Detection of koala retrovirus subgroup B (KoRV-B) in animals housed at European zoos

  • Uwe Fiebig
  • Martina Keller
  • Joachim DennerEmail author
Brief Report


Many koalas carry an endogenous retrovirus, KoRV-A, in their genome. Recently, a second retrovirus, KoRV-B, was detected in koalas in Japanese and U.S. zoos. However, this virus is not endogenous, differs in the receptor binding site of the surface envelope protein, and uses a receptor different from that of KoRV-A. We describe here a KoRV-B found in koalas at zoos in Germany and Belgium that differs slightly from that found in the Los Angeles zoo.


Retroviruses Endogenous retroviruses Koala PCR 



We thank Francis Vercammen, Royal Zoological Society of Antwerp, and Manuel Garcia Hartmann, Zoo Duisburg, for material from koalas. We thank Dr. S. Norley, Robert Koch Institute, for critical reading of the manuscript.

Compliance with ethical standards

Conflict of interest

All authors declare that they have no conflict of interest.

Ethical approval

This study does not involve animal experiments performed by the authors: only blood and tissue samples from animals housed at the zoos in Antwerp and Duisburg were used, for which all applicable international, national, and/or institutional guidelines for the care and use of animals were followed. No studies with human participants were performed.


  1. 1.
    Anai Y, Ochi H, Watanabe S, Nakagawa S, Kawamura M, Gojobori T, Nishigaki K (2012) Infectious endogenous retroviruses in cats and emergence of recombinant viruses. J Virol 86:8634–8644PubMedPubMedCentralGoogle Scholar
  2. 2.
    Athas GB, Choi Prabhu S, Lobelle-Rich P, Levy L (1995) Genetic determinants of feline leukemia virus-induced multicentric lymphomas. Virology 214:431–438PubMedGoogle Scholar
  3. 3.
    Athas GB, Lobelle-Rich P, Levy LS (1995) Function of a unique sequence motif in the long terminal repeat of feline leukemia virus isolated from an unusual set of naturally occurring tumors. J Virol 69:3324–3332PubMedPubMedCentralGoogle Scholar
  4. 4.
    Bechtel MK, Hayes KA, Mathes LE, Pandey R, Stromberg PC, Roy-Burman P (1999) Recombinant feline leukemia virus (FeLV) variants establish a limited infection with altered cell tropism in specific-pathogen-free cats in the absence of FeLV subgroup A helper virus. Vet Pathol 36:91–99PubMedGoogle Scholar
  5. 5.
    Brown AS, Girjes AA, Lavin MF, Timms P, Woolcock JB (1987) Chlamydial disease in koalas. Aust Vet J 64:346–350PubMedGoogle Scholar
  6. 6.
    Contini C, Fainardi E, Seraceni S, Granieri E, Castellazzi M, Cultrera R (2003) Molecular identification and antibody testing of Chlamydophila pneumoniae in a subgroup of patients with HIV-associated dementia complex. Preliminary results. J Neuroimmunol 136:172–177PubMedGoogle Scholar
  7. 7.
    Cui J, Tachedjian G, Tachedjian M, Holmes EC, Zhang S, Wang LF (2012) Identification of diverse groups of endogenous gammaretroviruses in mega- and microbats. J Gen Virol 93:2037–2045PubMedGoogle Scholar
  8. 8.
    Cui J, Tachedjian M, Wang L, Tachedjian G, Wang LF, Zhang S (2012) Discovery of retroviral homologs in bats: implications for the origin of mammalian gammaretroviruses. J Virol 86:4288–4293PubMedPubMedCentralGoogle Scholar
  9. 9.
    Denner J, Specke V, Thiesen U, Karlas A, Kurth R (2003) Genetic alterations of the long terminal repeat of an ecotropic porcine endogenous retrovirus during passage in human cells. Virology 314:125–133PubMedGoogle Scholar
  10. 10.
    Denner J, Young PR (2013) Koala retroviruses: characterization and impact on the life of koalas. Retrovirology 10:108PubMedPubMedCentralGoogle Scholar
  11. 11.
    DesGrosseillers L, Jolicoeur P (1984) The tandem direct repeats within the long terminal repeat of murine leukemia viruses are the primary determinant of their leukemogenic potential. J Virol 52:945–952Google Scholar
  12. 12.
    Fiebig U, Hartmann MG, Bannert N, Kurth R, Denner J (2006) Transspecies transmission of the endogenous koala retrovirus. J Virol 80:5651–5654PubMedPubMedCentralGoogle Scholar
  13. 13.
    Hanger JJ, Bromham LD, McKee JJ, O’Brien TM, Robinson WF (2000) The nucleotide sequence of koala (Phascolarctos cinereus) retrovirus: a novel type C endogenous virus related to Gibbon ape leukemia virus. J Virol 74:4264–4272PubMedPubMedCentralGoogle Scholar
  14. 14.
    Hobbs M, Pavasovic A, King AG, Prentis PJ, Eldridge MDB, Chen Z, Colgan DJ, Polkinghorne A, Wilkins MR, Flanagan C, Gillett A, Hanger J, Johnson RN, Timms P (2014) A transcriptome resource for the koala (Phascolarctos cinereus): insights into koala retrovirus transcription and sequence diversity. BMC Genomics 15:786PubMedPubMedCentralGoogle Scholar
  15. 15.
    Joyee AG, Thyagarajan SP, Reddy EV, Venkatesan C, Ganapathy M (2005) Genital chlamydial infection in STD patients: its relation to HIV infection. Indian J Med Microbiol 23:37–40PubMedGoogle Scholar
  16. 16.
    Kaptue L, Zekeng L, Djoumessi S, Monny-Lobe M, Nichols D, Debuysscher R (1991) HIV and chlamydia infections among prostitutes in Yaoundé, Cameroon. Genitourin Med 67(143–145):197Google Scholar
  17. 17.
    Karlas A, Irgang M, Votteler J, Specke V, Ozel M, Kurth R, Denner J (2010) Characterisation of a human cell-adapted porcine endogenous retrovirus PERV-A/C. Ann Transplant 15:45–54PubMedGoogle Scholar
  18. 18.
    Lieber MM (1975) Isolation from the Asian mouse Mus caroli of an endogenous type C virus related to infectious primate type C viruses. Proc Natl Acad Sci USA 72:2315–2319PubMedPubMedCentralGoogle Scholar
  19. 19.
    Martin J, Herniou E, Cook J, O’Neill RW, Tristem M (1999) Interclass transmission and phyletic host tracking in murine leukemia virus-related retroviruses. J Virol 73:2442–2449PubMedPubMedCentralGoogle Scholar
  20. 20.
    Mellors JW, Munoz A, Giorgi JV, Margolick JB, Tassoni CJ, Gupta P, Kingsley LA, Todd JA, Saah AJ, Detels R, Phair JP, Rinaldo CR Jr (1997) Plasma viral load and CD4 + lymphocytes as prognostic markers of HIV-1 infection. Ann Intern Med 126:946–954PubMedGoogle Scholar
  21. 21.
    Miyazawa T, Shojima T, Yoshikawa R, Ohata T (2011) Isolation of koala retroviruses from koalas in Japan. J Vet Med Sci 73:65–70PubMedGoogle Scholar
  22. 22.
    O’Dair HA, Hopper CD, Gruffydd-Jones TJ, Harbour DA, Waters L (1994) Clinical aspects of Chlamydia psittaci infection in cats infected with feline immunodeficiency virus. Vet Rec 134:365–368PubMedGoogle Scholar
  23. 23.
    Rohn J, Overbaugh J (1995) In vivo selection of long terminal repeat alterations in feline leukemia virus-induced thymic lymphomas. Virology 206:661–665PubMedGoogle Scholar
  24. 24.
    Scheef G, Fischer N, Krach U, Tonjes R (2001) The number of a U3 repeat box acting as an enhancer in long terminal repeats of polytropic replication-competent porcine endogenous retroviruses dynamically fluctuates during serial virus passages in human cells. J Virol 75:6933–6940PubMedPubMedCentralGoogle Scholar
  25. 25.
    Shojima T, Yoshikawa R, Hoshino S, Shimode S, Nakagawa S, Ohata T, Nakaoka R, Miyazawa T (2013) Identification of a novel subgroup of Koala retrovirus from Koalas in Japanese zoos. J Virol 87:9943–9948PubMedPubMedCentralGoogle Scholar
  26. 26.
    Simmons G, Clarke D, McKee J, Young P, Meers J (2014) Discovery of a novel retrovirus sequence in an Australian native rodent (Melomys burtoni): A putative link between gibbon ape leukemia virus and Koala retrovirus. PLoS One 9:e106954PubMedPubMedCentralGoogle Scholar
  27. 27.
    Simmons GS, Young PR, Hanger JJ, Jones K, Clarke D, McKee JJ, Meers J (2012) Prevalence of koala retrovirus in geographically diverse populations in Australia. Aust Vet J 90:404–409PubMedGoogle Scholar
  28. 28.
    Stewart H, Jarrett O, Hosie MJ, Willett BJ (2011) Are endogenous feline leukemia viruses really endogenous? Vet Immunol Immunopathol 143:325–331PubMedGoogle Scholar
  29. 29.
    Stoye JP, Coffin MCJM (1991) Virological events leading to spontaneous AKR thymomas. J Virol 65:1273–1285PubMedPubMedCentralGoogle Scholar
  30. 30.
    Tarlinton R, Meers J, Hanger J, Young P (2005) Real-time reverse transcriptase PCR for the endogenous koala retrovirus reveals an association between plasma viral load and neoplastic disease in koalas. J Gen Virol 86:783–787PubMedGoogle Scholar
  31. 31.
    Tarlinton RE, Meers J, Young PR (2006) Retroviral invasion of the koala genome. Nature 442:79–81PubMedGoogle Scholar
  32. 32.
    Viscott Comandini U, Massetti AP, Marchese R, Zaccarelli M, Vullo V, Delia S (1996) Chlamydia pneumoniae seroprevalence among HIV-1-infected and uninfected people with known HIV risk factor. AIDS 10:1543–1547PubMedGoogle Scholar
  33. 33.
    Wolgamot G, Miller A (1999) Replication of Mus dunni endogenous retrovirus depends on promoter activation followed by enhancer multimerization. J Virol 73:9803–9809PubMedPubMedCentralGoogle Scholar
  34. 34.
    Xu W, Stadler CK, Gorman K, Jensen N, Kim D, Zheng H, Tang S, Switzer WM, Pye GW, Eiden MV (2013) An exogenous retrovirus isolated from koalas with malignant neoplasias in a US zoo. Proc Natl Acad Sci USA 110:11547–11552PubMedPubMedCentralGoogle Scholar
  35. 35.
    Xu W, Gorman K, Santiago JC, Kluska K, Eiden MV (2015) Genetic diversity of koala retroviral envelopes. Viruses 7:1258–1270PubMedPubMedCentralGoogle Scholar

Copyright information

© Springer-Verlag Wien 2016

Authors and Affiliations

  1. 1.Robert Koch InstituteBerlinGermany

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