We characterized the genome of a densovirus, tentatively called human CSF-associated densovirus 1 (HuCSFDV1), in cerebrospinal fluid (CSF) from a human case of encephalitis with antibodies against the N-methyl D-aspartate receptor. The presence of the viral genome in CSF was independently confirmed. This virus, which is proposed to be a member of a new species in the genus Iteradensovirus of the subfamily Densovirinae, showed the typical two ORFs encoding nonstructural and structural proteins with low-level identities of 22 and 16 % to the closest known densovirus relative. No other eukaryotic viral sequences were detected using deep sequencing. The replication and pathogenicity in humans of this virus, which belongs to a viral subfamily whose members are only known to replicate in invertebrates, remain to be demonstrated. Alternative explanations for the detection of densovirus DNA in CSF are discussed.
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We acknowledge NHLBI Grant R01 HL105770 to E.L.D, the Blood Systems Research Institute, and NIH/NCATS Colorado CTSA Grant Number UL1 TR001082 for support. The contents are the authors’ sole responsibility and do not necessarily represent official NIH views.
This study was funded by NHLBI Grant R01 HL105770 and NIH/NCATS Colorado CTSA Grant Number UL1 TR001082.
Conflict of interest
All authors declare that there is no conflict of interest.
All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.
Informed consent was obtained from all individual participants included in the study.
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Phan, T.G., Messacar, K., Dominguez, S.R. et al. A new densovirus in cerebrospinal fluid from a case of anti-NMDA-receptor encephalitis. Arch Virol 161, 3231–3235 (2016). https://doi.org/10.1007/s00705-016-3002-9