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Reassortment among picobirnaviruses found in wolves


We conducted a viral metagenomics study in diarrheic free-ranging wolves in Portugal, revealing for the first time the presence of reassortant picobirnaviruses. These viruses shared identical capsid segments together with diverse RNA-dependent RNA polymerase segments. Even though causality between these picobirnaviruses and diarrhea could not be established, the study nonetheless confirms for the first time that wolves are a potential reservoir for picobirnaviruses, which might play a role as enteric pathogens.

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  1. Malik YS, Kumar N, Sharma K, Dhama K, Shabbir MZ, Ganesh B et al (2014) Epidemiology, phylogeny, and evolution of emerging enteric Picobirnaviruses of animal origin and their relationship to human strains. BioMed Res Int 2014:780752. doi:10.1155/2014/780752

    Article  PubMed  PubMed Central  Google Scholar 

  2. Pereira HG, Fialho AM, Flewett TH, Teixeira JM, Andrade ZP (1988) Novel viruses in human faeces. Lancet 2(8602):103–104

    CAS  Article  PubMed  Google Scholar 

  3. Ganesh B, Masachessi G, Mladenova Z (2014) Animal picobirnavirus. Virus Dis 25(2):223–238. doi:10.1007/s13337-014-0207-y

    Article  Google Scholar 

  4. Matthijnssens J, Heylen E, Zeller M, Rahman M, Lemey P, Van Ranst M (2010) Phylodynamic analyses of rotavirus genotypes G9 and G12 underscore their potential for swift global spread. Mol Biol Evol 27(10):2431–2436. doi:10.1093/molbev/msq137

    CAS  Article  PubMed  Google Scholar 

  5. Novel Swine-Origin Influenza AVIT, Dawood FS, Jain S, Finelli L, Shaw MW, Lindstrom S et al (2009) Emergence of a novel swine-origin influenza A (H1N1) virus in humans. N Engl J Med 360(25):2605–2615. doi:10.1056/NEJMoa0903810

    Article  Google Scholar 

  6. Banyai K, Martella V, Bogdan A, Forgach P, Jakab F, Meleg E et al (2008) Genogroup I picobirnaviruses in pigs: evidence for genetic diversity and relatedness to human strains. J Gen Virol 89(Pt 2):534–539. doi:10.1099/vir.0.83134-0

    CAS  Article  PubMed  Google Scholar 

  7. Álvares F (2004) Status and conservation of the Iberian wolf in Portugal. Wolf Print 20:4–6

    Google Scholar 

  8. Godinho R, Lopez-Bao JV, Castro D, Llaneza L, Lopes S, Silva P et al (2015) Real-time assessment of hybridization between wolves and dogs: combining noninvasive samples with ancestry informative markers. Mol Ecol Resour 15(2):317–328. doi:10.1111/1755-0998.12313

    CAS  Article  PubMed  Google Scholar 

  9. Conceição-Neto N, Zeller M, Lefrere H, De Bruyn P, Beller L, Deboutte W et al (2015) Modular approach to customise sample preparation procedures for viral metagenomics: a reproducible protocol for virome analysis. Sci Rep 5:16532. doi:10.1038/srep16532

    Article  Google Scholar 

  10. Martella V, Elia G, Buonavoglia C (2008) Canine distemper virus. The Veterinary clinics of North America Small animal practice 38(4):787–797, vii–viii. doi:10.1016/j.cvsm.2008.02.007

  11. Mech LD, Boitani L (2003) Wolves: behavior, ecology, and conservation. University of Chicago Press, Chicago

    Book  Google Scholar 

  12. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30(12):2725–2729. doi:10.1093/molbev/mst197

    CAS  Article  PubMed  PubMed Central  Google Scholar 

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We thank the ACHLI (Association for the Conservation of the Iberian Wolf Habitat) for funding wolf sampling, D. Castro (CTM/CIBIO) for genetic analysis of wolf scats, and D. Cadete, J. Bernardo and S. Pinto for conducting the scat survey. NCN was supported by the Institute for the Promotion of Innovation through Science and Technology in Flanders (IWT Vlaanderen). KCY was supported by the Interfaculty Council for Development Cooperation (IRO) from the KU Leuven. SR and RG were supported by ‘Fundacão para a Ciência e a Tecnologia (FCT, Portugal) (SFRH/BD/12291/2003 and IF/00564/2012, respectively).

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Correspondence to Jelle Matthijnssens.

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The authors declare no conflict of interest.

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Wolf monitoring and sampling was approved by the “National Authority for Nature Conservation (ICNF)” in Portugal.

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N. Conceição-Neto and J. R. Mesquita contributed equally to this work.

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Conceição-Neto, N., Mesquita, J.R., Zeller, M. et al. Reassortment among picobirnaviruses found in wolves. Arch Virol 161, 2859–2862 (2016).

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  • Enteric Pathogen
  • Canine Distemper Virus
  • Capsid Gene
  • Reassortment Event
  • Acute Watery Diarrhea