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Archives of Virology

, Volume 161, Issue 10, pp 2859–2862 | Cite as

Reassortment among picobirnaviruses found in wolves

  • Nádia Conceição-Neto
  • João Rodrigo Mesquita
  • Mark Zeller
  • Claude Kwe Yinda
  • Francisco Álvares
  • Sara Roque
  • Francisco Petrucci-Fonseca
  • Raquel Godinho
  • Elisabeth Heylen
  • Marc Van Ranst
  • Jelle Matthijnssens
Brief Report

Abstract

We conducted a viral metagenomics study in diarrheic free-ranging wolves in Portugal, revealing for the first time the presence of reassortant picobirnaviruses. These viruses shared identical capsid segments together with diverse RNA-dependent RNA polymerase segments. Even though causality between these picobirnaviruses and diarrhea could not be established, the study nonetheless confirms for the first time that wolves are a potential reservoir for picobirnaviruses, which might play a role as enteric pathogens.

Keywords

Enteric Pathogen Canine Distemper Virus Capsid Gene Reassortment Event Acute Watery Diarrhea 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

We thank the ACHLI (Association for the Conservation of the Iberian Wolf Habitat) for funding wolf sampling, D. Castro (CTM/CIBIO) for genetic analysis of wolf scats, and D. Cadete, J. Bernardo and S. Pinto for conducting the scat survey. NCN was supported by the Institute for the Promotion of Innovation through Science and Technology in Flanders (IWT Vlaanderen). KCY was supported by the Interfaculty Council for Development Cooperation (IRO) from the KU Leuven. SR and RG were supported by ‘Fundacão para a Ciência e a Tecnologia (FCT, Portugal) (SFRH/BD/12291/2003 and IF/00564/2012, respectively).

Compliance with ethical standards

Conflict of interest

The authors declare no conflict of interest.

Ethical approval

Wolf monitoring and sampling was approved by the “National Authority for Nature Conservation (ICNF)” in Portugal.

References

  1. 1.
    Malik YS, Kumar N, Sharma K, Dhama K, Shabbir MZ, Ganesh B et al (2014) Epidemiology, phylogeny, and evolution of emerging enteric Picobirnaviruses of animal origin and their relationship to human strains. BioMed Res Int 2014:780752. doi: 10.1155/2014/780752 CrossRefPubMedPubMedCentralGoogle Scholar
  2. 2.
    Pereira HG, Fialho AM, Flewett TH, Teixeira JM, Andrade ZP (1988) Novel viruses in human faeces. Lancet 2(8602):103–104CrossRefPubMedGoogle Scholar
  3. 3.
    Ganesh B, Masachessi G, Mladenova Z (2014) Animal picobirnavirus. Virus Dis 25(2):223–238. doi: 10.1007/s13337-014-0207-y CrossRefGoogle Scholar
  4. 4.
    Matthijnssens J, Heylen E, Zeller M, Rahman M, Lemey P, Van Ranst M (2010) Phylodynamic analyses of rotavirus genotypes G9 and G12 underscore their potential for swift global spread. Mol Biol Evol 27(10):2431–2436. doi: 10.1093/molbev/msq137 CrossRefPubMedGoogle Scholar
  5. 5.
    Novel Swine-Origin Influenza AVIT, Dawood FS, Jain S, Finelli L, Shaw MW, Lindstrom S et al (2009) Emergence of a novel swine-origin influenza A (H1N1) virus in humans. N Engl J Med 360(25):2605–2615. doi: 10.1056/NEJMoa0903810 CrossRefGoogle Scholar
  6. 6.
    Banyai K, Martella V, Bogdan A, Forgach P, Jakab F, Meleg E et al (2008) Genogroup I picobirnaviruses in pigs: evidence for genetic diversity and relatedness to human strains. J Gen Virol 89(Pt 2):534–539. doi: 10.1099/vir.0.83134-0 CrossRefPubMedGoogle Scholar
  7. 7.
    Álvares F (2004) Status and conservation of the Iberian wolf in Portugal. Wolf Print 20:4–6Google Scholar
  8. 8.
    Godinho R, Lopez-Bao JV, Castro D, Llaneza L, Lopes S, Silva P et al (2015) Real-time assessment of hybridization between wolves and dogs: combining noninvasive samples with ancestry informative markers. Mol Ecol Resour 15(2):317–328. doi: 10.1111/1755-0998.12313 CrossRefPubMedGoogle Scholar
  9. 9.
    Conceição-Neto N, Zeller M, Lefrere H, De Bruyn P, Beller L, Deboutte W et al (2015) Modular approach to customise sample preparation procedures for viral metagenomics: a reproducible protocol for virome analysis. Sci Rep 5:16532. doi: 10.1038/srep16532 CrossRefGoogle Scholar
  10. 10.
    Martella V, Elia G, Buonavoglia C (2008) Canine distemper virus. The Veterinary clinics of North America Small animal practice 38(4):787–797, vii–viii. doi: 10.1016/j.cvsm.2008.02.007
  11. 11.
    Mech LD, Boitani L (2003) Wolves: behavior, ecology, and conservation. University of Chicago Press, ChicagoCrossRefGoogle Scholar
  12. 12.
    Tamura K, Stecher G, Peterson D, Filipski A, Kumar S (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30(12):2725–2729. doi: 10.1093/molbev/mst197 CrossRefPubMedPubMedCentralGoogle Scholar

Copyright information

© Springer-Verlag Wien 2016

Authors and Affiliations

  • Nádia Conceição-Neto
    • 1
    • 2
  • João Rodrigo Mesquita
    • 3
    • 4
  • Mark Zeller
    • 1
  • Claude Kwe Yinda
    • 1
    • 2
  • Francisco Álvares
    • 4
  • Sara Roque
    • 5
    • 6
  • Francisco Petrucci-Fonseca
    • 5
    • 6
  • Raquel Godinho
    • 4
    • 7
  • Elisabeth Heylen
    • 1
  • Marc Van Ranst
    • 2
  • Jelle Matthijnssens
    • 1
  1. 1.Laboratory of Viral Metagenomics, Department of Microbiology and Immunology, Rega Institute for Medical ResearchKU Leuven, University of LeuvenLeuvenBelgium
  2. 2.Laboratory for Clinical Virology, Department of Microbiology and Immunology, Rega Institute for Medical ResearchKU Leuven, University of LeuvenLeuvenBelgium
  3. 3.Department of Biological Sciences, Faculty of PharmacyUniversity of PortoPortoPortugal
  4. 4.CIBIO/InBIO, Centro de Investigação em Biodiversidade e Recursos GenéticosUniversidade do PortoVairãoPortugal
  5. 5.cE3c, Centre for Ecology, Evolution and Environmental ChangesFaculdade de Ciências da Universidade de LisboaLisbonPortugal
  6. 6.Grupo Lobo, Departamento de Biologia AnimalFaculdade de Ciências da Universidade de LisboaLisbonPortugal
  7. 7.Departamento de Biologia, Faculdade de CiênciasUniversidade do PortoPortoPortugal

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