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Comparison of the immunogenicity of two inactivated recombinant rabies viruses overexpressing the glycoprotein

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Abstract

Two recombinant rabies viruses overexpressing their glycoprotein (G) were compared in this study, with the overexpressed G inserted between P and M genes (named LBNSE-PM-G), and between the G and L genes (named LBNSE-GL-G), respectively. LBNSE-PM-G produced more G protein and induced stronger apoptosis than LBNSE-GL-G in infected cells, while the amount of virion-incorporated G in LBNSE-PM-G was less than in LBNSE-GL-G. Mice immunized with inactivated LBNSE-PM-G produced lower titers of virus-neutralizing antibody, and this recombinant conferred worse protection than LBNSE-GL-G. Our results suggest that over expressed G gene inserted between G and L, but not between P and M, enhanced the immunogenicity when used as an inactivated rabies vaccine.

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Acknowledgments

This work was supported partially by the National Natural Science Foundation of China (31372419) and Huazhong Agricultural University Scientific & Technological Self-Innovation Foundation (2012RC009 and 2014JQ003) for LZ; National Natural Science Foundation of China (31330078) and Ministry of Agriculture of the People’s Republic of China (Special Fund for Agro-Scientific Research in the Public Interest, 201303042) for ZFF.

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    Authors and Affiliations

    1. State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, 430070, China

      Muhammad Tariq Navid, Yingying Li, Ming Zhou, Min Cui, Zhen F. Fu & Ling Zhao

    2. College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, China

      Muhammad Tariq Navid, Yingying Li, Ming Zhou, Min Cui, Zhen F. Fu & Ling Zhao

    3. Department of Pathology, University of Georgia, Athens, GA, 30602, USA

      Zhen F. Fu

    4. Hubei Provincial Key Laboratory for Applied Toxicology, Hubei Provincial Academy of Preventive Medicine, Wuhan, 430070, China

      Lijun Tang

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    1. Muhammad Tariq Navid
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    All procedures performed in studies involving animals were in accordance with the ethical standards approved by the Scientific Ethics Committee of Huazhong Agricultural University, China (HZAUMO-2015-016).

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    M. T. Navid and Y. Li contributed equally to this paper.

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    Navid, M.T., Li, Y., Zhou, M. et al. Comparison of the immunogenicity of two inactivated recombinant rabies viruses overexpressing the glycoprotein. Arch Virol 161, 2863–2870 (2016). https://doi.org/10.1007/s00705-016-2967-8

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    • DOI: https://doi.org/10.1007/s00705-016-2967-8

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