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Lytic bacteriophage PM16 specific for Proteus mirabilis: a novel member of the genus Phikmvvirus

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Abstract

Lytic Proteus phage PM16, isolated from human faeces, is a novel virus that is specific for Proteus mirabilis cells. Bacteriophage PM16 is characterized by high stability, a short latency period, large burst size and the occurrence of low phage resistance. Phage PM16 was classified as a member of the genus Phikmvvirus on the basis of genome organization, gene synteny, and protein sequences similarities. Within the genus Phikmvvirus, phage PM16 is grouped with Vibrio phage VP93, Pantoea phage LIMElight, Acinetobacter phage Petty, Enterobacter phage phiKDA1, and KP34-like bacteriophages. An investigation of the phage-cell interaction demonstrated that phage PM16 attached to the cell surface, not to the bacterial flagella. The study of P. mirabilis mutant cells obtained during the phage-resistant bacterial cell assay that were resistant to phage PM16 re-infection revealed a non-swarming phenotype, changes in membrane characteristics, and the absence of flagella. Presumably, the resistance of non-swarming P. mirabilis cells to phage PM16 re-infection is determined by changes in membrane macromolecular composition and is associated with the absence of flagella and a non-swarming phenotype.

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Acknowledgments

Funding: This research was financially supported by the Program of Presidium of the Russian Academy of Sciences “Basic researches for development of medical technologies” (Grant No. 2014-155) and by Ministry of Education and Science of the Russian Federation (Grant No. VI.55.1.1 “Genomics of bacterial and viral communities”).

The authors wish to thank I.V. Saranina, G.B. Kaverina and E.P. Panferova for excellent technical support. They also thank doctors V.V. Anishenko and S.A. Semenov from Railway Clinical Hospital (Novosibirsk, Russia) for providing clinical samples.

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  1. Institute of Chemical Biology and Fundamental Medicine, Lavrentieva Ave., 8, Novosibirsk, Russia

    V. Morozova, Yu. Kozlova, E. Shedko, A. Kurilshikov, I. Babkin, A. Tupikin, A. Yunusova, A. Chernonosov, I. Baykov, M. Kabilov, E. Ryabchikova, V. Vlassov & N. Tikunova

  2. Limnological Institute of SB RAS, Ulan-Batorskaya Str., 3, Irkutsk, Russia

    I. Кondratov

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  1. V. Morozova
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All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards. The research was approved by the Local Ethical Committee of the Center of New Medical Technology, Novosibirsk and informed consent from the patient was obtained.

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Morozova, V., Kozlova, Y., Shedko, E. et al. Lytic bacteriophage PM16 specific for Proteus mirabilis: a novel member of the genus Phikmvvirus . Arch Virol 161, 2457–2472 (2016). https://doi.org/10.1007/s00705-016-2944-2

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