A novel putative ampelovirus was detected in grapevines that showed typical leafroll symptoms and was tentatively named grapevine leafroll-associated virus (GLRaV)-13 following the series of numbering of other GLRaVs. The complete genome of GLRaV-13 comprised 17,608 nt and contained eleven putative open reading frames, showing genetic features similar to those of viruses belonging to subgroup I of genus Ampelovirus. Phylogenetic trees based on the RNA-dependent RNA polymerase, heat shock protein 70 homolog, and coat protein showed that GLRaV-13 had the closest, but still distant, relationship to GLRaV-1 in the subgroup I cluster.
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The authors thank Drs. Sato and Mitani for the grapevine samples, and Dr. Nakano for the instruction of the DAS-ELISA.
This study was funded by NARO, Japan.
Conflict of interest
The authors declare no conflict of interest associated with this manuscript.
This article does not contain any studies with animals performed by any of the authors.
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Fig. S1 Phylogenetic trees of (a) HSP70h, (b) RdRp, and (c) CP amino acid sequences illustrating the relationship of grapevine leafroll-associated virus 13 (shown as GLRaVAk, underlined) to members of the family Closteroviridae, constructed using the maximum-likelihood algorithm and 100 bootstrap replications. (a) The HSP70 sequence from Arabidopsis thaliana (NP_187864), (b) the RdRp sequence of cucumber mosaic virus (NP_049324), and (c) the CP sequence of bean calico mosaic virus (NP_612594) were used as outgroups. Amino acid sequences were aligned and analyzed using CLUSTAL W , and MEGA software version 6  was used to construct the trees. Bootstrap values are shown at the nodes. The scale represents amino acid substitutions per site. Accession numbers of their nucleotide sequences and their products follow the hyphens. Vertical bars indicate the four genera. Subgroup I and II of the genus Ampelovirus  are boxed with a solid line and a broken line, respectively (PDF 328 kb)
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Ito, T., Nakaune, R. Molecular characterization of a novel putative ampelovirus tentatively named grapevine leafroll-associated virus 13. Arch Virol 161, 2555–2559 (2016). https://doi.org/10.1007/s00705-016-2914-8