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Pittosporum cryptic virus 1: genome sequence completion using next-generation sequencing

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Abstract

Next-generation sequencing (NGS) was applied to dsRNAs extracted from an Italian pittosporum plant infected with pittosporum cryptic virus 1 (PiCV1). NGS allowed assembly of the full genome sequence of PiCV1, comprising dsRNA1 (1.9 kbp) and dsRNA2 (1.5 kbp), which encode the RNA-dependent RNA polymerase and capsid protein genes, respectively. Phylogenetic and sequence analyses confirmed that PiCV1 is a new member of the genus Deltapartitivirus, family Partiviridae. From the same plant, NSG also permitted assembly of the complete genome sequence of eggplant mottled dwarf virus (EMDV), which shared 86 % to 98 % nucleotide sequence identity with complete and partial sequences (ca 6750 nt) of other known EMDV isolates with sequences available in the GenBank database.

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Authors and Affiliations

  1. Istituto Agronomico Mediterraneo di Bari, Via Ceglie 9, 70010, Valenzano (Bari), Italy

    Toufic Elbeaino, Raied Abou Kubaa & Michele Digiaro

  2. Department of Plant Protection, Faculty of Agriculture, University of Çanakkale Onsekiz Mart, 17020, Canakkale, Turkey

    Hasan Tuna Tuzlali

Authors
  1. Toufic Elbeaino
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  2. Raied Abou Kubaa
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  3. Hasan Tuna Tuzlali
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  4. Michele Digiaro
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Corresponding author

Correspondence to Toufic Elbeaino.

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The authors declare that they have no conflict of interest, and no experiments on animals were conducted.

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Elbeaino, T., Kubaa, R.A., Tuzlali, H.T. et al. Pittosporum cryptic virus 1: genome sequence completion using next-generation sequencing. Arch Virol 161, 2039–2042 (2016). https://doi.org/10.1007/s00705-016-2860-5

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  • DOI: https://doi.org/10.1007/s00705-016-2860-5

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