Genomic, pathogenic, and antigenic comparisons of Getah virus strains isolated in 1978 and 2014 in Japan
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A Getah virus strain isolated during an outbreak in racehorses in Japan in 2014 (14-I-605) was compared with the vaccine strain isolated in 1978 (MI-110). A comparison of the genome sequences of these strains revealed seven amino acid substitutions in non-structural protein 3, and one or two substitutions in each of other non-structural proteins. In contrast, the structural proteins were highly conserved (99.8–99.9 % amino acid sequence identity). Horse antisera raised against the MI-110 strain showed similar virus-neutralization titers against both MI-110 and 14-I-605 strains (512 and 256, respectively). Therefore, antigenic mutation was probably not a direct cause of the outbreak that occurred in 2014.
KeywordsReverse Transcription Polymerase Chain Reaction Vero Cell Training Center Nonstructural Protein nsP3 Protein
We are grateful to Dr. Chihiro Yoshihara (RIKEN Brain Science Institute, Wako, Japan) and Mr. Akira Kokubun, Ms. Akiko Suganuma, Ms. Kazue Arakawa, and Ms. Kaoru Makabe (Equine Research Institute, Japan Racing Association) for providing technical assistance.
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Conflict of interest
The authors declare that they have no conflicts of interest.
- 3.Griffin DE (2013) Alphaviruses. In: Knipe DM, Howley PM (eds) Fields virology. Lippincott Williams and Wilkins, Philadelphia, pp 651–686Google Scholar
- 8.Tajima S, Kotaki A, Yagasaki K, Taniwaki T, Moi ML, Nakayama E, Saijo M, Kurane I, Takasaki T (2014) Identification and amplification of Japanese encephalitis virus and Getah virus propagated from a single porcine serum sample: a case of coinfection. Arch Virol 159:2969–2975CrossRefPubMedGoogle Scholar