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Archives of Virology

, Volume 161, Issue 6, pp 1691–1695 | Cite as

Genomic, pathogenic, and antigenic comparisons of Getah virus strains isolated in 1978 and 2014 in Japan

  • Manabu Nemoto
  • Hiroshi Bannai
  • Koji Tsujimura
  • Takashi Yamanaka
  • Takashi Kondo
Brief Report

Abstract

A Getah virus strain isolated during an outbreak in racehorses in Japan in 2014 (14-I-605) was compared with the vaccine strain isolated in 1978 (MI-110). A comparison of the genome sequences of these strains revealed seven amino acid substitutions in non-structural protein 3, and one or two substitutions in each of other non-structural proteins. In contrast, the structural proteins were highly conserved (99.8–99.9 % amino acid sequence identity). Horse antisera raised against the MI-110 strain showed similar virus-neutralization titers against both MI-110 and 14-I-605 strains (512 and 256, respectively). Therefore, antigenic mutation was probably not a direct cause of the outbreak that occurred in 2014.

Keywords

Reverse Transcription Polymerase Chain Reaction Vero Cell Training Center Nonstructural Protein nsP3 Protein 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

We are grateful to Dr. Chihiro Yoshihara (RIKEN Brain Science Institute, Wako, Japan) and Mr. Akira Kokubun, Ms. Akiko Suganuma, Ms. Kazue Arakawa, and Ms. Kaoru Makabe (Equine Research Institute, Japan Racing Association) for providing technical assistance.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflicts of interest.

Supplementary material

705_2016_2840_MOESM1_ESM.xls (35 kb)
Supplementary material 1 (XLS 35 kb)
705_2016_2840_MOESM2_ESM.doc (40 kb)
Supplementary material 2 (DOC 39 kb)
705_2016_2840_MOESM3_ESM.doc (48 kb)
Supplementary material 3 (DOC 48 kb)

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Copyright information

© Springer-Verlag Wien 2016

Authors and Affiliations

  1. 1.Equine Research Institute, Japan Racing AssociationShimotsukeJapan

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