Abstract
In this study of three grapevine leafroll-associated virus 3 (GLRaV-3) genetic variants in two grapevine rootstock hosts, GLRaV-3 detection was shown to be affected by the virus distribution, titre, and the genetic variant. Group VI and NZ2 GLRaV-3 variants had reduced detectability compared with the group I variant. Differences in the genomic and subgenomic RNA (sgRNA) expression levels, and differences in the level of expression between the genetic variants were also observed. The observed differences in virus titre and sgRNA expression levels suggest differences in plant-virus interactions by the various GLRaV-3 genetic variants.
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Acknowledgments
The authors would like to thank Arnaud Blouin, Dr. Sarah Jane Cowell, and Dr. Mahmoud Khalifa for their comments on the manuscript. This research was supported by New Zealand Winegrowers, Corbans Viticulture Ltd., The New Zealand Institute for Plant & Food Research Limited, and the University of Auckland. During this research, K. M. Chooi was supported by a Top Achiever Doctoral Scholarship from the New Zealand Tertiary Education Commission, and subsequently by a Post-Doctoral Fellowship from The Agricultural and Marketing Research and Development Trust.
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This study was funded by the New Zealand Tertiary Education Commission Bright Futures Scheme Top Achiever Doctoral Scholarship, New Zealand Winegrowers (Grant no. NZW12-118PFR), and The Agricultural and Marketing Research and Development Trust Post-Doctoral Fellowship (Grant no. P14002).
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Chooi, K.M., Cohen, D. & Pearson, M.N. Differential distribution and titre of selected grapevine leafroll-associated virus 3 genetic variants within grapevine rootstocks. Arch Virol 161, 1371–1375 (2016). https://doi.org/10.1007/s00705-016-2791-1
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DOI: https://doi.org/10.1007/s00705-016-2791-1