Skip to main content

Advertisement

SpringerLink
Log in

Genetic characterization of influenza A (H7N6) virus isolated from a live-bird market in Thailand

  • Brief Report
  • Published:
Archives of Virology Aims and scope Submit manuscript

Abstract

A one-year influenza A virus (IAV) monitoring program was conducted in a live-bird market (LBM) in Thailand. Using one-step real-time RT-PCR (rRT-PCR), 2.39 % of live birds were found to be IAV positive. Twenty viruses could be identified as IAV subtype H7N6. Eight IAV-H7N6 viruses were subjected to whole-genome sequencing and genetic characterization. Phylogenetic analysis showed that the HA gene of Thai H7N6 is grouped with those of the H7 Eurasian viruses. The NA gene is closely related to those of the N6 Eurasian viruses. This is the first report of IAV subtype H7N6 in Thailand.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

References

  1. Abdelwhab EM, Selim AA, Arafa A, Galal S, Kilany WH, Hassan MK, Aly MM, Hafez MH (2010) Circulation of avian influenza H5N1 in live bird markets in Egypt. Avian Dis 54:911–914

    Article  CAS  PubMed  Google Scholar 

  2. Amonsin A, Choatrakol C, Lapkuntod J, Tantilertcharoen R, Thanawongnuwech R, Suradhat S, Suwannakarn K, Theamboonlers A, Poovorawan Y (2008) Influenza virus (H5N1) in live bird markets and food markets, Thailand. Emerg Infect Dis 14:1739–1742

    Article  PubMed  PubMed Central  Google Scholar 

  3. Banks J, Plowright L (2003) Additional glycosylation at the receptor binding site of the hemagglutinin (HA) for H5 and H7 viruses may be an adaptation to poultry hosts, but does it influence pathogenicity? Avian Dis 47:942–950

    Article  CAS  PubMed  Google Scholar 

  4. Butt KM, Smith GJ, Chen H, Zhang LJ, Leung YH, Xu KM, Lim W, Webster RG, Yuen KY, Peiris JS, Guan Y (2005) Human infection with an avian H9N2 influenza A virus in Hong Kong in 2003. J Clin Microbiol 43:5760–5767

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  5. Capua I, Mutinelli F, Marangon S, Alexander DJ (2000) H7N1 avian influenza in Italy (1999 to 2000) in intensively reared chickens and turkeys. Avian Pathol J WVPA 29:537–543

    Article  CAS  Google Scholar 

  6. Cardona C, Yee K, Carpenter T (2009) Are live bird markets reservoirs of avian influenza? Poult Sci 88:856–859

    Article  CAS  PubMed  Google Scholar 

  7. Chen Y, Liang W, Yang S, Wu N, Gao H, Sheng J, Yao H, Wo J, Fang Q, Cui D, Li Y, Yao X, Zhang Y, Wu H, Zheng S, Diao H, Xia S, Zhang Y, Chan KH, Tsoi HW, Teng JL, Song W, Wang P, Lau SY, Zheng M, Chan JF, To KK, Chen H, Li L, Yuen KY (2013) Human infections with the emerging avian influenza A H7N9 virus from wet market poultry: clinical analysis and characterisation of viral genome. Lancet 381:1916–1925

    Article  CAS  PubMed  Google Scholar 

  8. Connor RJ, Kawaoka Y, Webster RG, Paulson JC (1994) Receptor specificity in human, avian, and equine H2 and H3 influenza virus isolates. Virology 205:17–23

    Article  CAS  PubMed  Google Scholar 

  9. Cowling BJ, Jin L, Lau EH, Liao Q, Wu P, Jiang H, Tsang TK, Zheng J, Fang VJ, Chang Z, Ni MY, Zhang Q, Ip DK, Yu J, Li Y, Wang L, Tu W, Meng L, Wu JT, Luo H, Li Q, Shu Y, Li Z, Feng Z, Yang W, Wang Y, Leung GM, Yu H (2013) Comparative epidemiology of human infections with avian influenza A H7N9 and H5N1 viruses in China: a population-based study of laboratory-confirmed cases. Lancet 382:129–137

    Article  PubMed  PubMed Central  Google Scholar 

  10. Han J, Jin M, Zhang P, Liu J, Wang L, Wen D, Wu X, Liu G, Zou Y, Lv X, Dong X, Shao B, Gu S, Zhou D, Leng Q, Zhang C, Lan K (2013) Epidemiological link between exposure to poultry and all influenza A (H7N9) confirmed cases in Huzhou city, China, March to May 2013. Eur Surveill Bull Eur Mal Transm Eur Comm Dis Bull 18:1–6

    CAS  Google Scholar 

  11. Lowen AC, Mubareka S, Steel J, Palese P (2007) Influenza virus transmission is dependent on relative humidity and temperature. PLoS Pathog 3:1470–1476

    Article  CAS  PubMed  Google Scholar 

  12. Moon HJ, Song MS, Cruz DJ, Park KJ, Pascua PN, Lee JH, Baek YH, Choi DH, Choi YK, Kim CJ (2010) Active reassortment of H9 influenza viruses between wild birds and live-poultry markets in Korea. Arch Virol 155:229–241

    Article  CAS  PubMed  Google Scholar 

  13. OIE (2008) Avian influenza, 6th edn. World Organisation for Animal Health, Paris

    Google Scholar 

  14. OIE (2012) Avian influenza, 7th edn. World Organisation for Animal Health, Paris

    Google Scholar 

  15. Perdue ML, Suarez DL (2000) Structural features of the avian influenza virus hemagglutinin that influence virulence. Vet Microbiol 74:77–86

    Article  CAS  PubMed  Google Scholar 

  16. Santhia K, Ramy A, Jayaningsih P, Samaan G, Putra AA, Dibia N, Sulaimin C, Joni G, Leung CY, Sriyal J, Peiris M, Wandra T, Kandun N (2009) Avian influenza A H5N1 infections in Bali Province, Indonesia: a behavioral, virological and seroepidemiological study. Influenza Other Respir Viruses 3:81–89

    Article  PubMed  PubMed Central  Google Scholar 

  17. Sreta D, Tantawet S, Na Ayudhya SN, Thontiravong A, Wongphatcharachai M, Lapkuntod J, Bunpapong N, Tuanudom R, Suradhat S, Vimolket L, Poovorawan Y, Thanawongnuwech R, Amonsin A, Kitikoon P (2010) Pandemic (H1N1) 2009 virus on commercial swine farm, Thailand. Emerg Infect Dis 16:1587–1590

    Article  PubMed  PubMed Central  Google Scholar 

  18. Su C, Chen S, Liu X, Xu J, Huang K, Liu K, Huang J, Peng D, Liu X (2013) Genome sequence of a novel H10N9 avian influenza virus isolated from chickens in a live poultry market in eastern China. Genome Announc 1:e00386-1–e00386-13

    Google Scholar 

  19. Swayne DE (2007) Understanding the complex pathobiology of high pathogenicity avian influenza viruses in birds. Avian Dis 51:242–249

    Article  PubMed  Google Scholar 

  20. Tsukamoto K, Ashizawa H, Nakanishi K, Kaji N, Suzuki K, Okamatsu M, Yamaguchi S, Mase M (2008) Subtyping of avian influenza viruses H1 to H15 on the basis of hemagglutinin genes by PCR assay and molecular determination of pathogenic potential. J Clin Microbiol 46:3048–3055

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  21. Tsukamoto K, Ashizawa T, Nakanishi K, Kaji N, Suzuki K, Shishido M, Okamatsu M, Mase M (2009) Use of reverse transcriptase PCR to subtype N1 to N9 neuraminidase genes of avian influenza viruses. J Clin Microbiol 47:2301–2303

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  22. VanDalen KK, Anderson TD, Killian ML, Pedersen JC, Franklin AB, Piaggio AJ (2008) Increased detection of influenza A H16 in the United States. Arch Virol 153:1981–1983

    Article  CAS  PubMed  Google Scholar 

  23. Wan XF, Dong L, Lan Y, Long LP, Xu C, Zou S, Li Z, Wen L, Cai Z, Wang W, Li X, Yuan F, Sui H, Zhang Y, Dong J, Sun S, Gao Y, Wang M, Bai T, Yang L, Li D, Yang W, Yu H, Wang S, Feng Z, Wang Y, Guo Y, Webby RJ, Shu Y (2011) Indications that live poultry markets are a major source of human H5N1 influenza virus infection in China. J Virol 85:13432–13438

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  24. Webster RG, Bean WJ, Gorman OT, Chambers TM, Kawaoka Y (1992) Evolution and ecology of influenza A viruses. Microbiol Rev 56:152–179

    CAS  PubMed  PubMed Central  Google Scholar 

  25. Wisedchanwet T, Wongpatcharachai M, Boonyapisitsopa S, Bunpapong N, Jairak W, Kitikoon P, Sasipreeyajun J, Amonsin A (2011) Influenza A virus surveillance in live-bird markets: first report of influenza A virus subtype H4N6, H4N9, and H10N3 in Thailand. Avian Dis 55:593–602

    Article  PubMed  Google Scholar 

  26. Wongphatcharachai M, Wisedchanwet T, Lapkuntod J, Nonthabenjawan N, Jairak W, Amonsin A (2012) Genetic characterization of influenza A virus subtype H12N1 isolated from a watercock and lesser whistling ducks in Thailand. Arch Virol 157:1123–1130

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

We would like to acknowledge Chulalongkorn University for its financial support to the Center of Excellence for Emerging and Re-emerging Infectious Diseases in Animals. We would like to thank the Thailand Research Fund for its financial support to the TRF Senior Scholar to AA (RTA5780006).

Author information

Authors and Affiliations

  1. Department of Veterinary Public Health, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, 10330, Thailand

    Waleemas Jairak, Supassama Chaiyawong, Nutthawan Nonthabenjawan, Ratanaporn Tangwangvivat & Alongkorn Amonsin

  2. Emerging and Re-emerging Infectious Diseases in Animals, Center of Excellence, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, 10330, Thailand

    Waleemas Jairak, Supanat Boonyapisitsopa, Supassama Chaiyawong, Nutthawan Nonthabenjawan, Ratanaporn Tangwangvivat, Napawan Bunpapong & Alongkorn Amonsin

Authors
  1. Waleemas Jairak
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Supanat Boonyapisitsopa
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Supassama Chaiyawong
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Nutthawan Nonthabenjawan
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Ratanaporn Tangwangvivat
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Napawan Bunpapong
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Alongkorn Amonsin
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Alongkorn Amonsin.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary material 1 (DOCX 16 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Jairak, W., Boonyapisitsopa, S., Chaiyawong, S. et al. Genetic characterization of influenza A (H7N6) virus isolated from a live-bird market in Thailand. Arch Virol 161, 1315–1322 (2016). https://doi.org/10.1007/s00705-016-2759-1

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00705-016-2759-1

Keywords

Search

Navigation