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Molecular characterization of a novel capillovirus from red currant

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Abstract

The complete nucleotide sequence of a novel virus from red currant, provisionally named currant virus A (CuVA), was determined. The genome is 7925 nucleotides long and has a 3′-poly(A) tail. The genome organization with two overlapping open reading frames is similar to that of capilloviruses, but the CuVA genome is about 600 nucleotides longer than that of the longest known capillovirus, cherry virus A. The RNA is predicted to encode a polyprotein with domains of methyltransferase, 2OG-Fe(II) oxygenase, papain-like protease, RNA helicase, RdRp, and capsid protein. Phylogenetic analysis confirms that CuVA is a new and distinct member of the genus Capillovirus.

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References

  1. Adams MJ, Candresse T, Hammond J, Kreuze JF, Martelli GP, Namba S, Pearson MN, Ryu KH, Saldarelli P, Yoshikawa N (2012) Betaflexiviridae. In: King AMQ, Adams MJ, Carstens EB, Lefkowitz EJ (eds) Virus taxonomy, the Ninth Report of the International Committee on Taxonomy of Viruses. Academic Press, London, pp 920–941

    Google Scholar 

  2. Massard S, Jijakli MH, Kummert J (2011) Apple stem grooving virus. In: Hadidi A, Barba M, Candresse T, Jelkmann W (eds) Virus and virus-like diseases of pome and stone fruits. APS Press, St. Paul, pp 29–33

    Google Scholar 

  3. Chen S, Ye T, Hao L, Chen H, Wang S, Fan Z, Guo L, Zhou T (2014) Infection of apple by Apple stem grooving virus leads to extensive alterations in gene expression patterns but no disease symptoms. PLoS One 9(4):e95239

    Article  PubMed  PubMed Central  Google Scholar 

  4. Marais A, Candresse T, Svanella-Dumas L, Jelkmann W (2012) Cherry virus A. In: Hadidi A, Barba M, Candresse T, Jelkmann W (eds) Virus and virus-like diseases of pome and stone fruits. APS Press, St. Paul, pp 147–150

    Google Scholar 

  5. Přibylová J, Špak J, Kubelková D (2002) Mixed infection of blackcurrant (Ribes nigrum L.) plants with blackcurrant reversion associated virus and rhabdovirus-like particles with symptoms of black currant. Acta Virol 46:253–256

    PubMed  Google Scholar 

  6. McGavin WJ, McMenemy LS, MacFarlane SA (2010) The complete sequence of a UK strain of black raspberry necrosis virus. Arch Virol 155:1897–1899

    Article  CAS  PubMed  Google Scholar 

  7. Besse S, Gugerli P, Ramel ME, Balmelli C (2010) Characterisation of mixed virus infections in Ribes species in Switzerland. Julius-Kuehn Archiv 427:214–219

    Google Scholar 

  8. Martin RR, MacFarlane S, Sabanadzovic S, Quito D, Poudel B, Tzanetakis IE (2013) Viruses and virus diseases of Rubus. Plant Dis 97:168–182

    Article  Google Scholar 

  9. De Paulo JJ, Powel CA (1995) Extraction of double-stranded RNA from plant tissue without the use of organic solvents. Plant Dis 79:246–248

    Article  Google Scholar 

  10. Lütcke HA, Chow KC, Mickel FS, Moss KA, Kern HF, Scheele GA (1987) Selection of AUG initiation codons in plants and animals. EMBO J 6:43–48

    PubMed  PubMed Central  Google Scholar 

  11. Liebenberg A, Moury B, Sabath N, Hell R, Kappis A, Jarausch W, Wetzel T (2012) Molecular evolution of the genomic RNA of apple stem grooving capillovirus. J Mol Evol 75:92–101

    Article  CAS  PubMed  Google Scholar 

  12. Hirata H, Yamaji Y, Komatsu K, Kagiwada S, Oshima K, Okano Y, Takahashi S, Ugaki M, Namba S (2010) Pseudo-polyprotein translated from the full-length ORF1 of capillovirus is important for pathogenicity, but a truncated ORF1 protein without variable and CP regions is sufficient for replication. Virus Res 152:1–9

    Article  CAS  PubMed  Google Scholar 

  13. Wylie S, Jones M (2011) Hardenbergia virus A, a novel member of the family Betaflexiviridae from a wild legume in Southwest Australia. Arch Virol 156:1245–1250

    Article  CAS  PubMed  Google Scholar 

  14. Tatineni S, Afunian MR, Gowda S, Hilf ME, Bar-Josef M, Dawson WO (2009) Characterization of the 5′- and 3′-terminal subgenomic RNAs produced by a capillovirus: Evidence for a CP subgenomic RNA. Virology 385:521–528

    Article  CAS  PubMed  Google Scholar 

  15. Shim H, Min Y, Hong S, Kwon M, Kim D, Kim H, Choi Y, Lee S, Yang J (2004) Nucleotide sequences of a Korean isolate of Apple stem grooving virus associated with black necrotic leaf spot disease on pear (Pyrus pyrifolia). Mol. Cells 18:192–199

    CAS  PubMed  Google Scholar 

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Acknowledgments

This work was performed with institutional support RVO:60077344 from the Institute of Plant Molecular Biology, Biology Centre of the Czech Academy of Sciences. The authors thank J. Sedlák and F. Paprštein for access to the germplasm collection at the Research and Breeding Institute of Pomology, Holovousy, Czech Republic.

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Authors and Affiliations

  1. Department of Plant Virology, Institute of Plant Molecular Biology, Biology Centre of the Czech Academy of Sciences, v.v.i., Branišovská 31, 370 05, České Budějovice, Czech Republic

    Karel Petrzik, Jaroslava Přibylová, Igor Koloniuk & Josef Špak

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  1. Karel Petrzik
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  2. Jaroslava Přibylová
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  3. Igor Koloniuk
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  4. Josef Špak
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Correspondence to Karel Petrzik.

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Petrzik, K., Přibylová, J., Koloniuk, I. et al. Molecular characterization of a novel capillovirus from red currant. Arch Virol 161, 1083–1086 (2016). https://doi.org/10.1007/s00705-016-2752-8

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  • DOI: https://doi.org/10.1007/s00705-016-2752-8

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