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Complete genome sequence of a novel zantedeschia mild mosaic virus isolate: the first report from Australia and from Alocasia sp.

Archives of Virology volume 161, pages 1079–1082 (2016)Cite this article

Abstract

The complete genome of an Australian isolate of zantedeschia mild mosaic virus (ZaMMV) causing mosaic symptoms on Alocasia sp. (designated ZaMMV-AU) was cloned and sequenced. The genome comprises 9942 nucleotides (excluding the poly-A tail) and encodes a polyprotein of 3167 amino acids. The sequence is most closely related to a previously reported ZaMMV isolate from Taiwan (ZaMMV-TW), with 82 and 86 % identity at the nucleotide and amino acid level, respectively. Unlike the amino acid sequence of ZaMMV-TW, however, ZaMMV-AU does not contain a polyglutamine stretch at the N-terminus of the coat-protein-coding region upstream of the DAG motif. This is the first report of ZaMMV from Australia and from Alocasia sp.

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References

  1. Huang CH, Chang YC (2005) Identification and molecular characterization of Zantedeschia mild mosaic virus, a new calla lily-infecting potyvirus. Arch Virol 150:1221–1230

    Article  CAS  PubMed  Google Scholar 

  2. Huang CH, Hu WC, Yang TC, Chang YC (2007) Zantedeschia mild mosaic virus, a new widespread virus in calla lily, detected by ELISA, dot-blot hybridization and IC-RT-PCR. Plant Pathol 56:183–189

    Article  CAS  Google Scholar 

  3. Rizzo D, Panattoni A, Stefani L, Paoli M, Nesi B, Lazzereschi S, Vanarelli S, Farina P, Della Bartola M, Materazzi A, Luvisi A (2015) First report of Zantedeschia mild mosaic virus on Zantedeschia aethiopica (L) Spreng in Italy. J Plant Pathol 97:1–2

    Google Scholar 

  4. Valderrama-Cháirez ML, Cruz-Hernández A, Paredes-López O (2002) Isolation of functional RNA from cactus fruit. Plant Mol Biol Rep 20:279–286

    Article  Google Scholar 

  5. Ha C, Revill P, Harding RM, Vu M, Dale JL (2008) Identification and sequence analysis of potyviruses infecting crops in Vietnam. Arch Virol 153:45–60

    Article  CAS  PubMed  Google Scholar 

  6. Ha C, Coombs S, Revill P, Harding RM, Vu M, Dale JL (2008) Design and application of two novel degenerate primer pairs for the detection and complete genomic characterization of potyviruses. Arch Virol 153:25–36

    Article  CAS  PubMed  Google Scholar 

  7. Yamamoto H, Fuji S (2008) Rapid determination of the nucleotide sequences of potyviral coat protein genes using semi-nested RT-PCR with universal primers. J Gen Plant Pathol 74:97–100

    Article  CAS  Google Scholar 

  8. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30:2725–2729

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  9. Adams MJ, Zerbini FM, French R, Rabenstein F, Stenger DC, Valkonen JPT (2012) Family Potyviridae. In: King AMQ, Lefkowitz E, Adams MJ, Carstens EB (eds) Virus taxonomy: ninth report of the International Committee on Taxonomy of Viruses, London, pp 1069–1089

  10. Adams MJ, Antoniw JF, Fauquet CM (2005) Molecular criteria for genus and species discrimination within the family Potyviridae. Arch Virol 150:459–479

    Article  CAS  PubMed  Google Scholar 

  11. Adams MJ, Antoniw JF, Beaudoin F (2005) Overview and analysis of the polyprotein cleavage sites in the family Potyviridae. Mol Plant Pathol 6:471–487

    Article  CAS  PubMed  Google Scholar 

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Acknowledgments

DK is the recipient of an Australia Awards Scholarship.

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Authors and Affiliations

  1. Centre for Tropical Crops and Biocommodities, Queensland University of Technology (QUT), Brisbane, 4001, Australia

    Dawit B. Kidanemariam, Amit C. Sukal, James L. Dale, Anthony P. James & Robert M. Harding

  2. Holetta National Agricultural Biotechnology Research Center, Ethiopian Institute of Agricultural Research, P.O. Box 2003, Addis Ababa, Ethiopia

    Dawit B. Kidanemariam & Adane D. Abraham

  3. Biosciences for Eastern and Central Africa, International Livestock Research Institute (BecA-ILRI Hub), P.O. Box 30709, Nairobi, Kenya

    Timothy A. Holton

Authors
  1. Dawit B. Kidanemariam
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  2. Adane D. Abraham
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  3. Amit C. Sukal
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  4. Timothy A. Holton
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  5. James L. Dale
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  6. Anthony P. James
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  7. Robert M. Harding
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Correspondence to Robert M. Harding.

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Kidanemariam, D.B., Abraham, A.D., Sukal, A.C. et al. Complete genome sequence of a novel zantedeschia mild mosaic virus isolate: the first report from Australia and from Alocasia sp.. Arch Virol 161, 1079–1082 (2016). https://doi.org/10.1007/s00705-015-2745-z

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  • DOI: https://doi.org/10.1007/s00705-015-2745-z

Keywords

  • Complete Genome Sequence
  • Mosaic Symptom
  • Bean Common Mosaic Virus
  • Calla Lily
  • Species Demarcation Criterion

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