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Archives of Virology

, Volume 161, Issue 2, pp 327–333 | Cite as

Protein kinase R (PKR) plays a pro-viral role in porcine reproductive and respiratory syndrome virus (PRRSV) replication by modulating viral gene transcription

  • Xiuqing Wang
  • Hanmo Zhang
  • Alex M. Abel
  • Eric Nelson
Original Article

Abstract

Protein kinase R (PKR) is involved in apoptotic cell death and antiviral activities in response to many virus infections. To reveal the role of PKR in the replication of porcine reproductive and respiratory syndrome virus (PRRSV), we first examined the kinetics of PKR phosphorylation during PRRSV infection. The results showed that PRRSV transiently activates PKR at 12 and 24 h postinfection. Surprisingly, eIF-2α, the well-known downstream target of PKR, was significantly phosphorylated compared to mock-infected cells only at 48 and 72 h postinfection. Reduced viral gene transcription, viral protein synthesis, and virus titer were detected in cells transfected with PKR silencing RNA prior to PRRSV infection compared to control silencing RNA transfected cells, indicating a role of PKR in facilitating virus replication. Overall, our data suggest that PKR is not a major contributor to the phosphorylation of eIF-2α during PRRSV infection, but it plays a pro-viral role in PRRSV replication by modulating primarily viral gene transcription.

Keywords

Chop Expression Viral Protein Synthesis Viral Gene Transcription Viral Gene Transcript Induce Stress Granule 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

This study was partly supported by grants from USDA NIFA 2012-67016-19507 and South Dakota Agricultural Experiment Station.

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Copyright information

© Springer-Verlag Wien 2015

Authors and Affiliations

  1. 1.Department of Biology and MicrobiologySouth Dakota State UniversityBrookingsUSA
  2. 2.Department of Veterinary and Biomedical SciencesSouth Dakota State UniversityBrookingsUSA

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