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Archives of Virology

, Volume 161, Issue 2, pp 335–343 | Cite as

Detection and survey of coffee ringspot virus in Brazil

  • T. O. Ramalho
  • A. R. Figueira
  • R. Wang
  • O. Jones
  • L. E. Harris
  • M. M. GoodinEmail author
Original Article

Abstract

Coffee ringspot virus (CoRSV) a member of the proposed genus “Dichorhavirus”, was surveyed on commercial and research farms spanning an area responsible for the majority of Coffea arabica production in Brazil. Virus-infected plants were found at one hundred percent of locations (n = 45) sampled. All cultivars, regardless of cherry color, were found to serve as hosts, suggesting that there is limited resistance in commercially employed germplasm. Reverse transcription PCR analysis revealed that the virus is contained within symptomatic lesions, with little systemic spread throughout leaves. Phylogenetic analysis based on the ORF1 (nucleocapsid) gene identified a strong geo-spatial relationship among isolates, which clustered into three clades. Despite low genetic diversity among isolates, variation in symptom expression was observed in the experimental host Chenopodium quinoa. Our analyses support the hypothesis that the spread of CoRSV is constrained by the clonal expansion of thelytokous populations of Brevipalpus phoenicis. The widespread occurrence of this virus suggests that it is much more prevalent than previously thought.

Keywords

Coffee Production Nucleotide Substitution Rate Coffee Rust Symptomatic Tissue Thelytokous Female 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

We thank the CNPq, CAPES, FAPEMIG, and NSF funding agencies for providing research grants to M.G. and A.F. We would also like to thank the farmers and cooperative workers who graciously provided access to their farms in support of this research. This publication recognizes CAPES Project No. A009, 1901133, and NSF-IOS-0749519).

Supplementary material

705_2015_2663_MOESM1_ESM.jpg (355 kb)
Supplementary Data 1 Combined phylogeny of CoRSV isolates using data shown in Figures 3 and 4. Sample number on the tree corresponds to collection site shown in A. Only bootstrap values greater than 60% are shown. Scale bar = number of changes per site. Collection sites of CoRSV in major coffee growing regions of Brazil; Western Minas Gerais (red), Bahia (blue) and Eastern Minas Gerais/Espírito Santo (pink). (JPEG 355 kb)

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Copyright information

© Springer-Verlag Wien 2015

Authors and Affiliations

  1. 1.Department of Plant PathologyUniversity of KentuckyLexingtonUSA
  2. 2.Departamento de FitopatologiaUniversidade Federal de LavrasLavrasBrazil

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