Skip to main content

A novel nonsegmented double-stranded RNA mycovirus identified in the phytopathogenic fungus Nigrospora oryzae shows similarity to partitivirus-like viruses

Archives of Virology volume 161, pages 229–232 (2016)Cite this article

Abstract

Nigrospora oryzae is a pathogen that can infect plants of various species. Here, we report the isolation of a novel mycovirus from N. oryzae infecting rice, as well as the complete genome sequence and genomic organization of this virus, which we have named “Nigrospora oryzae nonsegmented RNA virus 1” (NoNRV1). The genome of NoNRV1 contained two non-overlapping open reading frames (ORF1 and ORF2) potentially encoding a protein with an unknown function in ORF1 and a putative RNA-dependent RNA polymerase (RdRp) in ORF2. Homology and phylogenetic analysis revealed that NoNRV1 was most similar to the Ustilaginoidea virens nonsegmented virus 1 (UvNV-1) and distantly related to members of the virus family Partitiviridae. It is proposed that NoNRV1, together with UvNV-1 and other related viruses, might represent a novel virus taxon of mycoviruses belonging to a partitivirus-like lineage.

This is a preview of subscription content, access via your institution.

Access options

Buy single article

Instant access to the full article PDF.

43,34 €

Price includes VAT (Finland)
Tax calculation will be finalised during checkout.

Fig. 1
Fig. 2

References

  1. Ghabrial SA, Suzuki N (2009) Viruses of plant pathogenic fungi. Annu Rev Phytopathol 47:353–384

    PubMed  Article  CAS  Google Scholar 

  2. Pearson MN, Beever RE, Boine B, Arthur K (2009) Mycoviruses of filamentous fungi and their relevance to plant pathology. Mol Plant Pathol 10:115–128

    PubMed  Article  CAS  Google Scholar 

  3. Xie JT, Jiang DH (2014) New Insights into mycoviruses and exploration for the biological control of crop fungal diseases. Annu Rev Phytopathol 52:45–68

    PubMed  Article  CAS  Google Scholar 

  4. Ghabrial SA, Castón JR, Jiang D, Nibert ML, Suzuki N (2015) 50-plus years of fungal viruses. Virology 479:356–368

    PubMed  Article  CAS  Google Scholar 

  5. Nuss DL (2005) Hypovirulence: mycoviruses at the fungal–plant interface. Nat Rev Microbiol 3:632–642

    PubMed  Article  CAS  Google Scholar 

  6. Nibert ML, Ghabrial SA, Maiss E, Lesker T, Vainio EJ, Jiang D, Suzuki N (2014) Taxonomic reorganization of family Partitiviridae and other recent progress in partitivirus research. Virus Res 188:128–141

    PubMed  Article  CAS  Google Scholar 

  7. Lin Y, Zhang H, Zhao C, Liu S, Guo L (2014) The complete genome sequence of a novel mycovirus from Alternaria longipes strain HN28. Arch Virol 160:577–580

    PubMed  Article  CAS  Google Scholar 

  8. Kotta-Loizou I, Sipkova J, Coutts RH (2015) Identification and sequence determination of a novel double-stranded RNA mycovirus from the entomopathogenic fungus Beauveria bassiana. Arch Virol 160:873–875

    PubMed  Article  CAS  Google Scholar 

  9. Zheng L, Shi F, Kelly D, Hsiang T (2012) First Report of Leaf Spot of Kentucky Bluegrass (Poa pratensis) Caused by Nigrospora oryzae in Ontario. Plant Dis 96:909

    Article  Google Scholar 

  10. Sharma P, Meena PD, Chauhan JS (2013) First Report of Nigrospora oryzae (Berk. & Broome) Petch Causing Stem Blight on Brassica juncea in India. J Phytopathol 161:439–441

    Article  Google Scholar 

  11. Zhai LF, Liu J, Zhang MX, Hong N, Wang GP, Wang LP (2013) The First Report of Leaf Spots in Aloe vera Caused by Nigrospora oryzae in China. Plant Dis 97:1256

    Article  Google Scholar 

  12. Wu JB, Zhang CL, Mao PP, Qian YS, Wang HZ (2014) First report of leaf spot caused by Nigrospora oryzae on Dendrobium candidum in China. Plant Dis 98:996

    Article  Google Scholar 

  13. Zhang LX, Li SS, Tan GJ, Shen JT, He T (2012) First Report of Nigrospora oryzae causing leaf spot of cotton in China. Plant Dis 96:1379

    Article  Google Scholar 

  14. Morris TJ, Dodds JA (1979) Isolation and analysis of double stranded RNA from virus-infected plant and fungal tissue. Phytopathology 69:854–858

    Article  CAS  Google Scholar 

  15. Zhong J, Lei XH, Zhu JZ, Song G, Zhang YD, Chen Y, Gao BD (2014) Detection and sequence analysis of two novel co-infecting double-strand RNA mycoviruses in Ustilaginoidea virens. Arch Virol 159:3063–3070

    PubMed  Article  CAS  Google Scholar 

  16. Larkin MA, Blackshields G, Brown NP, Chenna R, McGettigan PA, McWilliam H, Higgins DG (2007) Clustal W and Clustal X version 2.0. Bioinformatics 23:2947–2948

    PubMed  Article  CAS  Google Scholar 

  17. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30:2725–2729

    PubMed  PubMed Central  Article  CAS  Google Scholar 

  18. Zhang T, Jiang Y, Dong W (2014) A novel monopartite dsRNA virus isolated from the phytopathogenic fungus Ustilaginoidea virens and ancestrally related to a mitochondria-associated dsRNA in the green alga Bryopsis. Virology 462:227–235

    PubMed  Article  CAS  Google Scholar 

  19. Koga R, Fukuhara T, Nitta T (1998) Molecular characterization of a single mitochondria-associated double-stranded RNA in the green alga Bryopsis. Plant Mol Biol 36:717–724

    PubMed  Article  CAS  Google Scholar 

  20. Sabanadzovic S, Valverde RA, Brown JK, Martin RR, Tzanetakis IE (2009) Southern tomato virus: the link between the families Totiviridae and Partitiviridae. Virus Res 140:130–137

    PubMed  Article  CAS  Google Scholar 

  21. Koloniuk I, Hrabáková L, Petrzik K (2015) Molecular characterization of a novel amalgavirus from the entomopathogenic fungus Beauveria bassiana. Arch Virol 160:1585–1588

    PubMed  Article  CAS  Google Scholar 

Download references

Acknowledgments

This study was supported by Hunan Provincial Innovation Foundation for Postgraduates (grant number CX2014B291).

Author information

Author notes

    Affiliations

    1. Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, Hunan Agricultural University, Nongda Road 1, Furong District, Changsha, 410128, Hunan, People’s Republic of China

      Qian Zhou, Jie Zhong, Yue Hu & Bi Da Gao

    Authors
    1. Qian Zhou
      View author publications

      You can also search for this author in PubMed Google Scholar

    2. Jie Zhong
      View author publications

      You can also search for this author in PubMed Google Scholar

    3. Yue Hu
      View author publications

      You can also search for this author in PubMed Google Scholar

    4. Bi Da Gao
      View author publications

      You can also search for this author in PubMed Google Scholar

    Corresponding author

    Correspondence to Bi Da Gao.

    Additional information

    Q. Zhou and J. Zhong contributed equally to this work.

    Electronic supplementary material

    Below is the link to the electronic supplementary material.

    Supplementary material 1 (DOCX 16 kb)

    Rights and permissions

    Reprints and Permissions

    About this article

    Verify currency and authenticity via CrossMark

    Cite this article

    Zhou, Q., Zhong, J., Hu, Y. et al. A novel nonsegmented double-stranded RNA mycovirus identified in the phytopathogenic fungus Nigrospora oryzae shows similarity to partitivirus-like viruses. Arch Virol 161, 229–232 (2016). https://doi.org/10.1007/s00705-015-2644-3

    Download citation

    • Received:

    • Accepted:

    • Published:

    • Issue Date:

    • DOI: https://doi.org/10.1007/s00705-015-2644-3

    Keywords

    • Blastp Search
    • Query Cover
    • dsRNA Virus
    • Fungal Host
    • dsRNA Segment