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Complete genome sequence of a novel endornavirus isolated from hot pepper

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Abstract

The complete genome of a putative new endornavirus infecting hot peppers (Capsicum annuum) was determined to be 14,729 nt in size, including 12 cytosines at the 3′ end. The hot pepper-infecting virus has the highest nucleotide sequence similarity (94 % query cover and 72 % identity) to bell pepper endornavirus (BPEV) isolated from the cultivar Yolo Wonder in the USA (GenBank accession no. JN019858). The putative single, large open reading frame encodes a 4,884-amino-acid-long polyprotein that contains four putative functional domains: a viral methyltransferase, a viral RNA helicase, a glycosyltransferase, and an RNA-dependent RNA polymerase. A phylogenetic tree based on whole polyprotein sequences confirmed the close evolutionary relationship of the studied endornavirus to BPEV. The hot pepper-infecting virus also has a nick at nt position 975. Taken together, these results suggest that this virus belongs to a new species in the genus Endornavirus (family Endornaviridae), for which the name hot pepper endornavirus (HPEV) is proposed.

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Acknowledgments

This research was undertaken with the support of the Cooperative Research Program for Agriculture Science & Technology Development (project no. PJ01007703), Rural Development Administration, and the Animal and Plant Quarantine Agency (QIA, project code no. Z-1542051-2013-15-01), Ministry of Agriculture, Republic of Korea.

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    Authors and Affiliations

    1. Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, 305-806, Republic of Korea

      Seungmo Lim, Fumei Zhao, Ran Hee Yoo, Davaajargal Igori & Jae Sun Moon

    2. Biosystems and Bioengineering Program, University of Science and Technology (UST), Daejeon, 305-350, Republic of Korea

      Seungmo Lim, Fumei Zhao, Ran Hee Yoo, Davaajargal Igori & Jae Sun Moon

    3. National Institute of Crop Science, Rural Development Administration, Suwon, 441-707, Republic of Korea

      Kil Hyun Kim

    4. School of Applied Biosciences, Kyungpook National University, Daegu, 702-701, Republic of Korea

      Su-Heon Lee

    5. Institute of Plant Medicine, Kyungpook National University, Daegu, 702-701, Republic of Korea

      Su-Heon Lee

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    1. Seungmo Lim
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    Correspondence to Su-Heon Lee or Jae Sun Moon.

    Additional information

    S. Lim and K. H. Kim contributed equally to this study.

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    Lim, S., Kim, K.H., Zhao, F. et al. Complete genome sequence of a novel endornavirus isolated from hot pepper. Arch Virol 160, 3153–3156 (2015). https://doi.org/10.1007/s00705-015-2616-7

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    • DOI: https://doi.org/10.1007/s00705-015-2616-7

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