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Genetic and phylogenetic analysis of a new porcine circovirus type 2 (PCV2) strain in China

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Abstract

Porcine circovirus type 2 (PCV2) is the etiological agent associated with several pig diseases that are collectively referred to as porcine circovirus-associated disease (PCVAD). Unfortunately, PCV2 has had a serious economic impact on the swine industry. In this study, we report the genome sequence of a novel PCV2 isolate (JS2015) identified in pigs in Jiang Su, China. The complete DNA sequence was 1766 nucleotides long with an A+T content of 52.7 %. It lacked a guanine (G) at nucleotide position 1045 compared to other reference PCV2 strains with a sequence length of 1766 nucleotides. Genetic characterization and phylogenetic analysis showed that the isolate JS2015 was most closely related to members of the PCV2d (AY181946) lineage. Our data provide insight into the epidemiology of porcine circovirus and may facilitate further study of the origin and evolution of PCV2.

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References

  1. Tischer I, Rasch R, Tochtermann G (1974) Characterization of papovavirus and picornavirus-like particles in permanent pig kidney cell lines. Zentralbl Bakteriol Orig A 226(2):153–167

    CAS  PubMed  Google Scholar 

  2. Hamel LA, Lin LL, Nayar GP (1998) Nucleotide sequence of porcine circovirus associated with postweaning multisystemic wasting syndrome in pigs. J Virol 72(6):5262–5267

    PubMed Central  CAS  PubMed  Google Scholar 

  3. Ellis J, Hassard L, Clark E, Hardling J, Allan G, Wilson P, Strokappe J, Martin K, McNeilly F, Meehan B, Todd D, Haines D (1998) Isolation of circovirus from lesions of pigs with postweaning multisystemic wasting syndrome. Can Vet J 39(1):44–51

    PubMed Central  CAS  PubMed  Google Scholar 

  4. Tischer I, Gelderblom H, Vettermann W, Koch MA (1982) A very small porcine virus with circular single-stranded DNA. Nature 295(5844):64–66

    Article  CAS  PubMed  Google Scholar 

  5. Franzo G, Cortey M, de Castro AM, Piovezan U, Seqales J, Driqo M, Richtzenhain LJ (2015) Genetic characterisation of porcine circovirus type 2 (PCV2) strains from feral pigs in the Brazilian Pantanal: an opportunity to reconstruct the history of PCV2 evolution. Vet Microbiol 178(1–2):158–162

    Article  CAS  PubMed  Google Scholar 

  6. Mankertz A, Mankertz J, Wolf K, Buhk HJ (1998) Identification of a protein essential for replication of porcine circovirus. J Gen Virol 79(Pt2):381–384

    Article  CAS  PubMed  Google Scholar 

  7. Nawagitgul P, Morozov I, Bolin SR, Harms PA, Sorden SD, Paul PS (2000) Open reading frame 2 of porcine circovirus type 2 encodes a major capsid protein. J Gen Virol 81(Pt9):2281–2287

    Article  CAS  PubMed  Google Scholar 

  8. Segales J, Olvera J, Grau-Roma L, Charreyre C, Nauwynck H, Larsen L, Dupont K, McCullough K, Ellis J, Krakowka S, Mankertz A, Fredholm M, Fossum C, Timmusk S, Stockhofe Zurwieden N, Beattie V, Armstrong D, Grassland B, Baekbo P, Allan G (2008) PCV2 genotype definition and nomenclature. Vet Rec 162(26):867–868

    Article  CAS  PubMed  Google Scholar 

  9. Dupont K, Nielsen EO, Baekbo P, Larsen LE (2008) Genomic analysis of PCV2 isolates from Danish archives and a current PMWS case-control study supports a shift in genotypes with time. Vet Microbiol 128(1–2):56–64

    Article  CAS  PubMed  Google Scholar 

  10. Xiao CT, Halbur PG, Opriessnig T (2012) Complete genome sequence of a novel porcine circovirus type 2b variant present in cases of vaccine failures in the United States. J Virol 86(22):12469–12473

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  11. Guo L, Fu Y, Wang Y, Lu Y, Wei Y, Tang Q, Fan P, Liu J, Zhang L, Zhang F, Huang L, Liu D, Liu C (2012) A porcine circovirus type 2 (PCV2) mutant with 234 amino acids in capsid protein showed more virulence in vivo, compared with classical PCV2a/b strain. PLoS One 7(7):e41463. doi:10.1371/journal.pone.0041463

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  12. Xiao CT, Halbur PG, Opriessnig T (2015) Global molecular genetic analysis of porcine circovirus type 2 (PCV2) sequences confirms the presence of four main PCV2 genotypes and reveals a rapid increase of PCV2d. J Gen Virol. doi:10.1099/vir.0.000100

    Google Scholar 

  13. Csagola A, Kecskemeti S, Kardos G, Kiss I, Tuboly T (2006) Genetic characterization of type 2 porcine circoviruses detected in Hungarian wild boars. Arch Virol 151(3):495–507

    Article  CAS  PubMed  Google Scholar 

  14. Seo HW, Park C, Kang I, Choi K, Jeong J, Park SJ, Chae C (2014) Genetic and antigenic characterization of a newly emerging porcine circovirus type 2b mutant first isolated in cases of vaccine failure in Korea. Arch Virol 159(11):3107–3111

    Article  CAS  PubMed  Google Scholar 

  15. An DJ, Roh IS, Song DS, Park CK, Park BK (2007) Phylogenetic characterization of porcine circovirus type 2 in PMWS and PDNS Korean pigs between 1999 and 2006. Virus Res 129(2):115–122

    Article  CAS  PubMed  Google Scholar 

  16. Cheung AK, Lager KM, Kohutyuk OI, Vincent AL, Henry SC, Baker RB, Rowland RR, Dunham AG (2007) Detection of two porcine circovirus type 2 genotypic groups in United States swine herds. Arch Virol 152(5):1035–1044

    Article  CAS  PubMed  Google Scholar 

  17. Pringle CR (1999) Virus taxonomy at the XIth International Congress of Virology, Sydney, Australia. Arch Virol 144(10):2065–2070

    Article  CAS  PubMed  Google Scholar 

  18. Wen LB, Wang FZ, He KW, Li B, Wang XM, Guo RL, Xie JP (2015) Recombination in truncated genome sequences of porcine circovirus type 2. Arch Virol 160(1):371–374

    Article  CAS  PubMed  Google Scholar 

  19. Guo J, Lu YH, Wei W, Huang LP, Liu M (2010) Porcine circovirus type 2 (PCV2): genetic variation and newly emerging genotypes in China. J Virol 7:273

    Article  Google Scholar 

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Acknowledgments

This study was funded by the National Natural Science Foundation of China (31272574,30972184) and Fund for Independent Innovation of Agricultural Sciences in Jiangsu province CX(14)2045.

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Authors and Affiliations

  1. Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, National Center for Engineering Research of Veterinary Bio-products, Nanjing, 210000, China

    Dan Zhang, Kongwang He & Libin Wen

  2. College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, China

    Hongjie Fan

Authors
  1. Dan Zhang
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  2. Kongwang He
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  3. Libin Wen
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  4. Hongjie Fan
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Correspondence to Libin Wen or Hongjie Fan.

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Zhang, D., He, K., Wen, L. et al. Genetic and phylogenetic analysis of a new porcine circovirus type 2 (PCV2) strain in China. Arch Virol 160, 3149–3151 (2015). https://doi.org/10.1007/s00705-015-2615-8

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  • DOI: https://doi.org/10.1007/s00705-015-2615-8

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