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Classical swine fever virus marker vaccine strain CP7_E2alf: genetic stability in vitro and in vivo

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Abstract

Recently, CP7_E2alf (Suvaxyn®CSF Marker), a live marker vaccine against classical swine fever virus, was licensed through the European Medicines Agency. For application of such a genetically engineered virus under field conditions, knowledge about its genetic stability is essential. Here, we report on stability studies that were conducted to assess and compare the mutation rate of CP7_E2alf in vitro and in vivo. Sequence analyses upon passaging confirmed the high stability of CP7_E2alf, and no recombination events were observed in the experimental setup. The data obtained in this study confirm the genetic stability of CP7_E2alf as an important safety component.

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Acknowledgments

The authors would like to thank Patrick Zitzow, Ulrike Kleinert and Kristin Trippler for the excellent laboratory work. The research project leading to these results has received funding through the vaccine manufacturer, Zoetis, and through the European Union’s Seventh Framework Program under grant agreement no. 289364 (RAPIDIA-Field).

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Authors and Affiliations

  1. Institute of Diagnostic Virology, Friedrich-Loeffler-Institut, Suedufer 10, 17493, Greifswald, Insel Riems, Germany

    Katja V. Goller, Carolin Dräger, Dirk Höper, Martin Beer & Sandra Blome

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  1. Katja V. Goller
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  2. Carolin Dräger
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  3. Dirk Höper
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  4. Martin Beer
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  5. Sandra Blome
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Correspondence to Sandra Blome.

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Goller, K.V., Dräger, C., Höper, D. et al. Classical swine fever virus marker vaccine strain CP7_E2alf: genetic stability in vitro and in vivo . Arch Virol 160, 3121–3125 (2015). https://doi.org/10.1007/s00705-015-2611-z

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  • DOI: https://doi.org/10.1007/s00705-015-2611-z

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