Skip to main content

Advertisement

SpringerLink
Log in

Characterization of a novel full-length bovine endogenous retrovirus, BERV-β1

  • Brief Report
  • Published:
Archives of Virology Aims and scope Submit manuscript

Abstract

Recent studies have suggested that certain classes of endogenous retroviruses (ERVs) may be present in cattle. The aim of this study was increase the scope of knowledge regarding bovine ERVs. The ovine ERV β1 pro/pol sequence was used to design a primer set for polymerase chain reaction (PCR) amplification of a similar sequence in the bovine genome. Through phylogenetic and bioinformatic analysis of the PCR product sequence together with its flanking region, a sequence 8107 bp in length was characterized. This sequence had a typical 5′-LTR-gag-pro-pol-env-LTR-3′ organization, and phylogenetic investigation defined it as a bovine ERV β1. Thus, we were able to identify a novel bovine endogenous retrovirus element.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

References

  1. Morozov VA, Morozov AV, Lagaye S (2007) Endogenous JSRV-like proviruses in domestic cattle: Analysis of sequences and transcripts. Virology 367:59–70

    Article  CAS  PubMed  Google Scholar 

  2. Baillie GJ, van de Lagemaat L, Baust NC, Mager DL (2004) Multiple groups of endogenous betaretroviruses in mice, rats, and other mammals. J Virol 78:5784–5798

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  3. Belshaw R, Pereira V, Katzourakis A, Talbot G, Paces J, Burt A, Tristem M (2004) Long-term reinfection of the human genome by endogenous retrovirus. Proc Natl Acad Sci USA 101:4894–4899

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  4. Belshaw R, Katzourakis A, Paces J, Burt A, Tristem M (2005) High copy number in human endogenous retrovirus families is associated with copying mechanisms in addition to reinfection. Mol Biol Evol 22:814–817

    Article  CAS  PubMed  Google Scholar 

  5. Griffiths DJ (2001) Endogenous retroviruses in the human genome sequence. Gen Biol 2:1017.1–1017.5

    Article  Google Scholar 

  6. Bovine Genome Sequencing and Analysis Consortium, Elsik C, Tellam R, Worley K et al (2009) The genome sequence of taurine cattle: a window to ruminant biology and evolution. Science 324:522–528

    Article  Google Scholar 

  7. Dupressoir A, Vernochet C, Bawa O, Harper F, Pierron G, Opolon P, Heidmann T (2009) Syncytin-A knockout mice demonstrate the critical role in placentation of a fusogenic, endogenous retrovirus-derived, envelope gene. Proc Natl Acad Sci USA 106:12127–12132

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  8. Patience C (1999) Endogenous retroviruses. HIV and new viruses. Academic Press, Harcourt Brace and Company, San Diego

    Google Scholar 

  9. Jern P, Coffin JM (2008) Effects of retroviruses on host genome function. Annu Rev Genet 42:709–732

    Article  CAS  PubMed  Google Scholar 

  10. Varela M, Spencer T, Palmarini M, Arnaud F (2009) Friendly viruses: the special relationship between endogenous retroviruses and their host. Ann NY Acad Sci 1178:157–172

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  11. Baba K, Nakaya Y, Shojima T, Muroi Y, Kizaki K, Hashizume K, Imakawa K, Miyazawa T (2011) Identification of novel endogenous Betaretroviruses which are transcribed in the bovine placenta. J Virol 85:1237–1245

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  12. Cornelis G, Heidemann O, Degrelle SA, Vernochet C, Lavialle C, Letzelter C et al (2013) Captured retroviral envelope syncytin gene associated with the unique placental structure of higher ruminants. Proc Natl Acad Sci USA 110:E828–E837

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  13. Gifford R, Tristem M (2003) The evolution, distribution and diversity of endogenous retroviruses. Virus Genes 26:291–315

    Article  CAS  PubMed  Google Scholar 

  14. Hayward A, Grabherr M, Jern P (2013) Broad-scale phylogenomics provides insights into retrovirus–host evolution. Proc Natl Acad Sci USA 110:20146–20151

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  15. Xiao R, Park K, Lee H, Kim J, Park C (2008) Identification and classification of endogenous retroviruses in cattle. J Virol 82:582–587

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  16. Xiao R, Park K, Oh Y, Kim J, Park C (2008) Structural characterization of the genome of BERV gamma 4, the most abundant endogenous retrovirus family in cattle. Mol Cells 26:404–408

    CAS  PubMed  Google Scholar 

  17. Garcia-Extebarria K, Jugo BM (2010) Genome-wide detection and characterization of endogenous retroviruses in Bos Taurus. J Virol 84:10852–10862

    Article  Google Scholar 

  18. Garcia-Extebarria K, Jugo BM (2013) Evolutionary history of bovine endogenous retroviruses in the Bovidae family. BMC Evol Biol 2013(13):256

    Article  Google Scholar 

  19. Klymiuk N, Müller M, Brem G, Aigner B (2003) Characterization of endogenous retroviruses in sheep. J Virol 77:11268–11273

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  20. Temin HM (1981) Structure, variation and synthesis of retrovirus long terminal repeat. Cell 27:1–3

    Article  CAS  PubMed  Google Scholar 

  21. Coffin J, Hughes S, Varmus H (1997) Retroviruses. Cold Spring Harbor Laboratory Press, New York

    Google Scholar 

  22. Xiao R, Kim J, Choi H, Park K, Lee H, Park C (2008) Characterization of the bovine endogenous retrovirus beta 3 genome. Mol Cells 25:142–147

    CAS  PubMed  Google Scholar 

  23. Baillie GJ, Wilkins RJ (2001) Endogenous type D retrovirus in a marsupial, the common brushtail possum (Trichosurus vulpecula). J Virol 75:2499–2507

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  24. Ono M, Yasunaga T, Miyata T, Ushikubo H (1986) Nucleotide sequence of human endogenous retrovirus genome related to the mouse mammary tumor virus genome. J Virol 60:589–598

    PubMed Central  CAS  PubMed  Google Scholar 

  25. Cousens C, Minguijon E, Dalziel RG, Ortin A, Garcia M, Park J, Gonzalez L, Sharp JM, de las Heras M (1999) Complete sequence of enzootic nasal tumor virus, a retrovirus associated with transmissible intranasal tumors of sheep. J Virol 73:3986–3993

    PubMed Central  CAS  PubMed  Google Scholar 

  26. Palmarini M, Hallwirth C, York D, Murgia C, de Oliveira T, Spencer T, Fan H (2000) Molecular cloning and functional analysis of three type D endogenous retroviruses of sheep reveal a different cell tropism from that of the highly related exogenous jaagsiekte sheep retrovirus. J Virol 74:8065–8076

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  27. McGeoch DJ (1990) Protein sequence comparisons show that the ‘pseudoproteases’ encoded by poxviruses and certain retroviruses belong to the deoxyuridine triphosphatase family. Nucleic Acids Res 18:4105–4110

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  28. Rosati S, Pittau M, Alberti A, Pozzi S, York DF, Sharp JM, Palmarini M (2000) An accessory open reading frame (orf-x) of jaagsiekte sheep retrovirus is conserved between different virus isolates. Virus Res 66:109–116

    Article  CAS  PubMed  Google Scholar 

  29. van der Kuyl AC (2011) Characterization of a full-length endogenous beta-retrovirus, EqERV-beta1, in the genome of the horse (Equus caballus). Viruses 3:620–628

    Article  PubMed Central  PubMed  Google Scholar 

Download references

Acknowledgments

This work was funded by the Ministry of Health, RC IZSUM 03/2010 (D. Lgs 502/92, art. 12; D.Lgs 229/99, art. 12/bis)

Author information

Authors and Affiliations

  1. Istituto Zooprofilattico Sperimentale dell’Umbria e delle Marche, via Salvemini 1, 06126, Perugia, Italy

    Claudia Torresi, Cristina Casciari, Monica Giammarioli, Francesco Feliziani & Gian Mario De Mia

Authors
  1. Claudia Torresi
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Cristina Casciari
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Monica Giammarioli
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Francesco Feliziani
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Gian Mario De Mia
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Gian Mario De Mia.

Ethics declarations

Conflict of interest

The authors declare that they have no conflict of interest.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Torresi, C., Casciari, C., Giammarioli, M. et al. Characterization of a novel full-length bovine endogenous retrovirus, BERV-β1. Arch Virol 160, 3105–3114 (2015). https://doi.org/10.1007/s00705-015-2603-z

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00705-015-2603-z

Keywords

Search

Navigation