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Deep sequencing and analysis of small RNAs in sweet orange grafted on sour orange infected with two citrus tristeza virus isolates prevalent in Sicily

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Abstract

Two representative isolates of a citrus tristeza virus population in Sicily, SG29 (aggressive) and Bau282 (mild), were sequenced via viral small RNAs (vsRNA) produced in budlings of sweet orange grafted on sour orange. Phylogenetic relationships with Mediterranean and exotic isolates revealed that SG29 clustered within the “VT-Asian” subtype, whereas Bau282 belonged to the cluster T30. The study confirms that molecular data need to be integrated with bio-indexing in order to obtain adequate information for risk assessment.

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Acknowledgments

The authors gratefully acknowledge Maria Saponari and Michela Chiumenti, CNR - Institute for Sustainable Plant Protection of Bari, Italy, for their valuable advice and for providing the custom Perl script presencePlotSize.pl, respectively.

This work was funded by MIUR through the National Program PON R&C 2007-2013, Project “IT-Citrus Genomics (PON 01_01623)”, co-funded by the EU and Project ‘Lotta al virus della tristezza degli agrumi’ funded by Assessorato delle Risorse Agricole ed Agroalimentari, Regione Siciliana.

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Correspondence to Grazia Licciardello.

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Licciardello, G., Scuderi, G., Ferraro, R. et al. Deep sequencing and analysis of small RNAs in sweet orange grafted on sour orange infected with two citrus tristeza virus isolates prevalent in Sicily. Arch Virol 160, 2583–2589 (2015). https://doi.org/10.1007/s00705-015-2516-x

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  • DOI: https://doi.org/10.1007/s00705-015-2516-x

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