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Discovery of a novel circular DNA virus in the Forbes sea star, Asterias forbesi

Abstract

A single-stranded DNA (ssDNA) virus, Asterias forbesi-associated circular virus (AfaCV), was discovered in a Forbes sea star displaying symptoms of sea star wasting disease (SSWD). The AfaCV genome organization is typical of circular Rep-encoding ssDNA (CRESS-DNA) viruses and is similar to that of members of the family Circoviridae. PCR-based surveys indicate that AfaCV is not clearly associated with SSWD, whereas the sea star-associated densovirus (SSaDV), recently implicated in SSWD in the Pacific, was prevalent in symptomatic specimens. AfaCV represents the first CRESS-DNA virus detected in echinoderms, adding to the growing diversity of these viruses recently recovered from invertebrates.

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Acknowledgments

This work was funded through grant DEB-1239976 from the National Science Foundation’s Assembling the Tree of Life Program to K.R and M.B. EF is funded by the Sanibel-Captiva Shell Club and Mary and Al Bridell Memorial Fellowship. This manuscript constitutes Sea Research Foundation publication #256.

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Correspondence to Mya Breitbart.

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Fahsbender, E., Hewson, I., Rosario, K. et al. Discovery of a novel circular DNA virus in the Forbes sea star, Asterias forbesi . Arch Virol 160, 2349–2351 (2015). https://doi.org/10.1007/s00705-015-2503-2

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  • DOI: https://doi.org/10.1007/s00705-015-2503-2

Keywords

  • Whole Genome Amplification
  • Viral Metagenomics
  • Keystone Predator
  • Major Open Reading Frame
  • Viral Contigs