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Construction and characterization of a recombinant reticuloendotheliosis virus expressing enhanced green fluorescent protein

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Abstract

Reticuloendotheliosis virus (REV) causes an immunosuppressive and oncogenic disease in chickens and other birds. In this study, based on an infectious clone of REV, named HLJR0901, a recombinant virus containing the enhanced green fluorescence protein (EGFP) gene was constructed by inserting the EGFP expression cassette downstream of the 3′ terminus of the viral env gene. An EGFP-tagged REV that stably expresses EGFP was rescued. This visible recombinant REV could contribute to the further understanding of the molecular mechanism involved in the replication and pathogenicity of REV.

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Acknowledgements

This study was funded by National Special Project on International Cooperation in Science and Technology (no. 2011DFA32210), National Agricultural Science and Technology Transformation Fund (no. 2013GB23260577), the Heilongjiang Postdoctoral Fund (no. LBH-Z12020), the State Key Laboratory Research and Business Fund of China (no. SKLVBP201409), and the Earmarked Fund for the Modern Agro-Industry Technology Research System of China (no. nycytx-42-G3-01).

Conflict of interest

The authors declare that there are no conflicts of interest regarding the publication of this paper.

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    Authors and Affiliations

    1. College of Wildlife Resources, Northeast Forestry University, Harbin, 150040, China

      Xiaoyun Deng & Yuping Hua

    2. Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, The Chinese Academy of Agricultural Sciences, Harbin, 150001, China

      Xiaoyun Deng, Feng Hu, Xiaole Qi, Li Gao, Kai Li, Honglei Gao, Yulong Gao, Yongqiang Wang, Nan Shen & Xiaomei Wang

    3. Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Disease and Zoonoses, Yangzhou, 225009, China

      Xiaomei Wang

    4. Heilongjiang Polytechnic, Harbin, 150111, China

      Xiaoyun Deng

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    1. Xiaoyun Deng
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    2. Feng Hu
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    Corresponding authors

    Correspondence to Xiaole Qi, Yuping Hua or Xiaomei Wang.

    Additional information

    X. Deng and F. Hu contributed equally to this study.

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    Deng, X., Hu, F., Qi, X. et al. Construction and characterization of a recombinant reticuloendotheliosis virus expressing enhanced green fluorescent protein. Arch Virol 160, 2231–2235 (2015). https://doi.org/10.1007/s00705-015-2502-3

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    • DOI: https://doi.org/10.1007/s00705-015-2502-3

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