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Cymbidium chlorotic mosaic virus, a new sobemovirus isolated from a spring orchid (Cymbidium goeringii) in Japan

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Abstract

Cymbidium chlorotic mosaic virus (CyCMV), isolated from a spring orchid (Cymbidium goeringii), was characterized molecularly. CyCMV isometric virions comprise a single, positive-strand RNA genome of 4,083 nucleotides and 30-kDa coat protein. The virus genome contains five overlapping open reading frames with a genomic organization similar to that of sobemoviruses. BLAST searches and phylogenetic analysis revealed that CyCMV is most closely related to papaya lethal yellowing virus, a proposed dicot-infecting sobemovirus (58.8 % nucleotide sequence identity), but has a relatively distant relationship to monocot-infecting sobemoviruses, with only modest sequence identities. This suggests that CyCMV is a new monocot-infecting member of the floating genus Sobemovirus.

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Acknowledgments

We thank K. Mitsuhata and Dr. T. Tanaka for their technical assistance, Drs. M. Tahara and N. Inouye for collecting plants, Drs. T. Kawano, S.T. Ohki, S. Yamashita and S. Toriyama for providing viruses and antisera, and Drs. T, Maeda, N. Inouye and T. Tamada for their helpful comments. This work was supported by the Ohara Foundation for Agricultural Research and Yomogi Inc.

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Author notes

  1. Sotaro Chiba

    Present address: Asian Satellite Campuses Institute, The Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, 464-8601, Japan

Authors and Affiliations

  1. Institute of Plant Science and Resources (IPSR), Okayama University, Kurashiki, 710-0046, Japan

    Hideki Kondo, Shogo Takemoto, Kazuyuki Maruyama, Sotaro Chiba, Ida Bagus Andika & Nobuhiro Suzuki

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  1. Hideki Kondo
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  2. Shogo Takemoto
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  3. Kazuyuki Maruyama
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  4. Sotaro Chiba
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Correspondence to Hideki Kondo.

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Kondo, H., Takemoto, S., Maruyama, K. et al. Cymbidium chlorotic mosaic virus, a new sobemovirus isolated from a spring orchid (Cymbidium goeringii) in Japan. Arch Virol 160, 2099–2104 (2015). https://doi.org/10.1007/s00705-015-2460-9

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  • DOI: https://doi.org/10.1007/s00705-015-2460-9

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