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Association of TNF-α gene promoter region polymorphisms in bovine leukemia virus (BLV)-infected cattle with different proviral loads


Tumor necrosis factor alpha (TNF-α) is a pleiotropic cytokine involved in the immune response against viral and other infections. Its expression levels are affected by a polymorphism in the promoter region of the gene. Bovine leukemia virus is a retrovirus that infects cattle and develops two different infection profiles in the host. One profile is characterized by a high number of proviral copies integrated into the host genome and a strong immune response against the virus, while the most relevant property of the other profile is that the number of copies integrated into the host genome is almost undetectable and the immune response is very weak. We selected a population of cattle sufficiently large for statistical analysis and classified them according to whether they had a high or low proviral load (HPL or LPL). Polymorphisms in the promoter region were identified by PCR-RFLP. The results indicated that, in the HPL group, the three possible genotypes were normally distributed and that, in the LPL group, there was a significant association between the proviral load and a low frequency of the G/G genotype at position -824.

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This work was partially supported by grants to Ceriani (Consejo Nacional de Investigación Científica y Tecnológica-PIP 577) and to Dolcini (Agencia Nacional de Promoción Científica y Tecnológica-PICT 2011 00486), and from Núcleo Consolidado CISAPA from Secretaría de Ciencia, Arte y Tecnología-Universidad Nacional Centro Provincia Buenos Aires. We thank Dr. Silvina Fernandez and Dr. Sandra Perez for helpful advice in the proofreading of this manuscript.

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Correspondence to Maria Carolina Ceriani.

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Lendez, P.A., Passucci, J.A., Poli, M.A. et al. Association of TNF-α gene promoter region polymorphisms in bovine leukemia virus (BLV)-infected cattle with different proviral loads. Arch Virol 160, 2001–2007 (2015).

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  • Bovine Leukemia Virus
  • Dairy Herd
  • Proviral Load
  • Viral Dissemination
  • Bovine Leukemia Virus Infection