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Comparison of three magnetic-bead-based RNA extraction methods for detection of cucumber green mottle mosaic virus by real-time RT-PCR

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Abstract

To determine the efficiency of RNA extraction methods based on magnetic beads, three different bead-based methods (one using silica-coated magnetic beads [SMNP], one using immunomagnetic beads conjugated to a specific antibody [IMB], and one using magnetic beads to nonspecifically adsorb virions [MNP]) were compared with the TRIzol method for the extraction of cucumber green mottle mosaic virus (CGMMV) RNA from cucumber leaves by real-time RT-PCR. The results indicated that the extraction efficiency of the SMNP method was 10 times higher than those of the IMB and MNP methods and 100 times higher than that of the TRIzol method. Therefore, the SMNP method could be considered for use in quarantine measures for the prevention and control of the disease caused by CGMMV.

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Acknowledgments

This work was supported by project funds for Automatic Detection of Plant Virus with Magnetic Nanobeads (201110035) and the Screening for Quarantine of Plant Viruses by High-Throughput Sequencing of siRNA (201310068) from the General Administration of Quality Supervision, Inspection and Quarantine of the People’s Republic of China.

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Authors and Affiliations

  1. Beijing Entry-exit Inspection and Quarantine Bureau, Tianshuiyuan Street No.6, Chaoyang District, Beijing, 100026, People’s Republic of China

    Xiaoli Zhao, Qi Zhou, Lijie Zhang, Ning Sun, Xinmiao Liang & Congliang Deng

  2. Grirem Advanced Materials Co., Ltd., National Engineering Research Center for Rare Earth Materials, General Research Institute for Nonferrous Metals, Xinjiekouwai Street No.2, Beijing, 100088, People’s Republic of China

    Wenlong Yan

Authors
  1. Xiaoli Zhao
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  2. Qi Zhou
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  3. Lijie Zhang
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  4. Wenlong Yan
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  5. Ning Sun
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  6. Xinmiao Liang
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  7. Congliang Deng
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Correspondence to Congliang Deng.

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Zhao, X., Zhou, Q., Zhang, L. et al. Comparison of three magnetic-bead-based RNA extraction methods for detection of cucumber green mottle mosaic virus by real-time RT-PCR. Arch Virol 160, 1791–1796 (2015). https://doi.org/10.1007/s00705-015-2444-9

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  • DOI: https://doi.org/10.1007/s00705-015-2444-9

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