Abstract
Grapevine leafroll-associated virus 1 (GLRaV-1) is one of the causal agents of grapevine leafroll disease (GLD). To investigate the prevalence and genetic variation of GLRaV-1 in China, 132 grapevine samples from 14 Chinese provinces and regions were tested using reverse transcription PCR (RT-PCR) and reverse transcription nested PCR (RT-nPCR). The samples included symptomatic and asymptomatic cultivars, and 36.4 % of them tested positive for GLRaV-1. ‘Beida’ samples, previously identified as virus-free rootstocks, were also found to be infected with GLRaV-1 with an incidence of 40 %. GLRaV-1 coat protein (CP) genes and heat-shock protein 70 (HSP70) genes from 43 GLRaV-1 isolates were selected and sequenced. Phylogenetic analysis of global CP and HSP70 gene sequences showed that all variants belonged to eight and seven groups, respectively. For CP gene sequence variants, group 4 was a new group that included only Chinese isolates. The results also showed that natural selection, rather than random processes, led to the evolution of variants belonging to CP gene sequence variants in group 2 and group 8. Furthermore, three new recombination events were identified in the GLRaV-1 CP gene population. This is the first report on the genetic variation of GLRaV-1 isolates in China, and this study will benefit grape clean-plant programs in China.
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Akbaş B, Kunter B, Ilhan D (2007) Occurrence and distribution of grapevine leafroll 1 associated viruses 1, 2, 3 and 7 in Turkey. J. Phytopathol 155:122–124
Al Rwahnih M, Dolja VV, Daubert S, Koonin EV, Rowhani A (2012) Genomic and biological analysis of Grapevine leafroll-associated virus 7 reveals a possible new genus within the family Closteroviridae. Virus Res 163:302–309
Alabi OJ, Rwahnih MA, Karthikeyan G, Poojari S, Fuchs M, Rowhani A, Naidu RA (2011) Grapevine leafroll-associated virus 1 occurs as genetically diverse populations. Phytopathology 101:1446–1456
Atallah SS, Gómez NI, Fuchs MF, Martensen TE (2012) Economic impact of Grapevine leafroll disease on Vitis vinifera cv. Cabernet franc in Finger Lakes vineyards of New York. Am J Enol Viticult 63:73–79
Esteves F, Teixeira Santos M, Eiras-Dias JE, Fonseca F (2013) Molecular data mining to improve antibody-based detection of Grapevine leafroll-associated virus 1 (GLRaV-1). J Virol Methods 194(1–2):258–270
Fiore N, Zamorano A, Rivera L, González F, Aballay E, Montealegre J, Pino AM (2011) Grapevine viruses in the Atacama region of Chile. J Phytopathol 159:743–750
Fu Y, Li WH (1993) Statistical tests of neutrality of mutations. Genetics 133:693–709
Gambino G, Gribaudo I (2006) Simultaneous detection of nine grapevine viruses by multiplex reverse transcription-polymerase chain reaction with coamplification of a plant RNA as internal control. Phytopathology 96:1223–1229
Hanna E, Digiaro M, Elbeaino T, Choueiri E, Jawhar J, Martelli GP (2008) Incidence of viruses and nematode vectors in Lebanese Vineyards. J Phytopathol 156:304–310
Komínek P, Glasa M, Bryxiová M (2005) Analysis of the molecular variability of Grapevine leafroll-associated Virus1 reveals the presence of two distinct virus groups and their mixed occurrence in grapevines. Virus Genes 31:247–255
Kosakovsky Pond SL, Posada D, Gravenor MB, Woelk CH, Frost SD (2006) GARD: A genetic algorithm for recombination detection. Bioinformatics 22:3096–3098
Lee J, Martin RR (2009) Influence of grapevine leafroll associated viruses (GLRaV-2 and -3) on the fruit composition of Oregon Vitis vinifera L. cv Pinot noir: phenolics. Food Chem 112:889–896
Librado P, Rozas J (2009) DnaSP v5: software for comprehensive analysis of DNA polymorphism data. Bioinformatics 25:1451–1452
Liu MH, Li MJ, Qi HH, Guo R, Liu XM, Cheng YQ (2013) Occurrence of grapevine leafroll-associated viruses in China. Plant Disease 97:1339–1345
MacKenzie DJ, Johnson RC, Warner C (1996) Incidence of four important viral pathogens in Canadian vineyards. Plant Dis 80:955–958
MacKenzie DJ, Mclean MA, Mukerji S, Grenn M (1997) Improved RNA extraction from woody plants for the detection of viral pathogens by reverse transcription-polymerase chain reaction. Plant Dis 81:222–226
Mahfoudhi N, Digiaro M, Savino V, Di Terlizzi B (1998) Viruses and virus diseases of grapevine in Tunisia. EPPO Bulletin 28:197–204
Maree HJ, Almeida RP, Bester R, Chooi KM, Cohen D, Dolja VV, Fuchs MF, Golino DA, Jooste AE, Martelli GP, Naidum RA, Rowhani A, Saldarelli P, Burger JT (2013) Grapevine leafroll-associated virus 3. Front Microbiol 4(82):1–20
Martelli GP (2000) Major graft-transmissible diseases of grapevines: nature, diagnosis, and sanitation, Proc 50th Ann ASEV Meeting. Seattle, WA. Am J Enol Vitic 51:231–236
Martelli GP, Boudon-Padieu E (2006) Directory of infectious diseases of grapevines and viruses and virus-like diseases of the grapevine: bibliographic report of 1998–2004 in Options Mediterraneennes, Serie B: N.55, CIHEAM, Bari, Italy
Martelli GP, Agranovsky AA, Al Rwahnih M, Dolja VV, Dovas CI, Fuchs M et al (2012) Taxonomic revision of the family Closteroviridae with special reference to the grapevine leafroll-associated members of the genus Ampelovirus and the putative species unassigned to the family. J Plant Pathol 94:7–19
Martin RR, Eastwell KC, Wagner A, Lamprecht S, Tzanetakis IE (2005) Survey for viruses of grapevine in Oregon and Washington. Plant Dis 89:763–766
Martin DP, Williamson C, Posada D (2005) RDP2: recombination detection and analysis from sequence alignments. Bioinformatics 21:260–262
Notomi T, Okayama H, Masubuchi H, Yonekawa T, Watanabe K, Amino N, Hase T (2000) Loop mediated isothermal amplification of DNA. Nucleic Acids Res 28(12):e63
Roossinck MJ (1997) Mechanisms of plant virus evolution. Annu Rev Phytopathol 35:1953–1965
Simon AE, Bujarski JJ (1994) RNA–RNA recombination and evolution in virus infected plants. Annu Rev Phytopathol 32:337–362
Simon-Loriere E, Holmes EC (2011) Why do RNA viruses recombine? Nature 6:617–626
Tajima F (1989) Statistical method for testing the neutral mutation hypothesis by DNA polymorphism. Genetics 123:585–595
Tamura K, Stecher G, Peterson D, Filipski A, Kumar S (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30:2725–2729
Walsh HA, Pietersen G (2013) Rapid detection of Grapevine leafroll-associated virus type 3 using a reverse transcription loop-mediated amplification method. J Virol Methods 194(1–2):308–316
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This work was supported by the earmarked fund of the China Agriculture Research System (CARS-30-bc-3).
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Fan, X., Hong, N., Dong, Y. et al. Genetic diversity and recombination analysis of grapevine leafroll-associated virus 1 from China. Arch Virol 160, 1669–1678 (2015). https://doi.org/10.1007/s00705-015-2437-8
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DOI: https://doi.org/10.1007/s00705-015-2437-8