Skip to main content

Advertisement

SpringerLink
Log in

Analysis of complete genome sequences and a V239A substitution in the helicase domain of swine hepatitis E virus strains isolated in Canada

  • Brief Report
  • Published:
Archives of Virology Aims and scope Submit manuscript

Abstract

Among Canadian swine HEV strains, only one complete genome sequence has been published so far, and there are no data on the virulence of these strains. A collection of 28 Canadian swine HEV strains was used in this study. After RNA extraction, a portion of ORF2, the 3’ end of the helicase domain, and two complete genomes were amplified and sequenced. These two new Canadian complete genomes belonged to two different subtypes and showed 87.5 and 87.7 % sequence identity to the Canadian swine HEV strain Arkell. The V239A substitution within the helicase domain, which is associated with increased virulence of genotype 3 HEV, was detected in one Canadian swine HEV strain. However, no human hepatitis E infections have been associated with this strain.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

References

  1. Aggarwal R, Krawczynski K (2000) Hepatitis E: an overview and recent advances in clinical and laboratory research. J Gastroenterol Hepatol 15:9–20

    Article  CAS  PubMed  Google Scholar 

  2. Aggarwal R (2011) Clinical presentation of hepatitis E. Virus Res 161:15–22

    Article  CAS  PubMed  Google Scholar 

  3. Ahmad I, Holla PR, Jameel S (2011) Molecular virology of hepatitis E virus. Virus Res 161:47–58

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  4. Colson P, Borentain P, Queyriaux B, Kaba M, Moal M, Gallian P, Heyries L, Raoult D, Gerolami R (2010) Pig liver sausage as a source of hepatitis E virus transmission to human. J Infect Dis 202:825–834

    Article  PubMed  Google Scholar 

  5. Halac U, Béland K, Lapierre P, Patey N, Ward P, Brassard J, Houde A, Alvare F (2012) Chronic hepatitis E infection in children with liver transplantation. Gut 61:597–603

    Article  CAS  PubMed  Google Scholar 

  6. Hakze-van der Honing RW, van Coillie E, Antonis AFG, van der Poel WHM (2011) First isolation of Hepatitis E virus genotype 4 in Europe through swine surveillance in the Netherlands and Belgium. PLoS ONE 60:e22673

    Article  Google Scholar 

  7. Huang FF, Haqshenas G, Guenette DK, Halbur PG, Schommer SK, Pierson FW, Toth TE, Meng XJ (2002) Detection by reverse transcription-PCR and genetic characterization of field isolates of swine hepatitis E virus from pigs in different geographic regions of the United States. J Clin Microbiol 40:1326–1332

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  8. Inoue J, Nishizawa T, Takahashi M, Aikawa T, Mizuo H, Suzuki K, Shimosegawa T, Okamoto H (2006) Analysis of the full-length genome of genotype 4 hepatitis E virus isolates from patients with fulminant or acute self-limited hepatitis E. J Med Virol 78:476–484

    Article  CAS  PubMed  Google Scholar 

  9. Jothikumar N, Cromeans TL, Robertson BH, Meng XJ, Hill VR (2006) A broadly reactive one-step real-time RT-PCR assay for rapid and sensitive detection of hepatitis E virus. J Virol Methods 131:65–71

    Article  CAS  PubMed  Google Scholar 

  10. Khuroo MS (2011) Discovery of hepatitis E: the epidemic non-A, non-B hepatitis 30 years down the memory lane. Virus Res 161:3–14

    Article  CAS  PubMed  Google Scholar 

  11. Leblanc D, Ward P, Gagné MJ, Poitras E, Müller P, Trottier YL, Simard C, Houde A (2007) Presence of hepatitis E virus in a naturally infected swine herd from nursery to slaughter. Int J Food Microbiol 117:160–166

    Article  CAS  PubMed  Google Scholar 

  12. Leblanc D, Houde A, Gagné MJ, Plante D, Bellon-Gagnon P, Jones TH, Muehlhauser V, Wilhelm B, Avery B, Janecko N, Brassard J (2014) Presence, viral load and characterization of Torque teno sus viruses in liver and pork chop samples at retail. Int J Food Microbiol 178:60–64

    Article  PubMed  Google Scholar 

  13. Li TC, Chijiwa K, Sera N, Ishibashi T, Etoh Y, Shinohara Y, Kurata Y, Ishida M, Sakamoto S, Takeda N, Miyamura T (2005) Hepatitis E virus transmission from wild boar meat. Emerg Infect Dis 11:1958–1960

    Article  PubMed Central  PubMed  Google Scholar 

  14. Lu L, Li C, Hagedorn CH (2006) Phylogenetic analysis of global hepatitis E virus sequences: genetic diversity, subtypes and zoonosis. Rev Med Virol 16:5–36

    Article  CAS  PubMed  Google Scholar 

  15. Meng XJ, Purcell RH, Halbur PG, Lehman JR, Webb DM, Tsareva TS, Haynes JS, Thacker BJ, Emerson SU (1997) A novel virus in swine is closely related to the human hepatitis E virus. Proc Natl Acad Sci USA 94:9860–9865

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  16. Meng XJ, Halbur PG, Shapiro MS, Govindarajan S, Bruna JD, Mushahwar IK, Purcell RH, Emerson SU (1998) Genetic and experimental evidence for cross-species infection by swine hepatitis E virus. J Virol 72:9714–9721

    CAS  PubMed Central  PubMed  Google Scholar 

  17. Mishra N, Walimbe AM, Arankalle VA (2013) Hepatitis E virus from India exhibits significant amino acid mutations in fulminant hepatic failure patients. Virus Genes 46:47–53

    Article  CAS  PubMed  Google Scholar 

  18. Mizuo H, Yazaki Y, Sugawara K, Tsuda F, Takahashi M, Nishizawa T, Okamoto H (2005) Possible risk factors for the transmission of hepatitis E virus and for the severe form of hepatitis E acquired locally in Hokkaido, Japan. J Med Virol 76:341–349

    Article  CAS  PubMed  Google Scholar 

  19. Okamoto H (2007) Genetic variability and evolution of hepatitis E virus. Virus Res 127:216–228

    Article  CAS  PubMed  Google Scholar 

  20. Panda SB, Thakral D, Rehman S (2007) Hepatitis E virus. Rev Med Virol 17:151–180

    Article  CAS  PubMed  Google Scholar 

  21. Pavio N, Meng XJ, Renou C (2010) Zoonotic hepatitis E: animal reservoirs and emerging risks. Vet Res 41:46

    Article  PubMed Central  PubMed  Google Scholar 

  22. Pei Y, Yoo D (2002) Genetic characterization and sequence heterogeneity of a Canadian isolate of swine Hepatitis E virus. J Clin Microbiol 40:4021–4029

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  23. Peralta B, Mateu E, Casas M, de Deus N, Martin M, Pina S (2009) Genetic characterization of the complete coding regions of genotype 3 hepatitis E virus isolated from Spanish swine herds. Virus Res 139:111–116

    Article  CAS  PubMed  Google Scholar 

  24. Shrestha MP, Scott RM, Joshi DM, Mammen MP, Thapa GB, Thapa N, Myint KSA, Fourneau M, Kuschner RA, Shrestha SK, David MP, Seriwatana J, Vaughn DW, Safary A, Endy TP, Innis BL (2007) Safety and efficacy of a recombinant hepatitis E vaccine. N Engl J Med 356:895–903

    Article  CAS  PubMed  Google Scholar 

  25. Smith DB, Simmonds P, Jameel S, Harrison TJ, Meng XJ, Okamoto H, Van der Poel WHM, Purdy MA (2014) Consensus proposals for classification of the family Hepeviridae. J Gen Virol 95:2223–2232

    Article  PubMed Central  PubMed  Google Scholar 

  26. Takahashi K, Kitajima N, Abe N, Mishiro S (2004) Complete or near-complete nucleotide sequences of hepatitis E virus genome recovered from a wild boar, a deer, and four patients who ate the deer. Virology 330:501–505

    Article  CAS  PubMed  Google Scholar 

  27. Takahashi K, Okamoto H, Abe N, Kawakami M, Matsuda H, Mochida S, Sakugawa H, Suginoshita Y, Watanabe S, Yamamoto K, Miyakawa Y, Mishiro S (2009) Virulent strain of hepatitis E virus genotype 3, Japan. Emerg Infect Dis 15:704–709

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  28. Tam AW, Smith MM, Guerra ME, Huang CC, Bradley DW, Fry KE, Reyes GR (1991) Hepatitis E virus (HEV): molecular cloning and sequencing of the full-length viral genome. Virology 185:120–131

    Article  CAS  PubMed  Google Scholar 

  29. Tei S, Kitajima N, Takahashi K, Mishiro S (2003) Zoonotic transmission of hepatitis E virus from deer to human beings. Lancet 362:371–373

    Article  PubMed  Google Scholar 

  30. Teo CG (2006) Hepatitis E indigenous to economically developed countries: to what extent a zoonosis? Curr Opin Infect Dis 19:460–466

    Article  PubMed  Google Scholar 

  31. Ward P, Muller P, Letellier A, Quessy S, Simard C, Trottier YL, Houde A, Brassard J (2008) Molecular characterization of hepatitis E virus detected in swine farms in the province of Quebec. Can J Vet Res 72:27–31

    CAS  PubMed Central  PubMed  Google Scholar 

  32. Yazaki Y, Mizuo H, Takahashi M, Nishizawa T, Sasaki N, Gotanda Y, Okamoto H (2003) Sporadic acute or fulminant hepatitis E in Hokkaido, Japan, may be food-borne, as suggested the presence of hepatitis E virus in pig liver as food. J Gen Virol 84:2351–2357

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

This work was financially supported by Agriculture and Agri-Food Canada Research Branch Project RBPI # 1485.

Conflict of interest

The authors declare no conflict of interest.

Author information

Authors and Affiliations

  1. Agriculture and Agri-Food Canada, Food Research and Development Centre, 3600 Casavant Blvd West, Saint-Hyacinthe, QC, J2S 8E3, Canada

    Pierre Ward, Danielle Leblanc, Alain Houde & Julie Brassard

Authors
  1. Pierre Ward
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Danielle Leblanc
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Alain Houde
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Julie Brassard
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Pierre Ward.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Ward, P., Leblanc, D., Houde, A. et al. Analysis of complete genome sequences and a V239A substitution in the helicase domain of swine hepatitis E virus strains isolated in Canada. Arch Virol 160, 1767–1773 (2015). https://doi.org/10.1007/s00705-015-2429-8

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00705-015-2429-8

Keywords

Search

Navigation