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Genome sequence of a novel endornavirus from the phytopathogenic fungus Alternaria brassicicola

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Abstract

In an effort to discover new mycoviruses from phytopathogenic fungi, a dsRNA molecule of 10,290 nt, resembling those associated with the viruses belonging to the family Endornaviridae, was isolated from Alternaria brassicicola, one of the causal agents of rapeseed black spot disease. Genome analysis revealed the presence of a single open reading frame coding for a polyprotein of 3400 aa containing conserved viral methyltransferase (MTR), viral RNA helicase 1 (Hel-1), and RNA-dependent RNA polymerase (RdRp) domains. In addition, a cysteine-rich region (CRR) with conserved CXCC motifs, shared among several endornaviruses, was also identified between the MTR and Hel-1 domains. Phylogenetic analysis based on the RdRp sequence strongly suggested that the virus infecting A. brassicicola should be considered a representative of a novel endornavirus species, and this virus was designated as Alternaria brassicicola endornavirus 1 (AbEV1).

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Acknowledgments

This study was funded by the National Key Technology R&D Program of China (2012BAD15B04-1).

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    Authors and Affiliations

    1. Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, Hunan Agricultural University, Nongda Road 1, Furong District, Changsha, 410128, China

      Hong-Hong Shang, Jie Zhong, Ru-Jia Zhang, Chuan-Yuan Chen, Bi-Da Gao & Hong-Jian Zhu

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    1. Hong-Hong Shang
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    2. Jie Zhong
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    3. Ru-Jia Zhang
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    4. Chuan-Yuan Chen
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    Corresponding authors

    Correspondence to Bi-Da Gao or Hong-Jian Zhu.

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    H.-H. Shang and J. Zhong contributed equally to this work.

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    Shang, HH., Zhong, J., Zhang, RJ. et al. Genome sequence of a novel endornavirus from the phytopathogenic fungus Alternaria brassicicola . Arch Virol 160, 1827–1830 (2015). https://doi.org/10.1007/s00705-015-2426-y

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