Skip to main content

Phylogenetic analysis of canine distemper virus in domestic dogs in Nanjing, China

Archives of Virology volume 160, pages 523–527 (2015)Cite this article

Abstract

Canine distemper virus (CDV) infects a broad range of carnivores, including wild and domestic Canidae. The hemagglutinin gene, which encodes the attachment protein that determines viral tropism, has been widely used to determine the relationship between CDV strains of different lineages circulating worldwide. We determined the full-length H gene sequences of seven CDV field strains detected in domestic dogs in Nanjing, China. A phylogenetic analysis of the H gene sequences of CDV strains from different geographic regions and vaccine strains was performed. Four of the seven CDV strains were grouped in the same cluster of the Asia-1 lineage to which the vast majority of Chinese CDV strains belong, whereas the other three were clustered within the Asia-4 lineage, which has never been detected in China. This represents the first record of detection of strains of the Asia-4 lineage in China since this lineage was reported in Thailand in 2013.

This is a preview of subscription content, access via your institution.

Access options

Buy single article

Instant access to the full article PDF.

43,34 €

Price includes VAT (Finland)
Tax calculation will be finalised during checkout.

Fig. 1
Fig. 2

References

  1. Sidhu MS, Husar W, Cook SD, Dowling PC, Udem SA (1993) Canine distemper terminal and intergenic non-protein coding nucleotide sequences: completion of the entire CDV genome sequence. Virology 193:66–72

    CAS  PubMed  Article  Google Scholar 

  2. Sun Z, Li A, Ye H, Shi Y, Hu Z, Zeng L (2010) Natural infection with canine distemper virus in hand-feeding Rhesus monkeys in China. Vet Microbiol 141:374–378

    CAS  PubMed  Article  Google Scholar 

  3. Diallo A (1990) Morbillivirus group: genome organisation and proteins. Vet Microbiol 23:155–163

    CAS  PubMed  Article  Google Scholar 

  4. Panzera Y, Calderón MG, Sarute N, Guasco S, Cardeillac A, Bonilla B, Hernández M, Francia L, Bedó G, La Torre J, Pérez R (2012) Evidence of two co-circulating genetic lineages of canine distemper virus in South America. Virus Res 163:401–404

    CAS  PubMed  Article  Google Scholar 

  5. Zhao JJ, Yan XJ, Chai XL, Martella V, Luo GL, Zhang HL, Gao H, Liu YX, Bai X, Zhang L, Chen T, Xu L, Zhao CF, Wang FX, Shao XQ, Wu W, Cheng SP (2010) Phylogenetic analysis of the haemagglutinin gene of canine distemper virus strains detected from breeding foxes, raccoon dogs and minks in China. Vet Microbiol 140:34–42

    CAS  PubMed  Article  Google Scholar 

  6. Radtanakatikanon A, Keawcharoen J, Charoenvisal NT, Poovorawan Y, Prompetchara E, Yamaguchi R, Techangamsuwan S (2013) Genotypic lineages and restriction fragment length polymorphism of canine distemper virus isolates in Thailand. Vet Microbiol 166:76–83

    CAS  PubMed  Article  Google Scholar 

  7. RaF Budaszewski, Pinto LD, Weber MN, Caldart ET, Alves CD, Martella V, Ikuta N, Lunge VR, Canal CW (2014) Genotyping of canine distemper virus strains circulating in Brazil from 2008 to 2012. Virus Res 180:76–83

    Article  Google Scholar 

  8. Han GZ, Liu XP, Li SS (2008) Cross-species recombination in the haemagglutinin gene of canine distemper virus. Virus Res 136:198–201

    CAS  PubMed  Article  Google Scholar 

  9. Martella V, Elia G, Lucente MS, Decaro N, Lorusso E, Banyai K, Blixenkrone-Møller M, Lan NT, Yamaguchi R, Cirone F, Carmichael LE, Buonavoglia C (2007) Genotyping canine distemper virus (CDV) by a hemi-nested multiplex PCR provides a rapid approach for investigation of CDV outbreaks. Vet Microbiol 122:32–42

    CAS  PubMed  Article  Google Scholar 

  10. Iwatsuki K, Tokiyoshi S, Hirayama N, Nakamura K, Ohashi K, Wakasa C, Mikami T, Kai C (2000) Antigenic differences in the H proteins of canine distemper viruses. Vet Microbiol 71:281–286

    CAS  PubMed  Article  Google Scholar 

  11. Blixenkrone-Møller M, Svansson V, Have P, Orvell C, Appel M, Pedersen IR, Dietz HH, Henriksen P (1993) Studies on manifestations of canine distemper virus infection in an urban dog population. Vet Microbiol 37:163–173

    PubMed  Article  Google Scholar 

  12. Calderon MG, Remorini P, Periolo O, Iglesias M, Mattion N, La Torre J (2007) Detection by RT-PCR and genetic characterization of canine distemper virus from vaccinated and non-vaccinated dogs in Argentina. Vet Microbiol 125:341–349

    CAS  PubMed  Article  Google Scholar 

  13. Harder TC, Osterhaus AD (1997) Canine distemper virus–a morbillivirus in search of new hosts? Trends Microbiol 5:120–124

    CAS  PubMed  Article  Google Scholar 

  14. Nikolin VM, Wibbelt G, Michler FU, Wolf P, East ML (2012) Susceptibility of carnivore hosts to strains of canine distemper virus from distinct genetic lineages. Vet Microbiol 156:45–53

    PubMed  Article  Google Scholar 

  15. Lan NT, Yamaguchi R, Kawabata A, Uchida K, Sugano S, Tateyama S (2007) Comparison of molecular and growth properties for two different canine distemper virus clusters, Asia 1 and 2, in Japan. J Vet Med Sci 69:739–744

    PubMed  Article  Google Scholar 

Download references

Acknowledgments

This work was supported by the Special Fund for Independent Innovation of Agricultural Science and Technology in Jiangsu Province of China [CX(12)3062].

Conflict of interest

None of the authors of this paper has a financial or personal relationship with other people or organizations that could inappropriately influence or bias the content of the paper.

Author information

Affiliations

  1. Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, China

    Zhenwei Bi, Yongshan Wang, Xiaoli Wang & Xingxia Xia

  2. Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Nanjing, 210014, China

    Zhenwei Bi, Yongshan Wang, Xiaoli Wang & Xingxia Xia

  3. National Center for Engineering Research of Veterinary Bio-products, Nanjing, 210014, China

    Zhenwei Bi, Yongshan Wang, Xiaoli Wang & Xingxia Xia

  4. Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, 225009, China

    Zhenwei Bi, Yongshan Wang, Xiaoli Wang & Xingxia Xia

Authors
  1. Zhenwei Bi
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Yongshan Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Xiaoli Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Xingxia Xia
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Zhenwei Bi.

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Bi, Z., Wang, Y., Wang, X. et al. Phylogenetic analysis of canine distemper virus in domestic dogs in Nanjing, China. Arch Virol 160, 523–527 (2015). https://doi.org/10.1007/s00705-014-2293-y

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00705-014-2293-y

Keywords

  • Canine distemper virus
  • Hemagglutinin (H) gene
  • Lineage
  • Phylogenetic analysis