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Complete genome sequence of three tomato ringspot virus isolates: evidence for reassortment and recombination

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Abstract

The genome sequence of tomato ringspot virus (ToRSV, a subgroup C nepovirus) is currently available for one raspberry isolate. In this study, we describe the complete genome sequence of three additional isolates from raspberry (Rasp1-2014), grapevine (GYV-2014) and prunus (13C280). The degree of nucleotide sequence identity shared between RNA1 and RNA2 in the 5′-terminal 900 nucleotides and 3′ untranslated region varied from 98-99 % (13C280, GYV-2014) to 80 % (Rasp1-2014). Phylogenetic studies revealed distinct origins for Rasp1-2014 RNA1 and RNA2, suggesting reassortment. Two recombination events were also identified in the 3′ UTR and 5′-terminal region of RNA1.

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Acknowledgments

This work was supported in part by a Natural Science and Engineering Research Council (NSERC) Discovery Grant awarded to HS, a Graduate Student Fellowship from the Chinese Science Council awarded to TW and by Genomics Research and Development Initiative (GRDI) funding awarded to MR. We thank Dr. Rowhani (UC-Davis) for sending us the original GYV isolate.

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Authors and Affiliations

  1. Pacific Agri-Food Research Centre, Agriculture and Agri-Food Canada, 4200 Highway 97, PO Box 5000, Summerland, BC, V0H 1Z0, Canada

    Melanie Walker, Joan Chisholm & Hélène Sanfaçon

  2. Department of Botany, University of British Columbia, 3529-6270 University Blvd, Vancouver, BC, V6T 1Z4, Canada

    Ting Wei, Basudev Ghoshal & Hélène Sanfaçon

  3. Sidney Laboratory, Canadian Food Inspection Agency (CFIA), 8801 East Saanich Rd, Sidney, BC, V8L 1H3, Canada

    Hanna Saeed & Michael Rott

Authors
  1. Melanie Walker
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  2. Joan Chisholm
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  3. Ting Wei
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  4. Basudev Ghoshal
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  5. Hanna Saeed
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  6. Michael Rott
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  7. Hélène Sanfaçon
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Corresponding author

Correspondence to Hélène Sanfaçon.

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Walker, M., Chisholm, J., Wei, T. et al. Complete genome sequence of three tomato ringspot virus isolates: evidence for reassortment and recombination. Arch Virol 160, 543–547 (2015). https://doi.org/10.1007/s00705-014-2240-y

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  • DOI: https://doi.org/10.1007/s00705-014-2240-y

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