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Genetic and antigenic characterization of a newly emerging porcine circovirus type 2b mutant first isolated in cases of vaccine failure in Korea

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This study describes the genetic and antigenic characterization of a newly emerging porcine circovirus type 2b (PCV2b) mutant first isolated in cases of vaccine failure in Korea. The full genome of the PCV2b isolates (SNUVR130689 and SNUVR140004) is 1,767 base pairs (bp) in length. The size of ORF1 is 945 bp, encoding a protein of 314 amino acids (aa), and the size of ORF2 is 705 bp, encoding a protein of 234 aa, which is 1 aa longer than that of the common PCV2 (233 aa). Korean PCV2b mutant strains had higher levels of nucleotide sequence identity to other PCV2b mutant strains (99.7–99.8 %) than to reference PCV2a (94.5–95.0 %) and PCV2b (95.5–96.1 %) strains. There was no difference in antigenic reactivity among PCV2a, PCV2b and PCV2b mutant strains to the polyclonal and monoclonal PCV2a antibodies. PCV2b mutant strains have distinct genetic characteristics but similar antigenic reactivity when compared to common PCV2a and 2b strains.

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  1. Allan GM, McNeilly F, Kennedy S, Daft B, Clarke EG, Ellis JA, Haines DM, Meehan BM, Adair BM (1998) Isolation of porcine circovirus-like viruses from pigs with a wasting disease in the USA and Europe. J Vet Diagn Invest 10:3–10

    Article  PubMed  CAS  Google Scholar 

  2. Chae C (2005) A review of porcine circovirus 2-associated syndromes and diseases. Vet J 169:326–336

    Article  PubMed  CAS  Google Scholar 

  3. Choi C, Han DU, Kim J, Cho WS, Chung HK, Jung T, Yoon BS, Chae C (2002) Prevalence of Brachyspira pilosicoli in Korean pigs, determined using a nested PCR. Vet Rec 150:217–218

    Article  PubMed  CAS  Google Scholar 

  4. Elder RO, Duhamel GE, Mathiesen MR, Denis Erickson E, Gebhart CJ, Oberst RD (1997) Multiplex polymerase chain reaction for simultaneous detection of Lawsonia intracellularis, Serpulina hyodysenteriae, and salmonellae in porcine intestinal specimens. J Vet Diagn Invest 9:281–286

    Article  PubMed  CAS  Google Scholar 

  5. Gagnon CA, Tremblay D, Tijssen P, Venne MH, Houde A, Elahi SM (2007) The emergence of porcine circovirus 2b genotype (PCV-2b) in swine in Canada. Can Vet J 48:811–819

    PubMed  CAS  PubMed Central  Google Scholar 

  6. Guo LJ, Lu HY, Wei YW, Huang LP, Liu CM (2010) Porcine circovirus type 2 (PCV2): genetic variation and newly emerging genotypes in China. Virol J 7:273–284

    Article  PubMed  PubMed Central  Google Scholar 

  7. Han K, Seo HW, Oh Y, Kang I, Park C, Kang SH, Kim SH, Lee BH, Kwon B, Chae C (2012) Evaluation of monoclonal antibody-based immunohistochemistry for the detection of European and North American Porcine reproductive and respiratory syndrome virus and a comparison with in situ hybridization and reverse transcription polymerase chain reaction. J Vet Diagn Invest 24:719–724

    Article  PubMed  Google Scholar 

  8. Kim D, Ha Y, Lee YH, Chae S, Lee K, Han K, Kim J, Lee JH, Kim SH, Hwang KK, Chae C (2009) Comparative study of in situ hybridization and immunohistochemistry for the detection of porcine circovirus 2 in formalin-fixed, paraffin-embedded tissues. J Vet Med Sci 71:1001–1004

    Article  PubMed  CAS  Google Scholar 

  9. Kim J, Choi C, Cho WS, Chae C (2000) Immunohistochemistry and polymerase chain reaction for the detection of Lawsonia intracellularis in porcine intestinal tissues with proliferative enteropathy. J Vet Med Sci 62:771–773

    Article  PubMed  CAS  Google Scholar 

  10. Kim O, Kim B, Chae C (1998) Prevalence of Lawsonia intracellularis in selected pig herds in Korea as determined by PCR. Vet Rec 143:587–589

    Article  PubMed  CAS  Google Scholar 

  11. McOrist S, Boid R, Lawson GHK, McConnell I (1987) Monoclonal antibodies to intracellular campylobacter-like organisms of the proliferative enteropathies. Vet Rec 121:421–422

    Article  PubMed  CAS  Google Scholar 

  12. Meehan BM, McNeilly F, Todd D, Kennedy S, Jewhurst VA, Ellis JA, Hassard LE, Clark EG, Haines DM, Allan GM (1998) Characterization of novel circovirus DNAs associated with wasting syndromes in pigs. J Gen Virol 79:2171–2179

    PubMed  CAS  Google Scholar 

  13. Opriessnig T, Xiao C-T, Gerber PF, Halbur PG (2013) Emergence of a novel mutant PCV2b variant associated with clinical PCVAD in two vaccinated pig farms in the U.S. concurrently infected with PPV2. Vet Microbiol 163:177–183

    Article  PubMed  CAS  Google Scholar 

  14. Segalés J, Olvera A, Grau-Roma L, Charreyre C, Nauwynck H, Larsen L, Dupont K, McCullough K, Ellis J, Krakowka S, Mankertz A, Fredholm M, Fossum C, Timmusk S, Stockhofe-Zurwieden N, Beattie V, Armstrong D, Grassland B, Baekbo P, Allan G (2008) PCV-2 genotype definition and nomenclature. Vet Rec 162:867–868

    Article  PubMed  Google Scholar 

  15. Seo HW, Han K, Oh Y, Park C, Chae C (2012) Efficacy of a reformulated inactivated chimeric PCV1-2 vaccine based on clinical, virological, pathological and immunological examination under field conditions. Vaccine 30:6671–6677

    Article  PubMed  CAS  Google Scholar 

  16. Xiao C-T, Halbur PG, Opriessnig T (2012) Complete genome sequence of a novel porcine circovirus type 2b variant present in cases of vaccine failures in the United States. J Virol 86:12469

    Article  PubMed  CAS  PubMed Central  Google Scholar 

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The authors’ research was supported by Technology Development Program for Agriculture and Forestry from Ministry of Agriculture, Food and Rural Affairs, Republic of Korea. This research was also supported by contract research funds of the Research Institute for Veterinary Science (RIVS) from the College of Veterinary Medicine and by BK 21 Plus Program for Creative Veterinary Science Research.

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The authors declare that they have no conflict of interest.

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Correspondence to Chanhee Chae.

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H. W. Seo and C. Park contributed equally to this work.

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Seo, H.W., Park, C., Kang, I. et al. Genetic and antigenic characterization of a newly emerging porcine circovirus type 2b mutant first isolated in cases of vaccine failure in Korea. Arch Virol 159, 3107–3111 (2014).

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