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Mixed infection by two West African tomato-infecting begomoviruses and ageratum leaf curl Cameroon betasatellite in tomato in Cameroon

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Abstract

Begomovirus isolates ToF3B2 and ToF3B17 and betasatellite isolate SatBToF3 were obtained from the same infected tomato plant showing begomovirus disease symptoms in Fontem, Cameroon. The full-length nucleotide sequences of ToF3B2, ToF3B17 and SatBToF3 were cloned and sequenced and were determined to be 2,797 nt, 2,794 and 1,373 nt long respectively. When compared with other begomovirus and betasatellite sequences, ToF3B2 was 93.5 % identical to Tomato leaf curl Togo virus, ToF3B17 was 95 % identical to Tomato leaf curl Cameroon virus and SatBToF3 was 92 % identical to Ageratum leaf curl Cameroon betasatellite (ALCCMB), respectively. The identification of ALCCMB in Ageratum and now in tomato strongly suggests Ageratum may be an alternative host to these viruses and that ALCCMB is non host specific and may cause severe diseases when transmitted to other crops.

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References

  1. Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ (1990) Basic local alignment search tool. J Mol Biol 215:403–410

    Article  PubMed  CAS  Google Scholar 

  2. Briddon RW, Brown JK, Stanley J, Zerbini M, Zhou X, Fauquet CM (2008) Recommendations for the classification and nomenclature of the DNAβ satellites of begomoviruses. Arch Virol 153:763–781

    Article  PubMed  CAS  Google Scholar 

  3. Briddon RW, Bull SE, Amin I, Mansoor S, Bedford ID, Rishi N, Siwatch SS, Zafar MY, Abdel-Salam AM, Markham PG (2004) Diversity of DNA 1: a satellite-like molecule associated with monopartite begomovirus-DNA β complexes. Virology 324:462–474

    Article  PubMed  CAS  Google Scholar 

  4. Brown JK, Fauquet CM, Briddon RW, Zerbini M, Moriones E, Navas-Castillo J (2012) Family Geminiviridae. In: King AMQ, Adams MJ, Carstens EB, Lefkowitz EJ (eds) Virus taxonomy: classification and nomenclature of viruses—ninth report of the international committee on taxonomy of viruses. Elsevier Academic Press, Oxford, pp 351–373

    Google Scholar 

  5. Bull SE, Briddon RW, Markham PG (2003) Universal primers for the PCR-mediated amplification of DNA 1: A satellite-like molecule associated with begomovirus-DNAβ complexes. Mol Biotech 23:83–86

    Article  CAS  Google Scholar 

  6. Idris AM, Brown JK (1998) Sinaloa tomato leaf curl geminivirus: biological and molecular evidence for a new subgroup III virus. Phytopathology 88:648–657

    Article  PubMed  CAS  Google Scholar 

  7. Kon T, Rojas MR, Abdourhamane IK, Gilbertson RL (2009) Roles and interactions of begomoviruses and satellite DNAs associated with okra leaf curl disease in Mali, West Africa. J Gen Virol 90:1001–1013

    Article  PubMed  CAS  Google Scholar 

  8. Leke WN, Kvarnheden A, Ngane EB, Titanji VPK, Brown JK (2011) Molecular characterization of a new begomovirus and divergent alphasatellite from tomato in Cameroon. Arch Virol 156:925–928

    Article  PubMed  CAS  Google Scholar 

  9. Leke WN, Brown JK, Ligthart ME, Sattar N, Njualem DK, Kvarnheden A (2012) Ageratum conyzoides: a host to a unique begomovirus disease complex in Cameroon. Virus Res 163:229–237

    Article  PubMed  CAS  Google Scholar 

  10. Martin DP, Lemey P, Lott M, Moulton V, Posada D, Lefeuvre P (2010) RDP3: a flexible and fast computer program for analyzing recombination. Bioinformatics 26:2462–2463

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  11. Ndunguru J, Tayor NJ, Yadav J, Aly H, Legg JP, Aveling T, Thompson G, Fauquet CM (2005) Application of FTA technology for sampling, recovery and molecular characterization of viral pathogens and virus-derived transgenes from plant tissues. Virol J 2:45

    Article  PubMed  PubMed Central  Google Scholar 

  12. Osei MK, Akromah R, Shih SL, Lee LM, Green SK (2008) First report and molecular characterization of DNA-A of three distinct begomoviruses associated with tomato leaf curl disease in Ghana. Plant Dis 92:1585

    Article  Google Scholar 

  13. Tamura K, Nei M, Kumar S (2004) Prospects for inferring very large phylogenies by using the neighbor-joining method. Proc Natl Acad Sci USA 101:11030–11035

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  14. Tamura K, Dudley J, Nei M, Kumar S (2007) MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol Biol Evol 24:1596–1599

    Article  PubMed  CAS  Google Scholar 

  15. Wyatt SD, Brown JK (1996) Detection of subgroup III geminivirus isolates in leaf extracts by degenerate primers and polymerase chain reaction. Phytopathology 86:1288–1293

    Article  Google Scholar 

  16. Zhou Y-C, Noussourou M, Kon T, Rojas MR, Jiang H, Chen LF, Gamby K, Foster R, Gilbertson RL (2008) Evidence of local evolution of tomato-infecting begomovirus species in West Africa: characterization of tomato leaf curl Mali virus and tomato yellow leaf crumple virus from Mali. Arch Virol 153:693–706

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgements

This research was supported by a research grant (number C/3913-2) from the International Foundation of Science (IFS) to W N. Leke.

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Authors and Affiliations

  1. International Institute of Tropical Agriculture (IITA), PMB 5320, Oyo road, Ibadan, Nigeria

    Walter N. Leke

  2. Institute of Agricultural Research for Development (IRAD), P.O. Box 2123, Yaounde, Cameroon

    Walter N. Leke

  3. Department of Plant Biology and Forest Genetics, Uppsala Biocenter SLU, P.O. Box 7080, 75007, Uppsala, Sweden

    Anders Kvarnheden

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  1. Walter N. Leke
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  2. Anders Kvarnheden
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Correspondence to Walter N. Leke.

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Leke, W.N., Kvarnheden, A. Mixed infection by two West African tomato-infecting begomoviruses and ageratum leaf curl Cameroon betasatellite in tomato in Cameroon. Arch Virol 159, 3145–3148 (2014). https://doi.org/10.1007/s00705-014-2159-3

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  • DOI: https://doi.org/10.1007/s00705-014-2159-3

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