Skip to main content

First report of isolation and molecular characterization of bubaline herpesvirus 1 (BuHV1) from Argentinean water buffaloes

Abstract

Herpesviruses have mainly co-evolved with their hosts for millions of years. However, bovine herpesvirus 1 (BoHV1) and related ruminant alphaherpesviruses have been reported to cross the species barrier. Bubaline herpesvirus 1 (BuHV1) is an alphaherpesvirus closely related to BoHV1 and BoHV5. According to the serological cross-relationships between ruminant alphaherpesviruses, several surveys have studied the occurrence of BoHV1-related virus infection in wild and domestic ruminant species. Recent studies in Argentina showed an increase in serological prevalence against BoHV1 related viruses in water buffaloes (Bubalus bubalis) population. The aim of this study was to investigate the presence of related ruminant alphaherpesvirus in the Argentinean water buffalo population. BuHV1 was successfully isolated from 5 out of 225 buffaloes analyzed. One isolate was obtained from nasal secretions, and the others were from vaginal swabs. The buffaloes belonged to four different farms located in northeastern Argentina. The isolates were characterized as alphaherpesvirus by direct immunofluorescence using FITC-anti-BoHV1 IgG. Restriction analysis performed with BamHI and BstEII on the complete genome showed differences between the isolates and those from BoHV1 and BoHV5 subtypes. Phylogenetic analysis on both UL27 and US6 showed similarity in tree topology. While three of the isolates grouped together with sequences of BoHV5, two other isolates clustered separately. Genetic analysis of eight concatenated sequences from all isolates and references strains showed high nucleotide sequence identity between BuHV1 and BoHV5. While three of the isolates clustered together with the BoHV5 reference strain, the last two isolates were closely related to an Australian BuHV1 strain. To our knowledge, this is the first report on the isolation and molecular characterization of BuHV1 in South America. Phylogenetic analysis suggested that two different BuHV1 lineages circulate in the Argentinean water buffalo population.

This is a preview of subscription content, access via your institution.

Fig. 1
Fig. 2
Fig. 3

References

  1. Thiry E, Muylkens B, Meurens F, Gogev S, Thiry J, Vanderplasschen A, Schynts F (2006) Recombination in the alphaherpesvirus bovine herpesvirus 1. Vet Microbiol 113:171–177

    PubMed  Article  CAS  Google Scholar 

  2. Thiry J, Keuser V, Muylkens B, Meurens F, Gogev S, Vanderplasschen A, Thiry E (2006) Ruminant alphaherpesviruses related to bovine herpesvirus 1. Vet Res 37(2):169–190

    PubMed  Article  CAS  Google Scholar 

  3. Muylkens B, Thiry J, Kirten P, Schynts F, Thiry E (2007) Bovine herpesvirus 1 infection and infectious bovine rhinotracheitis. Vet Res 38(2):181–209

    PubMed  Article  CAS  Google Scholar 

  4. Nandi S, Kumar M, Manohar M, Chauhan RS (2009) Bovine herpes virus infections in cattle. Anim Health Res Rev Conf Res Work Anim Dis 10(1):85–98

    Article  CAS  Google Scholar 

  5. St George TD, Philpott M (1972) Isolation of infectious bovine rhinotracheitis virus from the prepuce of water buffalo bulls in Australia. Aust Vet J 48:126

    PubMed  CAS  Google Scholar 

  6. Scicluna MT, Caprioli A, Saralli G, Manna G, Barone A, Cersini A, Cardeti G, Condoleo RU, Autorino GL (2010) Should the domestic buffalo (Bubalus bubalis) be considered in the epidemiology of Bovine Herpesvirus 1 infection? Vet Microbiol 143(1):81–88

    PubMed  Article  Google Scholar 

  7. Amoroso MG, Corrado F, De Carlo E, Lucibelli MG, Martucciello A, Guarino A, Galiero G (2013) Bubaline herpesvirus 1 associated with abortion in a Mediterranean water buffalo. Res Vet Sci 94(3):813–816

    PubMed  Article  CAS  Google Scholar 

  8. Peshev R, Christova L (2000) Study of bovine herpes virus 1 spreading among buffalo herds in Bulgaria. Acta Virol 44(3):229–230

    PubMed  CAS  Google Scholar 

  9. De Carlo E, Re GN, Letteriello R, Del Vecchio V, Giordanelli MP, Magnino S, Fabbi M, Bazzocchi C, Bandi C, Galiero G (2004) Molecular characterisation of a field strain of bubaline herpesvirus isolated from buffaloes (Bubalus bubalis) after pharmacological reactivation. Vet Rec 154(6):171–174

    PubMed  Article  Google Scholar 

  10. Scicluna MT, Saralli G, Bruni G, Sala M, Cocumelli C, Caciolo D, Condoleo RU, Autorino GL (2007) Epidemiological situation of herpesvirus infections in buffalo herds: bubaline herpesvirus1 or bovine herpesvirus1? Ital J Anim Sci 6:845–849

    Google Scholar 

  11. Crudeli GA, Patiño ME (2011) Milk Production from Buffaloes. In: FEPAF (ed) Origin of the Buffalo and Major Races. Cap: 1. Corrientes Argentina, pp 36–37

  12. Konrad JL, Moore DP, Crudeli G, Caspe SG, Cano DB, Leunda MR, Lischinsky L, Regidor-Cerrillo J, Odeon AC, Ortega-Mora LM et al (2012) Experimental inoculation of Neospora caninum in pregnant water buffalo. Vet Parasitol 187(1–2):72–78

    PubMed  Article  CAS  Google Scholar 

  13. Maidana SS, Mulkens B, Crudelli G, Benitez D, Thiry E, Romera SA (2010) Study of Herpesvirus in Argentinean water Buffalos [abstract]. XXVI World Buiatrics Congess. p 15

  14. Maidana SS, Lomonaco PM, Combessies G, Craig MI, Diodati J, Rodriguez D, Parreño V, Zabal O, Konrad JL, Crudeli G, Mauroy A, Thiry E, Romera SA (2012) Isolation and characterization of bovine parainfluenza virus type 3 from water buffaloes (Bubalus bubalis) in Argentina. BMC Vet Res 8:83

    PubMed  Article  PubMed Central  Google Scholar 

  15. Maidana SS, Ladelfa MF, Perez SE, Lomonaco PM, Del Medico Zajac MP, Odeon A, Blanco Viera J, Combessies G, Fondevila N, Palacios M et al (2011) Characterization of BoHV-5 field strains circulation and report of transient specific subtype of bovine herpesvirus 5 in Argentina. BMC Vet Res 7:8

    PubMed  Article  PubMed Central  Google Scholar 

  16. Del Médico Zajac MP, Romera SA, Ladelfa MF, Kotsias F, Thiry J, Ziant D, Meurens F, Keil G, Thiry E, Muylkens B (2009) Characterization of interspecific recombinants generated from closely related bovine herpesviruses 1 and 5 through multiple PCR sequencing assays. J Virol Met 161(1):75–83

    Article  Google Scholar 

  17. Ros C, Belak S (1999) Studies of genetic relationships between bovine, caprine, cervine, and rangiferine alphaherpesviruses and improved molecular methods for virus detection and identification. J Clin Microbiol 37(5):1247–1253

    PubMed  CAS  PubMed Central  Google Scholar 

  18. Thiry J, Widen F, Gregoire F, Linden A, Belak S, Thiry E (2007) Isolation and characterisation of a ruminant alphaherpesvirus closely related to bovine herpesvirus 1 in a free-ranging red deer. BMC Vet Res 3:26

    PubMed  Article  PubMed Central  Google Scholar 

  19. Hall TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucl Acids Symp Ser 41:95–98

    CAS  Google Scholar 

  20. Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28(10):2731

    PubMed  Article  CAS  PubMed Central  Google Scholar 

  21. Thiry J, Dams L, Muylkens B, Thiry E (2011) Isolation of cervid herpesvirus 1 from the genital tract of a farmed red deer in Northern France. Vet J 187(2):282–283

    PubMed  Article  Google Scholar 

Download references

Acknowledgments

The authors wish to thank Mónica Florin (INTA) and Matías Ottaviani (INTA) for help in drafting the manuscript and figures. This work was supported by Fonds de la Recherche Scientifique (FRS-FNRS) and CONICET. SM is a fellow of the CONICET (Argentina).

Conflict of interest

The authors declare that they have no conflict of interest.

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Silvina S. Maidana.

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Maidana, S.S., Konrad, J.L., Craig, M.I. et al. First report of isolation and molecular characterization of bubaline herpesvirus 1 (BuHV1) from Argentinean water buffaloes. Arch Virol 159, 2917–2923 (2014). https://doi.org/10.1007/s00705-014-2146-8

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00705-014-2146-8

Keywords

  • Water Buffalo
  • Vaginal Swab
  • MDBK Cell
  • Bovine Herpesvirus
  • Vulvovaginitis