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Phylogenetic and recombination analysis of the homing protein domain of grapevine fanleaf virus (GFLV) isolates associated with ‘yellow mosaic’ and ‘infectious malformation’ syndromes in grapevine

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Abstract

The RNA2 of seven grapevine fanleaf virus (GFLV) isolates from vines with yellow mosaic (YM) symptoms from different origin were sequenced. These sequences showed a high variability in the homing protein (2AHP) and, in five of them, a putative recombination with arabis mosaic virus (ArMV) was detected. To investigate recombination frequency, the partial sequences of the 2AHP of 28 additional GFLV isolates from nine different countries, showing either YM or infectious malformations (MF) symptoms, were obtained and compared with those of GFLV isolates from GenBank. The analysis confirmed the high level of sequence variability (up to 41 % at the nucleotide level) among isolates. In phylogenetic trees constructed using different approaches, the sequenced isolates always clustered in four conserved groups, three of which comprised YM strains (groups 1, 2 and 3), and one (group 4) the MF strains. Potential interspecific recombination sites between GFLV and ArMV were predicted in the 2AHP gene of several isolates, all of which were associated with YM symptoms.

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Authors and Affiliations

  1. Istituto Agronomico Mediterraneo di Bari, Via Ceglie 9, 70010, Valenzano, Bari, Italy

    Toufic Elbeaino, Hulusi Kiyi, Reza Boutarfa & Michele Digiaro

  2. Istituto di Virologia Vegetale del CNR, sezione di Bari, Via Amendola 165/A, 70126, Bari, Italy

    Angelantonio Minafra

  3. Dipartimento di Scienze del Suolo, della Pianta e degli Alimenti, Università degli Studi di Bari, Via Amendola 165/A, 70126, Bari, Italy

    Giovanni Paolo Martelli

Authors
  1. Toufic Elbeaino
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  2. Hulusi Kiyi
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  3. Reza Boutarfa
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  4. Angelantonio Minafra
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  5. Giovanni Paolo Martelli
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  6. Michele Digiaro
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Correspondence to Toufic Elbeaino.

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Elbeaino, T., Kiyi, H., Boutarfa, R. et al. Phylogenetic and recombination analysis of the homing protein domain of grapevine fanleaf virus (GFLV) isolates associated with ‘yellow mosaic’ and ‘infectious malformation’ syndromes in grapevine. Arch Virol 159, 2757–2764 (2014). https://doi.org/10.1007/s00705-014-2138-8

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  • DOI: https://doi.org/10.1007/s00705-014-2138-8

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