Skip to main content

Advertisement

SpringerLink
Log in

Experimental infection of cattle and goats with a foot-and-mouth disease virus isolate from the 2010 epidemic in Japan

  • Original Article
  • Published:
Archives of Virology Aims and scope Submit manuscript

Abstract

In this study, we carried out experimental infections in cattle and goats using a foot-and-mouth disease virus (FMDV) isolate from the 2010 epidemic in Japan to analyze clinical manifestations, virus-shedding patterns and antibody responses in the animals. We found that the FMDV O/JPN/2010 isolate is virulent in cattle and goats, produces clinical signs, is spread efficiently by direct contact within the same species, and is persistently infectious in cattle. Quantitative analysis of levels of viral RNA in the tissues of cattle and goats infected with the isolate showed that the pharyngeal region is an important major target of the FMDV O/JPN/2010. Time course data of viral loads, excretion and transmission of the FMDV O/JPN/2010 in this study are key in providing quantitative data essential for epidemiological investigation and risk analysis in relation to disease controls.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  1. Alexandersen S, Zhang Z, Donaldson AI (2002) Aspects of the persistence of foot-and-mouth disease virus in animals—the carrier problem. Microbes Infect 4:1099–1110

    Article  PubMed  Google Scholar 

  2. Alexandersen S, Quan M, Murphy C, Knight J, Zhang Z (2003) Studies of quantitative parameters of virus excretion and transmission in pigs and cattle experimentally infected with foot-and-mouth disease virus. J Comp Pathol 129:268–282

    Article  PubMed  CAS  Google Scholar 

  3. Alexandersen S, Zhang Z, Donaldson AI, Garland AJ (2003) The pathogenesis and diagnosis of foot-and-mouth disease. J Comp Pathol 129:1–36

    Article  PubMed  CAS  Google Scholar 

  4. Brehm KE, Ferris NP, Lenk M, Riebe R, Haas B (2009) Highly sensitive fetal goat tongue cell line for detection and isolation of foot-and-mouth disease virus. J Clin Microbiol 47:3156–3160

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  5. Donaldson D (2004) Clinical signs of foot-and-mouth disease. In: Sobrino F, Domingo E (eds) Foot and mouth disease: current perspectives. Horizon Bioscience, Wymondham, pp 93–102

    Google Scholar 

  6. Fukai K, Morioka K, Yoshida K (2011) An experimental infection in pigs using a foot-and-mouth disease virus isolated from the 2010 epidemic in Japan. J Vet Med Sci 73:1207–1210

    Article  PubMed  Google Scholar 

  7. Grubman MJ, Baxt B (2004) Foot-and-mouth disease. Clin Microbiol Rev 17:465–493

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  8. Knowles NJ, He J, Shang Y, Wadsworth J, Valdazo-González B, Onosato H, Fukai K, Morioka K, Yoshida K, Cho IS, Kim SM, Park JH, Lee KN, Luk G, Borisov V, Scherbakov A, Timina A, Bold D, Nguyen T, Paton DJ, Hammond JM, Liu X, King DP (2012) Southeast Asian foot-and-mouth disease viruses in Eastern Asia. Emerg Infect Dis 18:499–501

    Article  PubMed  PubMed Central  Google Scholar 

  9. Moniwa M, Clavijo A, Li M, Collignon B, Kitching PR (2007) Performance of a foot-and-mouth disease virus reverse transcription-polymerase chain reaction with amplification controls between three real-time instruments. J Vet Diagn Invest 19:9–20

    Article  PubMed  Google Scholar 

  10. Muroga N, Hayama Y, Yamamoto T, Kurogi A, Tsuda T, Tsutsui T (2012) The 2010 foot-and-mouth disease epidemic in Japan. J Vet Med Sci 74:399–404

    Article  PubMed  Google Scholar 

  11. Reed LJ, Muench H (1938) A simple method of estimating fifty per cent endpoints. Am J Hyg 27:493–497

    Google Scholar 

  12. Sakamoto K, Kanno T, Yamakawa M, Yoshida K, Yamazoe R, Murakami Y (2002) Isolation of foot-and-mouth disease virus from Japanese black cattle in Miyazaki Prefecture, Japan, 2000. J Vet Med Sci 64:91–94

    Article  PubMed  CAS  Google Scholar 

  13. Sugiura K, Ogura H, Ito K, Ishikawa K, Hoshino K, Sakamoto K (2001) Eradication of foot and mouth disease in Japan. Rev Sci Tech 20:701–713

    PubMed  CAS  Google Scholar 

  14. World Organization for Animal Health (2013) Chapter 2.1.5. Foot and mouth disease. Manual of diagnostic tests and vaccines for terrestrial animals 2013. http://www.oie.int/fileadmin/Home/eng/Health_standards/tahm/2.01.05_FMD.pdf. Accessed 6 Mar 2014

  15. Yamakawa M, Kanno T, Kaku Y, Morioka K, Yoshida Y, Sakamoto K (2002) Pathogenicity of a Japanese isolate of foot-and-mouth disease virus, strain O/JPN/2000, to susceptible animals. Top Anim Health Res 2:49–50 (in Japanese)

    Google Scholar 

  16. Zhang Z, Alexandersen S (2004) Quantitative analysis of foot-and-mouth disease virus RNA loads in bovine tissues: implications for the site of viral persistence. J Gen Virol 85:2567–2575

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

We are grateful to Dr. Gerelmaa Ulziibat (State Central Veterinary Laboratory, Mongolia) and Dr. Akiko Tomiyasu (Animal Quarantine Service, Japan) for technical assistance. The authors would also like to thank Mr. Hiroki Kimura, Mr. Masayuki Kanda, Mr. Shinya Sato, Mr. Kenichi Ishii, Mr. Tatsuo Nakamura and Mr. Shigeo Mizumura for care of the animals. This study was supported by Grants-in-Aid for Scientific Research from the Foot-and-Mouth Disease Control Project of the Ministry of Agriculture, Forestry and Fisheries of Japan.

Author information

Authors and Affiliations

  1. Exotic Disease Research Station, National Institute of Animal Health, National Agriculture and Food Research Organization, 6-20-1, Josui-honcho, Kodaira, Tokyo, 187-0022, Japan

    Hiroyuki Onozato, Katsuhiko Fukai, Rie Kitano, Reiko Yamazoe, Kazuki Morioka, Manabu Yamada, Seiichi Ohashi, Kazuo Yoshida & Toru Kanno

Authors
  1. Hiroyuki Onozato
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Katsuhiko Fukai
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Rie Kitano
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Reiko Yamazoe
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Kazuki Morioka
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Manabu Yamada
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Seiichi Ohashi
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Kazuo Yoshida
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Toru Kanno
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Toru Kanno.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Onozato, H., Fukai, K., Kitano, R. et al. Experimental infection of cattle and goats with a foot-and-mouth disease virus isolate from the 2010 epidemic in Japan. Arch Virol 159, 2901–2908 (2014). https://doi.org/10.1007/s00705-014-2135-y

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00705-014-2135-y

Keywords

Search

Navigation