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Thermal inactivation of Alkhumra hemorrhagic fever virus

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Abstract

The physico-chemical and biological characteristics of Alkhumra hemorrhagic fever virus (AHFV) are not yet known. The present study describes the thermal stability of this virus at different temperatures for different periods. The kinetics of thermal inactivation were studied, linear regressions were plotted, the Arrhenius equation was applied, and the activation energy was calculated accordingly. Titers of the residual virus were determined in median tissue culture infective dose (TCID50), and the rate of destruction of infectivity at various temperatures was determined. Infectivity of AHFV was completely lost upon heating for 3 minutes at 60 °C and for 30 min at 56 °C. However, the virus could maintain 33.2 % of its titer after heating for 60 min at 45 °C and 32 % of its titer after heating for 60 min at 50 °C. In conclusion, AHFV is thermo-labile, and its inactivation follows first-order kinetics.

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Abbreviations

AHFV:

Alkhumra hemorrhagic fever virus

EMEM:

Eagle’s minimum essential medium

FCS:

Fetal calf serum

TCID50 :

Median tissue culture infective dose

CPE:

Cytopathic effect

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Acknowledgments

We thank the technologist Ahmed M. Hassan for his excellent technical assistance. This work was supported by the Scientific Chair of Sheikh Mohammad Hussein Alamoudi for Viral Hemorrhagic Fever, King Abdulaziz University, Jeddah, Saudi Arabia. The sponsor, Sheikh Mohammad Hussein Alamoudi, had no involvement in the study design, in the collection, analysis or interpretation of data, in the writing of the manuscript, or in the decision to submit the manuscript for publication.

Conflict of interest

None declared.

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Authors and Affiliations

  1. Department of Medicine, Faculty of Medicine, King Abdulaziz University, PO Box 80215, Jeddah, 21589, Saudi Arabia

    Tariq A. Madani

  2. Scientific Chair of Sheikh Mohammad Hussein Alamoudi for Viral Hemorrhagic Fever, King Abdulaziz University, Jeddah, Saudi Arabia

    El-Tayb M. E. Abuelzein

  3. Special Infectious Agents Unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Saudi Arabia

    El-Tayb M. E. Abuelzein & Esam I. Azhar

  4. Department of Medical Laboratory Technology, Faculty of Applied Medical Science, King Abdulaziz University, Jeddah, Saudi Arabia

    Esam I. Azhar

  5. Department of Family and Community Medicine, Faculty of Medicine, King Abdulaziz University, Jeddah, Saudi Arabia

    Hussein M. S. Al-Bar

Authors
  1. Tariq A. Madani
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  2. El-Tayb M. E. Abuelzein
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  3. Esam I. Azhar
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  4. Hussein M. S. Al-Bar
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Correspondence to Tariq A. Madani.

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Madani, T.A., Abuelzein, ET.M.E., Azhar, E.I. et al. Thermal inactivation of Alkhumra hemorrhagic fever virus. Arch Virol 159, 2687–2691 (2014). https://doi.org/10.1007/s00705-014-2134-z

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  • DOI: https://doi.org/10.1007/s00705-014-2134-z

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