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Characterization of epizootic hemorrhagic disease virus from a bovine with clinical disease with high nucleotide sequence identity to white-tailed deer isolates

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Abstract

Epizootic hemorrhagic disease virus (EHDV) was isolated from a pregnant cow in Indiana, USA, exhibiting excessive salivation, pyrexia and abortion. VP2, VP5, and VP7 sequences of the isolated bovine EHDV showed 97.7, 97.4, and 97.9 % identity to a serotype 2 reference virus. Bovine EHDV was closely related (>99.9 %) to white tailed deer (WTD) EHDV collected from Iowa in 2013 and showed less than 2.1 % divergence from EHDV collected from WTD across the USA in 2013. The high degree of sequence identity between bovine and WTD EHDV isolates demonstrates that similar viruses concurrently circulate in both species and suggests possible further incursions into bovines.

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References

  1. Albayrak H, Ozan E, Gur S (2010) A serologic investigation of epizootic hemorrhagic disease virus (EHDV) in cattle and Gazella subgutturosa subgutturosa in Turkey. Trop Anim Health Prod 42:1589–1591

    Article  PubMed  Google Scholar 

  2. Allison AB, Goekjian VH, Potgieter AC, Wilson WC, Johnson DJ, Mertens PP, Stallknecht DE (2010) Detection of a novel reassortant epizootic hemorrhagic disease virus (EHDV) in the USA containing RNA segments derived from both exotic (EHDV-6) and endemic (EHDV-2) serotypes. J Gen Virol 91:430–439

    Article  PubMed  CAS  Google Scholar 

  3. Anbalagan S, Cooper E, Klumper P, Simonson RR, Hause BM (2014) Whole genome analysis of epizootic hemorrhagic disease virus identified limited genome constellations and preferential reassortment. J Gen Virol 95:434–441

    Article  PubMed  CAS  Google Scholar 

  4. Anthony SJ, Maan S, Maan N, Kgosana L, Bachanek-Bankowska K, Batten C, Darpel KE, Sutton G, Attoui H, Mertens PP (2009) Genetic and phylogenetic analysis of the outer-coat proteins VP2 and VP5 of epizootic haemorrhagic disease virus (EHDV): comparison of genetic and serological data to characterise the EHDV serogroup. Virus Res 145:200–210

    Article  PubMed  CAS  Google Scholar 

  5. Aradaib IE, Mederos RA, Osburn BI (2005) Evaluation of epizootic haemorrhagic disease virus infection in sentinel calves from the San Joaquin Valley of California. Vet Res Commun 29:447–451

    Article  PubMed  CAS  Google Scholar 

  6. Batten CA, Edwards L, Bin-Tarif A, Henstock MR, Oura CA (2011) Infection kinetics of Epizootic haemorrhagic disease virus serotype 6 in Holstein-Friesian cattle. Vet Microbiol 154:23–28

    Article  PubMed  CAS  Google Scholar 

  7. Boyer TC, Ward MP, Singer RS (2010) Climate, landscape, and the risk of orbivirus exposure in cattle in Illinois and western Indiana. Am J Trop Med Hyg 83:789–794

    Article  PubMed  PubMed Central  Google Scholar 

  8. Cheney IW, Yamakawa M, Roy P, Mecham JO, Wilson WC (1996) Molecular characterization of the segment 2 gene of epizootic hemorrhagic disease virus serotype 2: gene sequence and genetic diversity. Virology 224:555–560

    Article  PubMed  CAS  Google Scholar 

  9. Clavijo A, Sun F, Lester T, Jasperson DC, Wilson WC (2010) An improved real-time polymerase chain reaction for the simultaneous detection of all serotypes of Epizootic hemorrhagic disease virus. J Vet Diagn Invest 22:588–593

    Article  PubMed  Google Scholar 

  10. Dubay SA, deVos JC Jr, Noon TH, Boe S (2004) Epizootiology of hemorrhagic disease in mule deer in central Arizona. J Wildl Dis 40:119–124

    Article  PubMed  Google Scholar 

  11. Dubay SA, Noon TH, deVos JC Jr, Ockenfels RA (2006) Serologic survey for pathogens potentially affecting pronghorn (Antilocapra americana) fawn recruitment in Arizona, USA. J Wildl Dis 42:844–848

    Article  PubMed  Google Scholar 

  12. Dunbar MR, Velarde R, Gregg MA, Bray M (1999) Health evaluation of a pronghorn antelope population in Oregon. J Wildl Dis 35:496–510

    Article  PubMed  CAS  Google Scholar 

  13. Eschbaumer M, Wernike K, Batten CA, Savini G, Edwards L, Di Gennaro A, Teodori L, Oura CA, Beer M, Hoffmann B (2012) Epizootic hemorrhagic disease virus serotype 7 in European cattle and sheep: diagnostic considerations and effect of previous BTV exposure. Vet Microbiol 159:298–306

    Article  PubMed  Google Scholar 

  14. Gaydos JK, Crum JM, Davidson WR, Cross SS, Owen SF, Stallknecht DE (2004) Epizootiology of an epizootic hemorrhagic disease outbreak in West Virginia. J Wildl Dis 40:383–393

    Article  PubMed  Google Scholar 

  15. Gibbs EP, Lawman MJ (1977) Infection of British deer and farm animals with epizootic haemorrhagic disease of deer virus. J Comp Pathol 87:335–343

    Article  PubMed  CAS  Google Scholar 

  16. House C, Shipman LD, Weybright G (1998) Serological diagnosis of epizootic hemorrhagic disease in cattle in the USA with lesions suggestive of vesicular disease. Ann N Y Acad Sci 849:497–500

    Article  PubMed  CAS  Google Scholar 

  17. Inaba U (1975) Ibaraki disease and its relationship to bluetongue. Aust Vet J 51:178–185

    Article  PubMed  CAS  Google Scholar 

  18. Murphy MD, Howerth EW, MacLachlan NJ, Stallknecht DE (2005) Genetic variation among epizootic hemorrhagic disease viruses in the southeastern United States: 1978–2001. Infect Genet Evol 5:157–165

    Article  PubMed  CAS  Google Scholar 

  19. Murphy MD, Hanson BA, Howerth EW, Stallknecht DE (2006) Molecular characterization of epizootic hemorrhagic disease virus serotype 1 associated with a 1999 epizootic in white-tailed deer in the eastern United States. J Wildl Dis 42:616–624

    Article  PubMed  CAS  Google Scholar 

  20. Nol P, Kato C, Reeves WK, Rhyan J, Spraker T, Gidlewski T, VerCauteren K, Salman M (2010) Epizootic hemorrhagic disease outbreak in a captive facility housing white-tailed deer (Odocoileus virginianus), bison (Bison bison), elk (Cervus elaphus), cattle (Bos taurus), and goats (Capra hircus) in Colorado, USA. J Zoo Wildl Med 41:510–515

    Article  PubMed  Google Scholar 

  21. Nusinovici S, Seegers H, Joly A, Beaudeau F, Fourichon C (2012) Increase in the occurrence of abortions associated with exposure to the Bluetongue virus serotype 8 in naive dairy herds. Theriogenology 78:1140–1151

    Article  PubMed  CAS  Google Scholar 

  22. Ohashi S, Yoshida K, Watanabe Y, Tsuda T (1999) Identification and PCR-restriction fragment length polymorphism analysis of a variant of the Ibaraki virus from naturally infected cattle and aborted fetuses in Japan. J Clin Microbiol 37:3800–3803

    PubMed  CAS  PubMed Central  Google Scholar 

  23. Ohashi S, Yoshida K, Yanase T, Tsuda T (2002) Analysis of intratypic variation evident in an Ibaraki virus strain and its epizootic hemorrhagic disease virus serogroup. J Clin Microbiol 40:3684–3688

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  24. Omori T, Inaba Y, Morimoto T, Tanaka Y, Ishitani R (1969) Ibaraki virus, an agent of epizootic disease of cattle resembling bluetongue. I. Epidemiologic, clinical and pathologic observations and experimental transmission to calves. Jpn J Microbiol 13:139–157

    Article  PubMed  CAS  Google Scholar 

  25. Potgieter AC, Page NA, Liebenberg J, Wright IM, Landt O, van Dijk AA (2009) Improved strategies for sequence-independent amplification and sequencing of viral double-stranded RNA genomes. J Gen Virol 90:1423–1432

    Article  PubMed  CAS  Google Scholar 

  26. Santman-Berends IM, Hage JJ, van Rijn PA, Stegeman JA, van Schaik G (2010) Bluetongue virus serotype 8 (BTV-8) infection reduces fertility of Dutch dairy cattle and is vertically transmitted to offspring. Theriogenology 74:1377–1384

    Article  PubMed  CAS  Google Scholar 

  27. Savini G, Afonso A, Mellor P, Aradaib I, Yadin H, Sanaa M, Wilson W, Monaco F, Domingo M (2011) Epizootic heamorragic disease. Res Vet Sci 91:1–17

    Article  PubMed  CAS  Google Scholar 

  28. Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  29. Temizel EM, Yesilbag K, Batten C, Senturk S, Maan NS, Clement-Mertens PP, Batmaz H (2009) Epizootic hemorrhagic disease in cattle, Western Turkey. Emerg Infect Dis 15:317–319

    Article  PubMed  PubMed Central  Google Scholar 

  30. Uchinuno Y, Ito T, Goto Y, Miura Y, Ishibashi K, Itou T, Sakai T (2003) Differences in Ibaraki virus RNA segment 3 sequences from three epidemics. J Vet Med Sci 65:1257–1263

    Article  PubMed  CAS  Google Scholar 

  31. Van Campen H, Davis C, Flinchum JD, Bishop JV, Schiebel A, Duncan C, Spraker T (2013) Epizootic hemorrhagic disease in yaks (Bos grunniens). J Vet Diagn Invest 25:443–446

    Article  PubMed  Google Scholar 

  32. Weir RP, Harmsen MB, Hunt NT, Blacksell SD, Lunt RA, Pritchard LI, Newberry KM, Hyatt AD, Gould AR, Melville LF (1997) EHDV-1, a new Australian serotype of epizootic haemorrhagic disease virus isolated from sentinel cattle in the Northern Territory. Vet Microbiol 58:135–143

    Article  PubMed  CAS  Google Scholar 

  33. Yadin H, Brenner J, Bumbrov V, Oved Z, Stram Y, Klement E, Perl S, Anthony S, Maan S, Batten C, Mertens PP (2008) Epizootic haemorrhagic disease virus type 7 infection in cattle in Israel. Vet Rec 162:53–56

    Article  PubMed  CAS  Google Scholar 

  34. Zanella G, Durand B, Sellal E, Breard E, Sailleau C, Zientara S, Batten CA, Mathevet P, Audeval C (2012) Bluetongue virus serotype 8: abortion and transplacental transmission in cattle in the Burgundy region, France, 2008–2009. Theriogenology 77:65–72

    Article  PubMed  CAS  Google Scholar 

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Author information

Authors and Affiliations

  1. Newport Laboratories, 1520 Prairie Drive, Worthington, MN, 56187, USA

    Srivishnupriya Anbalagan

  2. Veterinary Diagnostic Laboratory, Kansas State University, Manhattan, KS, 66506, USA

    Ben M. Hause

Authors
  1. Srivishnupriya Anbalagan
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  2. Ben M. Hause
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Corresponding author

Correspondence to Ben M. Hause.

Additional information

The GenBank accession numbers for the bovine EHDV genomic RNA segments are KJ125190- KJ125199.

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Anbalagan, S., Hause, B.M. Characterization of epizootic hemorrhagic disease virus from a bovine with clinical disease with high nucleotide sequence identity to white-tailed deer isolates. Arch Virol 159, 2737–2740 (2014). https://doi.org/10.1007/s00705-014-2120-5

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  • DOI: https://doi.org/10.1007/s00705-014-2120-5

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