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A broadly reactive monoclonal antibody detects multiple genotypes of hepatitis B virus X protein

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Abstract

A highly specific and broadly reactive monoclonal antibody against hepatitis B virus X (HBx) protein was developed that detected, in both immunoblotting and immunofluorescence, HBx proteins of seven of the eight currently known genotypes of HBV, which were overexpressed in cultured cells. Evaluation of HBx expression levels in cultured hepatocytes using this monoclonal antibody showed that cells transiently and stably transfected with HBV genomes expressed far less HBx protein than cells transiently transfected with an HBx overexpression plasmid routinely used for studying HBx functions. The availability of such sensitive and broadly reactive monoclonal antibodies against HBx will enable more-quantitative studies of HBx functions.

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Acknowledgments

This work was supported by NSFC (31071143, 31170148), the National Science and Technology Major Project for Infectious Diseases (2012ZX10002-006, 2012ZX10004-503 and 2013ZX10002-001), “973” program (2012CB519002), Shanghai Science and Technology Committee (11DZ2291900).

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Authors and Affiliations

  1. Key Laboratory of Medical Molecular Virology (MOE/MOH) and Institutes of Biomedical Sciences, Shanghai Medical College, Fudan University, Shanghai, 200032, People’s Republic of China

    Lili Wei, Zhongliang Shen, Xue Zhao, Yanxin Wu, Wei Liu, Junqi Zhang, Youhua Xie & Jing Liu

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  1. Lili Wei
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  2. Zhongliang Shen
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  3. Xue Zhao
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  4. Yanxin Wu
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  5. Wei Liu
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  6. Junqi Zhang
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  7. Youhua Xie
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  8. Jing Liu
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Corresponding authors

Correspondence to Youhua Xie or Jing Liu.

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Wei, L., Shen, Z., Zhao, X. et al. A broadly reactive monoclonal antibody detects multiple genotypes of hepatitis B virus X protein. Arch Virol 159, 2731–2735 (2014). https://doi.org/10.1007/s00705-014-2111-6

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  • DOI: https://doi.org/10.1007/s00705-014-2111-6

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