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Effects of a hemagglutinin D222G substitution on the pathogenicity of 2009 influenza A (H1N1) virus in mice

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Abstract

The surface glycoprotein hemagglutinin (HA) of influenza virus initiates the infection process by binding to sialic acid receptors on upper respiratory cells in the host. In contrast to avian influenza viruses, which bind to sialic acids connected by an α2-3 linkage to the penultimate galactose, human influenza viruses prefer sialic acids with an α2-6 linkage. Recently, there have been multiple cases of severe human infections associated with an HA D222G mutant influenza virus. In this study, we have investigated the pathogenic effects of the HA D222G substitution in a 2009 pandemic H1N1 virus in mice. Compared with the A/Korea/01/2009 (K/09) virus, the HA D222G mutant showed reduced growth in cells and reduced binding avidity to human and turkey red blood cells. In a BALB/c mouse infection model, infection with the HA D222G mutant virus resulted in less body weight loss when compared to the parental K/09 virus. Altogether, our data suggest that the HA D222G substitution in the K/09 virus might be deleterious to viral fitness.

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Acknowledgments

This study was supported by a grant from the Korea Healthcare Technology R&D Project of the Ministry of Health & Welfare (Grant No. A103001).

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Authors and Affiliations

  1. Department of Microbiology, College of Medicine, and the Institute for Viral Diseases, Korea University, Seoul, 136-705, Republic of Korea

    Jin Il Kim, Ilseob Lee, Sehee Park, Sangmoo Lee, Min-Woong Hwang, Joon-Yong Bae, Jun Heo, Jin-Won Song & Man-Seong Park

  2. Department of Microbiology, College of Medicine, Hallym University, Chuncheon, Gangwon-do, 200-702, Republic of Korea

    Jin Il Kim, Ilseob Lee, Sehee Park, Sangmoo Lee, Min-Woong Hwang, Joon-Yong Bae, Jun Heo, Donghwan Kim, Seok-Il Jang & Man-Seong Park

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  1. Jin Il Kim
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Correspondence to Man-Seong Park.

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J. I. Kim and I. Lee contributed equally to this article.

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Kim, J.I., Lee, I., Park, S. et al. Effects of a hemagglutinin D222G substitution on the pathogenicity of 2009 influenza A (H1N1) virus in mice. Arch Virol 159, 2559–2565 (2014). https://doi.org/10.1007/s00705-014-2104-5

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