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Characterization of a thermophilic bacteriophage of Geobacillus kaustophilus

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Abstract

GBK2 is a bacteriophage, isolated from a backyard compost pile, that infects the thermophile Geobacillus kaustophilus. GBK2 has a circularly permuted genome of 39,078 bp with a G+C content of 43 %. Annotation of the genome reveals 62 putative open reading frames (ORFs), 25 of which (40.3 %) show homology to known proteins and 37 of which (59.7 %) are proteins with unknown functions. Twelve of the identified ORFs had the greatest homology to genes from the phage SPP1, a phage that infects the mesophile Bacillus subtilis. The overall genomic arrangement of GBK2 is similar to that of SPP1, with the majority of GBK2 SPP1-like genes coding for proteins involved in DNA replication and metabolism.

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Acknowledgments

The authors would like to thank the Genomic Sciences Laboratory (GSL) at NCSU for assistance in sequence determination and assembly.

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Correspondence to Paul T. Hamilton.

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Marks, T.J., Hamilton, P.T. Characterization of a thermophilic bacteriophage of Geobacillus kaustophilus . Arch Virol 159, 2771–2775 (2014). https://doi.org/10.1007/s00705-014-2101-8

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  • DOI: https://doi.org/10.1007/s00705-014-2101-8

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