Skip to main content

A raspberry bushy dwarf virus isolate from Ecuadorean Rubus glaucus contains an additional RNA that is a rearrangement of RNA-2

Abstract

Sequencing of the complete genome of a raspberry bushy dwarf virus isolate from Rubus glaucus in Ecuador revealed that its RNA-1 and RNA-2 were 5449 and 2231 nucleotides (nt) long, respectively, and phylogenetically closest to isolates from Sweden and Slovenia. In dsRNA analysis of infected plants, an additional band of 3 kbp was observed. Sequencing of this band revealed that it was 3279 nt long. BLAST searches revealed that this band contained a modified version of RNA-2, which consisted of RNA-2 (2231 nt) plus an additional 1048-nt fragment that was concatenated in a reverse-complement fashion to its 5’ terminus.

This is a preview of subscription content, access via your institution.

Fig. 1
Fig. 2

References

  1. Attoui H, Billoir F, Cantaloube JF, Biagini P, de Micco P, de Lamballerie X (2000) Strategies for the sequence determination of viral dsRNA genomes. J Virol Methods 89:147–158

    CAS  PubMed  Article  Google Scholar 

  2. Froussard P (1992) A random-PCR method (rPCR) to construct whole cDNA library from low amounts of RNA. Nucleic Acids Res 20:2900

    CAS  PubMed Central  PubMed  Article  Google Scholar 

  3. Jones AT, McGavin WJ, Mayo MA, Angel-Diaz JE, Karenlampi SO, Kokko H (2000) Comparison of some properties of two laboratory variants of raspberry bushy dwarf virus (RBDV) with those of three previously characterized RBDV isolates. Eur J Plant Pathol 106:623–632

    Article  Google Scholar 

  4. Μartin RR, MacFarlane S, Sabanadzovic S, Quito-Avila DF, Poudel B, Tzanetakis IE (2013) Viruses and virus diseases of Rubus. Plant Dis 97:168–182

    Article  Google Scholar 

  5. Mavrič I, Viršček-Marn M, Koron D (2003) First report of Raspberry bushy dwarf virus on red raspberry and grapevine in Slovenia. Plant Dis 87:1148

    Article  Google Scholar 

  6. Mavric-Plesko I, Virscek-Marn M, Sirca S, Urek G (2009) Biological, serological and molecular characterisation of Raspberry bushy dwarf virus from grapevine and its detection in the nematode Longidorus juvenilis. Eur J Plant Pathol 123:261–268

    Article  Google Scholar 

  7. Mavrič-Plesko I, Viršček-Marn M (2012) First report of Raspberry bushy dwarf virus infecting grapevine in Hungary. Plant Dis 96:1582

    Article  Google Scholar 

  8. Mayo MA, Jolly CA, Murant AF, Raschke JH (1991) Nucleotide sequence of raspberry bushy dwarf virus RNA-3. J Gen Virol 72:469–472

    CAS  PubMed  Article  Google Scholar 

  9. Morris TJ, Dodds JA (1979) Isolation and analysis of double-stranded RNA from virus-infected plant and fungal tissue. Phytopathology 69:854–858

    CAS  Article  Google Scholar 

  10. Murant AF, Mayo MA, Raschke JH (1986) Some biochemical properties of raspberry bushy dwarf virus. Acta Hortic 186:23–30

    Google Scholar 

  11. Natsuaki T, Mayo MA, Jolly CA, Murant AF (1991) Nucleotide sequence of raspberry bushy dwarf virus RNA-2: a bicistronic component of a bipartite genome. J Gen Virol 72:2183–2189

    CAS  PubMed  Article  Google Scholar 

  12. Quito-Avila DF, Peralta EL, Ibarra MA, Alvarez R, Ratti MF, Espinoza L, Martin RR (2013) First report of Raspberry bushy dwarf virus in blackberry in Ecuador. Plant Dis 97:1003

    Google Scholar 

  13. Valasevich N, Kukharchyk N, Kvarnheden A (2011) Molecular characterization of Raspberry bushy dwarf virus isolates from Sweden and Belarus. Arch Virol 156:369–374

    CAS  PubMed  Article  Google Scholar 

  14. Ziegler A, Natsuaki T, Mayo MA, Jolly CA, Murant AF (1992) The nucleotide sequence of RNA-1 of raspberry bushy dwarf virus. J Gen Virol 73:3213–3218

    CAS  PubMed  Article  Google Scholar 

Download references

Acknowledgments

This research was supported in part by the Ecuadorean Science and Technology Secretariat (SENESCYT) through the PROMETEO program. The authors also thank blackberry growers in Paute-Azuay and Tisaleo-Tungurahua for granting us access to their fields.

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to D. F. Quito-Avila.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Quito-Avila, D.F., Ibarra, M.A., Alvarez, R. et al. A raspberry bushy dwarf virus isolate from Ecuadorean Rubus glaucus contains an additional RNA that is a rearrangement of RNA-2. Arch Virol 159, 2519–2521 (2014). https://doi.org/10.1007/s00705-014-2069-4

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00705-014-2069-4

Keywords

  • Coat Protein Gene
  • Movement Protein
  • Black Raspberry
  • Movement Protein Gene
  • Canadian Isolate